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Recombinant Human TGF-alpha Protein, CF

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human TGF-alpha Protein, CF Summary

Details of Functionality
Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. Marquardt, H. <EM>et al</EM>. (1984) Science <STRONG>223</STRONG>:1079.<br />The ED<SUB>50</SUB> for this effect is 0.1-0.4 ng/mL.<br /><br />
Accession #
N-terminal Sequence
Val40
Protein/Peptide Type
Recombinant Proteins
Gene
TGFA
Endotoxin Note
<0.010 EU per 1 µg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
239-A in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile 10 mM Acetic Acid.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human TGF-alpha Protein, CF

  • protransforming growth factor alpha
  • TFGA
  • TGFA
  • TGFalpha
  • TGF-alpha
  • transforming growth factor, alpha
  • transforming growth factor-alpha

Background

TGF-alpha was originally isolated from the conditioned media of oncogenically transformed cells as an EGF-like bioactivity. TGF-alpha is a member of the EGF family of cytokines that are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released from the transmembrane protein by proteolytic cleavage. Membrane-bound proTGF-alpha is biologically active and seems to play a role in mediation of cell-cell adhesion and in juxtacrine stimulation of adjacent cells. Expression of TGF-alpha is widespread in tumors and transformed cells. TGF-alpha is also expressed in normal tissues during embryogenesis and in adult tissues, including pituitary, brain, keratinocytes and macrophages. Mature TGF-alpha  shows approximately 93% amino acid sequence identity with mouse or rat TGF-alpha and is not species specific in its biological effects.

TGF-alpha binds to the EGF receptor and activates the receptor tyrosine kinase. Accordingly, TGF-alpha shows a similar potency to EGF as a mitogen for fibroblasts and as an inducer of epithelial development in vivo. TGF-alpha is reportedly more potent than EGF as an angiogenic factor in vivo and as a stimulator for keratinocyte migration. The EGF receptor gene represents the cellular homologue of the avian v-erb-B oncogene.

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239-A
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Applications: Bioactivity

Publications for TGF-alpha (239-A)(58)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Avian - Chicken, Chicken, Chinese Hamster, Hamster, Porcine.

We have publications tested in 6 applications: Bioassay, Cell Culture, ELISA (Standard), ELISA Developmet, In Vivo, Stimulation.


