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HB-EGF Antibody [Biotin]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF-259-NA
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF-259-NA
Carrier Free
AF-259-NA
Carrier Free
AF-259-SP
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HB-EGF Antibody [Biotin] Summary

Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human HB-EGF (R&D Systems, Catalog # 259-HE)
Asp63-Leu148
Accession # Q99075
Specificity
Detects human HB-EGF in ELISAs and Western blots. In direct ELISAs, less than 1% cross reactivity with recombinant mouse HB-EGF is observed. In sandwich immunoassays, less than 0.1% cross-reactivity with recombinant human (rh) AR, rhBTC, recombinant mouse Epigen, and rhEpiregulin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
HBEGF
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 0.2-0.8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human HB-EGF Antibody (Catalog # AF-259-NA)
ELISA Detection: Human HB-EGF Biotinylated Antibody (Catalog # BAF259)
Standard:Recombinant Human HB-EGF (Catalog # 259-HE)
Readout System
Publications
Read Publications using
BAF259 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for HB-EGF Antibody [Biotin]

  • diphtheria toxin receptor (heparin-binding epidermal growth factor-like growthfactor)
  • Dtr
  • DTRHEGFLdiphtheria toxin receptor (heparin-binding EGF-like growth factor)
  • Dts
  • DTSF
  • HBEGF
  • HB-EGF
  • Hegfl
  • heparin-binding EGF-like growth factor
  • heparin-binding epidermal growth factor
  • proheparin-binding EGF-like growth factor

Background

HB-EGF was originally purified based on its heparin-binding property and mitogenic activity on BALB-3T3 fibroblasts from the conditioned medium of the human U-937 histiocytic lymphoma cell line. The natural protein has an apparent molecular mass of 19 - 23 kDa and exists in multiple forms as a result of heterogenous O‑glycosylation and/or N-terminal truncation. In addition to fibroblasts, HB-EGF is also a potent mitogen for keratinocytes and smooth muscle cells but not for capillary endothelial cells. HB-EGF is produced in monocytes and macrophages. In addition, transcription of HB-EGF can be induced in vascular endothelial cells as well as aortic smooth muscle cells (SMC), suggesting that HB-EGF may have an important role in the pathogenesis of atherosclerosis.

HB-EGF is a member of the EGF family of mitogens which also include transforming growth factor-alpha (TGF-alpha ), amphiregulin (AR), rat schwanoma-derived growth factor (SDGF), vaccinia growth factor (VGF), and the various ligands for the HER2/erbB2/neu receptor. All these cytokines are derived from transmembrane precursors that contain one or several EGF structural units in their extracellular domain. Many of these transmembrane precursors are biologically active and seem to play a role in juxtacrine stimulation of adjacent cells. The cDNA for HB-EGF encodes a 204 amino acid residue transmembrane protein that is proteolytically cleaved to generate the soluble HB-EGF. Like EGF, TGF-alpha , and AR; HB-EGF binds to the EGF receptor and activates the receptor tyrosine kinase. HB-EGF is reported to be a more potent SMC mitogen than EGF. It has been suggested that the differential activities found for HB-EGF compared to EGF may be mediated by the heparin-binding properties of HB-EGF. A diphtheria toxin receptor that mediates the endocytosis of the bound toxin has been cloned and found to be identical to the transmembrane HB-EGF precursor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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HB-EGF Antibody
[Biotin]
BAF259
HB-EGF Antibody [Biotin]
Species: Hu
Applications: WB, ELISA(Det)

Publications for HB-EGF Antibody (BAF259)(8)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: ELISA Development, ELISA Development (Detection), ELISA Microarray Development, Flow Cytometry.


Filter By Application
ELISA Development
(1)
ELISA Development (Detection)
(1)
ELISA Microarray Development
(1)
Flow Cytometry
(3)
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Filter By Species
Human
(3)
Mouse
(3)
All Species
Showing Publications 1 - 8 of 8.
Publications using BAF259 Applications Species
Chen-Hao Yeh, Joel Finney, Takaharu Okada, Tomohiro Kurosaki, Garnett Kelsoe Primary germinal center-resident T follicular helper cells are a physiologically distinct subset of CXCR5hiPD-1hi T follicular helper cells Immunity 2/1/2022 [PMID: 35081372]
Matthew B. Buechler, Ki-Wook Kim, Emily J. Onufer, Jesse W. Williams, Christine C. Little, Claudia X. Dominguez et al. A stromal niche defined by expression of the transcription factor Wilms tumor 1 mediates programming and homeostasis of cavity-resident macrophages Immunity 7/1/2019 [PMID: 31231034]
van Blijswijk J, Schraml B, Rogers N, Whitney P, Zelenay S, Acton S, Reis E Sousa C Altered lymph node composition in diphtheria toxin receptor-based mouse models to ablate dendritic cells. J Immunol, 2014-11-19;194(1):307-15. 2014-11-19 [PMID: 25411201] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Cole C, Rushton G, Jayson G, Avizienyte E Ovarian cancer cell heparan sulfate 6-O-sulfotransferases regulate an angiogenic program induced by heparin-binding epidermal growth factor (EGF)-like growth factor/EGF receptor signaling. J Biol Chem, 2014-02-22;289(15):10488-501. 2014-02-22 [PMID: 24563483] (ELISA Development (Detection), Human) ELISA Development (Detection) Human
Schmitt E, Haribhai D, Jeschke J, Co D, Ziegelbauer J, Yan K, Iwakura Y, Mishra M, Simpson P, Salzman N, Williams C Chronic follicular bronchiolitis requires antigen-specific regulatory T cell control to prevent fatal disease progression. J Immunol, 2013-10-25;191(11):5460-76. 2013-10-25 [PMID: 24163409] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Gonzalez RM, Seurynck-Servoss SL, Crowley SA Development and validation of sandwich ELISA microarrays with minimal assay interference. J. Proteome Res., 2008-04-19;7(6):2406-14. 2008-04-19 [PMID: 18422355] (ELISA Microarray Development, Human) ELISA Microarray Development Human
Kim JM, Rasmussen JP, Rudensky AY Regulatory T cells prevent catastrophic autoimmunity throughout the lifespan of mice. Nat. Immunol., 2006-11-30;8(2):191-7. 2006-11-30 [PMID: 17136045] (Flow Cytometry, Mouse) Flow Cytometry Mouse
Janes KA, Gaudet S, Albeck JG, Nielsen UB, Lauffenburger DA, Sorger PK The response of human epithelial cells to TNF involves an inducible autocrine cascade. Cell, 2006-03-24;124(6):1225-39. 2006-03-24 [PMID: 16564013] (ELISA Development, Human) ELISA Development Human

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Bioinformatics

Gene Symbol HBEGF
Uniprot