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Haptoglobin Antibody

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Western blot shows samples of purified mouse Haptoglobin (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4409) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band for Haptoglobin was detected at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Western blot shows samples of purified mouse Haptoglobin (5 ng/lane). PVDF membrane was probed with 1 µg/mL Sheep Anti-Mouse Haptoglobin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4409) followed by ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF4409-SP
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Haptoglobin Antibody Summary

Immunogen
Mouse serum-derived Haptoglobin
Specificity
Detects mouse Haptoglobin in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with human Haptoglobin is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
HP
Purity Statement
Antigen Affinity-purified
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Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF4409 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Haptoglobin Antibody

  • binding peptide
  • BP
  • haptoglobin alpha(1S)-beta
  • haptoglobin alpha(2FS)-beta
  • Haptoglobin
  • haptoglobin, alpha polypeptide
  • haptoglobin, beta polypeptide
  • HP
  • HP-1
  • hp2-alpha
  • HP2ALPHA2
  • HPA1S
  • MGC111141
  • zonulin

Background

Haptoglobin is a 45‑48 kDa acute phase glycoprotein that is a naturally inactive member of the peptidase S1 family of serine proteases. It is secreted by multiple cell types and protects tissues from hemoglobin-mediated oxidative damage. Mature mouse (pro)haptoglobin is 329 amino acids (aa) in length. Proteolytic cleavage between Arg102-Ile103 creates disulfide-linked alpha - and beta -chains, which subsequently homodimerize through alpha -chain linkages to create a tetramer. Circulating haptoglobin is 70% tetramer and 30% unprocessed (pro)haptoglobin. Mouse (pro)haptoglobin shares 90% and 80% aa identity with rat and human (pro)haptoglobin, respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Haptoglobin Antibody (AF4409)(3)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: Western Blot.


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Secondary Antibodies

 

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Bioinformatics

Gene Symbol HP