HAP1 Antibody (1B6) - Azide and BSA Free Summary
Immunogen |
Fusion protein from rat HAP1. [UniProt# P54256] |
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
HAP1 |
Purity |
Protein G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 1 ug per million cells
- Immunocytochemistry/ Immunofluorescence reported in scientific literature
- Immunohistochemistry 0.5-1.0 ug/ml
- Immunohistochemistry-Frozen 0.5-1.0 ug/ml
- Immunohistochemistry-Paraffin 0.5-1.0 ug/ml
- Immunoprecipitation 1:10-1:500
- Western Blot 0.5-1.0 ug/ml
|
Application Notes |
In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B. |
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
PBS and 50% Glycerol |
Preservative |
No Preservative |
Concentration |
1 mg/ml |
Purity |
Protein G purified |
Alternate Names for HAP1 Antibody (1B6) - Azide and BSA Free
Background
HAP1 (Huntingtin-associated protein 1) is a cytoplasmic protein that interacts with several proteins such as Huntingtin, p150Glued, KLC, TrkA, TATA-binding protein, proBDNF etc. and plays a key role in microtubule-dependent transport, vesicular trafficking, nerve growth, transcriptional regulation and signal transduction. HAP1 was originally discovered as huntingtin interacting neuronal protein (htt), the Huntington's disease protein, however, unlike htt (expressed ubiquitously throughout body), HAP1 is enriched in brain with abundant expression in the hypothalamus, amygdala, brain stem etc. HAP1 has been associated with microtubule-dependent transporters dynactin p150 and kinesin light chain, as well as with transport of various vesicular proteins or membrane receptors, including BDNF-containing vesicles, EGFR, type 1 inositol (1,4,5)-triphosphate receptor, gamma -aminobutyric acid, type A, receptor, and TRKA. Hap1 is also implicated in microtubule-dependent trafficking in neurons, vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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