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HAP1 Antibody (1B6) - Azide and BSA Free

Images

 
Western Blot: HAP1 Antibody (1B6) - Azide and BSA Free [NBP2-80758] - Detection of HAP1 in mouse brain lysate. Image from the standard format of this antibody.
Immunohistochemistry: HAP1 Antibody (1B6) - Azide and BSA Free [NBP2-80758] - Staining of rat hypothalamus. Image from the standard format of this antibody.
Flow Cytometry: HAP1 Antibody (1B6) - Azide and BSA Free [NBP2-80758] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using HAP1 antibody (dark blue). Isotype control shown in orange. An antibody ...read more
Western Blot: HAP1 Antibody (1B6) - Azide and BSA Free [NBP2-80758] - Analysis of HAP1 using Lane 1: mouse brain lysate and Lane 2: human brain lysate. Image from the standard format of this antibody.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IP
Clone
1B6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

Order Details

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HAP1 Antibody (1B6) - Azide and BSA Free Summary

Immunogen
Fusion protein from rat HAP1. [UniProt# P54256]
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
HAP1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 1 ug per million cells
  • Immunocytochemistry/ Immunofluorescence reported in scientific literature
  • Immunohistochemistry 0.5-1.0 ug/ml
  • Immunohistochemistry-Frozen 0.5-1.0 ug/ml
  • Immunohistochemistry-Paraffin 0.5-1.0 ug/ml
  • Immunoprecipitation 1:10-1:500
  • Western Blot 0.5-1.0 ug/ml
Application Notes
In Western blot bands can be seen ~95 kDa for isoform A and ~110 kDa for isoform B.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 50% Glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for HAP1 Antibody (1B6) - Azide and BSA Free

  • HAP2huntingtin-associated protein 2
  • hHLP1
  • HIP5
  • HLP1
  • HLPHAP-1
  • huntingtin-associated protein 1
  • Neuroan 1

Background

HAP1 (Huntingtin-associated protein 1) is a cytoplasmic protein that interacts with several proteins such as Huntingtin, p150Glued, KLC, TrkA, TATA-binding protein, proBDNF etc. and plays a key role in microtubule-dependent transport, vesicular trafficking, nerve growth, transcriptional regulation and signal transduction. HAP1 was originally discovered as huntingtin interacting neuronal protein (htt), the Huntington's disease protein, however, unlike htt (expressed ubiquitously throughout body), HAP1 is enriched in brain with abundant expression in the hypothalamus, amygdala, brain stem etc. HAP1 has been associated with microtubule-dependent transporters dynactin p150 and kinesin light chain, as well as with transport of various vesicular proteins or membrane receptors, including BDNF-containing vesicles, EGFR, type 1 inositol (1,4,5)-triphosphate receptor, gamma -aminobutyric acid, type A, receptor, and TRKA. Hap1 is also implicated in microtubule-dependent trafficking in neurons, vesicular transport, gene transcription regulation, membrane receptor trafficking and other functions such as calcium release and protein aggregation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for HAP1 Antibody (NBP2-80758) (0)

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Secondary Antibodies

 

Isotype Controls

Additional HAP1 Products

Array NBP2-80758

Research Areas for HAP1 Antibody (NBP2-80758)

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Bioinformatics

Gene Symbol HAP1