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GULP1/CED-6 Antibody

Images

 
Orthogonal Strategies: Immunohistochemistry-Paraffin: GULP1/CED-6 Antibody [NBP1-84553] - Staining in human smooth muscle and tonsil tissues using anti-GULP1 antibody. Corresponding GULP1 RNA-seq data are ...read more
Western Blot: GULP1/CED-6 Antibody [NBP1-84553] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells).
Immunocytochemistry/ Immunofluorescence: GULP1/CED-6 Antibody [NBP1-84553] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: GULP1/CED-6 Antibody [NBP1-84553] - Staining of human smooth muscle shows high expression.
Immunohistochemistry-Paraffin: GULP1/CED-6 Antibody [NBP1-84553] - Staining of human tonsil shows low expression as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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GULP1/CED-6 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: KTDKRIFTFICKDSESNKHLCYVFDSEKCAEEITLTIGQAFDLAYRKFLESGGKDVETRKQIAGLQKRIQDLETENMELKNKVQDLENQLRITQVSA
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GULP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
GULP1/CED-6 Protein (NBP1-84553PEP)
Publications
Read Publications using
NBP1-84553 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for GULP1/CED-6 Antibody

  • CED6
  • CED6GULPCED-6
  • Cell death protein 6 homolog
  • engulfment adapter protein
  • FLJ31156
  • GULP, engulfment adaptor PTB domain containing 1
  • GULP1
  • Protein GULP
  • PTB domain adapter protein CED-6
  • PTB domain adaptor protein CED-6
  • PTB domain-containing engulfment adapter protein 1

Background

The prompt clearance of cells undergoing apoptosis is critical during embryonic development, normal tissue turnover, as well as inflammation and autoimmunity. CED6 and its human homologue gulp, encode an adapter protein, whose function in engulfment. The upstream and downstream components of CED6 mediated signaling are not known. Recently, CED1 has been shown to encode a transmembrane protein on phagocytic cells, with two functional sequence motifs in its cytoplasmic tail that are important for engulfment.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GULP1/CED-6 Antibody (NBP1-84553)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GULP1/CED-6 Antibody (NBP1-84553). (Showing 1 - 1 of 1 FAQs).

  1. Is GULP1 localized to mitochondria?
    • I am showing based on localization data from UniProt that it may associate with the cytoplasmic side of the plasma membrane: http://www.uniprot.org/uniprot/Q9UBP9 Additional localization data may be found here at Human protein atlas: http://www.proteinatlas.org/ENSG00000144366

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GULP1