Filter By Application
Bioassay
(48)
Cell Culture
(1)
ELISA (Standard)
(1)
ELISA Developmet
(1)
In Vivo
(4)
Stimulation
(2)
All Applications
Filter By Species
Human
(38)
Mouse
(11)
Rat
(3)
Avian - Chicken
(1)
Chicken
(1)
Chinese Hamster
(1)
Hamster
(1)
Porcine
(1)
All Species
Showing Publications 1 - 10 of 58. Show All 58 Publications.
Publications using 239-A Applications Species
Wirth, D;Özdemir, E;Hristova, K; Quantification of ligand and mutation-induced bias in EGFR phosphorylation in direct response to ligand binding Nature communications 2023-11-21 [PMID: 37989743] (Bioassay, Chinese Hamster) Bioassay Chinese Hamster
Kamentseva, RS;Kharchenko, MV;Gabdrahmanova, GV;Kotov, MA;Kosheverova, VV;Kornilova, ES; EGF, TGF-? and Amphiregulin Differently Regulate Endometrium-Derived Mesenchymal Stromal/Stem Cells International journal of molecular sciences 2023-08-29 [PMID: 37686213] (Bioassay, Human) Bioassay Human
VanSlyke, JK;Boswell, BA;Musil, LS; ErbBs in Lens Cell Fibrosis and Secondary Cataract Investigative ophthalmology & visual science 2023-07-03 [PMID: 37418274] (Bioassay, Avian - Chicken) Bioassay Avian - Chicken
Takada, H;Sasagawa, Y;Yoshimura, M;Tanaka, K;Iwayama, Y;Hayashi, T;Isomura-Matoba, A;Nikaido, I;Kurisaki, A; Single-cell transcriptomics uncovers EGFR signaling-mediated gastric progenitor cell differentiation in stomach homeostasis Nature communications 2023-06-29 [PMID: 37386010] (Bioassay, Mouse) Bioassay Mouse
Y Wang, Q Zheng, Z Sun, C Wang, J Cen, X Zhang, Y Jin, B Wu, T Yan, Z Wang, Q Gu, X Lv, J Nan, Z Wu, W Sun, G Pan, L Zhang, L Hui, X Cai Reversal of liver failure using a bioartificial liver device implanted with clinical-grade human-induced hepatocytes Cell Stem Cell, 2023-04-13;0(0):. 2023-04-13 [PMID: 37059100] (Human) Human
JE Kaserman, RB Werder, F Wang, T Matte, MI Higgins, M Dodge, J Lindstrom-, P Bawa, A Hinds, E Bullitt, IS Caballero, X Shi, RE Gerszten, N Brunetti-P, M Liesa, C Villacorta, AN Hollenberg, DN Kotton, AA Wilson Human iPSC-hepatocyte modeling of alpha-1 antitrypsin heterozygosity reveals metabolic dysregulation and cellular heterogeneity Cell Reports, 2022-12-06;41(10):111775. 2022-12-06 [PMID: 36476855] (Bioassay, Human) Bioassay Human
S Gnosa, L Puig-Blasc, KB Piotrowski, ML Freiberg, S Savickas, DH Madsen, U Auf dem Ke, P Kronqvist, M Kveiborg ADAM17-mediated EGFR ligand shedding directs macrophage promoted cancer cell invasion JCI Insight, 2022-09-22;0(0):. 2022-09-22 [PMID: 35998057] (Stimulation, Human) Stimulation Human
C Monteiro, L Miarka, M Perea-Garc, N Priego, P García-Góm, L Álvaro-Esp, A de Pablos-, N Yebra, D Retana, P Baena, C Fustero-To, O Graña-Cast, K Troulé, E Caleiras, P Tezanos, P Muela, E Cintado, JL Trejo, JM Sepúlveda, P González-L, L Jiménez-Ro, LM Moreno, O Esteban, Á Pérez-Núñe, A Hernández-, J Mazarico G, I Ferrer, R Suárez, EM Garrido-Ma, L Paz-Ares, C Dalmasso, E Cohen-Jona, A Siegfried, A Hegarty, S Keelan, D Varešlija, LS Young, M Mohme, Y Goy, H Wikman, J Fernández-, G Blasco, L Alcázar, C Cabañuz, SI Grivenniko, A Ianus, N Shemesh, CC Faria, R Lee, P Lorigan, E Le Rhun, M Weller, R Soffietti, L Bertero, U Ricardi, J Bosch-Barr, E Sais, E Teixidor, A Hernández-, A Calvo, J Aristu, SM Martin, A Gonzalez, O Adler, N Erez, RENACER, M Valiente Stratification of radiosensitive brain metastases based on an actionable S100A9/RAGE resistance mechanism Nature Medicine, 2022-04-11;28(4):752-765. 2022-04-11 [PMID: 35411077] (Bioassay, Human) Bioassay Human
C Hu, CA Leche, A Kiyatkin, Z Yu, SE Stayrook, KM Ferguson, MA Lemmon Glioblastoma mutations alter EGFR dimer structure to prevent ligand bias Nature, 2022-02-09;602(7897):518-522. 2022-02-09 [PMID: 35140400] (Bioassay, Human) Bioassay Human
S Steiner, GM Seleznik, T Reding, M Stopic, D Lenggenhag, E Ten Buren, D Eshmuminov, K Endhardt, C Hagedorn, AM Heidenblut, A Bratus-Neu, J Grossmann, C Trachsel, KS Jabbar, SA Hahn, JV Berg, R Graf, A Gupta De novo expression of gastrokines in pancreatic precursor lesions impede the development of pancreatic cancer Oncogene, 2022-01-26;0(0):. 2022-01-26 [PMID: 35082384] (Bioassay, Mouse) Bioassay Mouse
Show All 58 Publications.

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Bioinformatics

Gene Symbol TGFA
Uniprot