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Hck Antibody (1D9-1A9)

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Western Blot: Hck Antibody (1D9-1A9) [H00003055-M01] - Analysis of HCK expression in transfected 293T cell line by HCK monoclonal antibody (M01), clone 1D9-1A9.Lane 1: HCK transfected lysate (Predicted MW: 57.3 KDa).Lane 2: Non-transfected lysate.
Western Blot: Hck Antibody (1D9-1A9) [H00003055-M01] - Analysis of HCK expression in transfected 293T cell line by HCK monoclonal antibody (M01), clone 1D9-1A9.Lane 1: HCK transfected lysate (Predicted MW: 57.3 ...read more
Immunoprecipitation: Hck Antibody (1D9-1A9) [H00003055-M01] - Analysis of HCK transfected lysate using anti-HCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HCK MaxPab rabbit polyclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, IP
Clone
1D9-1A9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

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Hck Antibody (1D9-1A9) Summary

Description
Quality control test: Antibody Reactive Against Recombinant Protein.
Immunogen
HCK (AAH14435, 1 a.a. ~ 505 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MGCMKSKFLQVGGNTSSKTETSASPHCPVYVPDPTSTIKPGPNSHNSNTPGIREAGSEDIIVVALYDYEAIHHEDLSFQKGDQMVVLEESGEWWKARSLATRKEGYIPSNYVARVDSLETEEWFFKGISRKDAERQLLAPGNMLGSFMIRDSETTKGSYSLSVRDYDPRQGDTVKHYKIRTLDNGGFYISPRSTFSTLQELVDHYKKGNDGLCQKLSVPCMSSKPQKPWEKDAWEIPRESLKLEKKLGAGQFGEVWMATYNKHTKVAVKTMKPGSMSVEAFLAEANVMKTLQHDKLVKLHAVVTKEPIYIITEFMAKGSLLDFLKSDEGSKQPLPKLIDFSAQIAEGMAFIEQRNYIHRDLRAANILVSASLVCKIADFGLARVIEDNEYTAREGAKFPIKWTAPEAINFGSFTIKSDVWSFGILLMEIVTYGRIPYPGVSNPEVIRALERGYRMPRPENCPEELYNIMMRCWKNRPEERPTFEYIQSVLDDFYTATESQYQQQP
Localization
Isoform p60-HCK and isoform p59-HCK are associated with membranes. Isoform p60-HCK is also cytoplasmic.
Specificity
HCK - hemopoietic cell kinase
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
HCK
Purity
IgG purified
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Applications/Dilutions

Dilutions
  • ELISA
  • Immunoprecipitation
  • Western Blot 1:500
Application Notes
Antibody reactivity against Recombinant Protein with GST tag on ELISA and WB. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
In 1x PBS, pH 7.4
Preservative
No Preservative
Purity
IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for Hck Antibody (1D9-1A9)

  • Bmk
  • EC 2.7.10
  • EC 2.7.10.2
  • Hck
  • Hctk
  • hemopoietic cell kinaseJTK9
  • JTK9
  • p59-HCK/p60-HCK
  • tyrosine-protein kinase HCK

Background

The protein encoded by this gene is a protein-tyrosine kinase that is predominantly expressed in hemopoietic cell types. The encoded protein may help couple the Fc receptor to the activation of the respiratory burst. In addition, it may play a role in neutrophil migration and in the degranulation of neutrophils. Alternate translation initiation site usage, including a non-AUG (CUG) codon, results in the production of two different isoforms, that have different subcellular localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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H00003055-M01
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Bioinformatics

Gene Symbol HCK
Entrez
OMIM
Uniprot