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GSK-3 beta [p Ser9] Antibody (609739)

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes. PVDF Membrane was probed with 1 µg/mL of Human Phospho-GSK-3 beta (S9) Monoclonal Antibody (Catalog # MAB25062) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Phospho-GSK-3 beta (S9) at approximately 46 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line untreated (-) or treated (+) with 200 nM PMA for 20 minutes. PVDF Membrane was probed with 1 µg/mL of Human Phospho-GSK-3 beta (S9) ...read more
GSK-3 beta phosphorylated at S9 was detected in immersion fixed HeLa human cervical epithelial carcinoma cells, unstimulated (lower panel) or stimulated (upper panel) with PMA, using Mouse Anti-Human Phospho-GSK-3 beta ...read more
HeLa human cervical epithelial carcinoma cell line untreated (open histogram) or treated with 120 ng/mL PMA for 15 minutes (filled histogram) was stained with Human Phospho-GSK‑3 beta (S9) Monoclonal Antibody ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow, ICC/IF
Clone
609739
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
IC25062U-100UG
Alexa Fluor 405
IC25062V-100UG
Alexa Fluor 488
IC25062G-100UG
Alexa Fluor 532
FAB25062X
Alexa Fluor 594
IC25062T-100UG
Alexa Fluor 647
IC25062R-100UG
Alexa Fluor 700
IC25062N-100UG
Alexa Fluor 750
IC25062S-100UG
Allophycocyanin
FAB25062A
Biotin
FAB25062B
CoraFluor 1
FAB25062CL1
DyLight 350
FAB25062D
DyLight 405
FAB25062E
DyLight 488
FAB25062K
DyLight 550
FAB25062L
DyLight 594
FAB25062M
DyLight 650
FAB25062W
DyLight 680
FAB25062Y
DyLight 755
FAB25062Z
HRP
FAB25062H
Janelia Fluor 525
FAB25062JF525
Janelia Fluor 549
FAB25062I
Janelia Fluor 646
FAB25062J
Janelia Fluor 669
FAB25062JF669
PE
FAB25062P
PerCP
FAB25062C
mFluor Violet 450 SE
FAB25062MFV450
mFluor Violet 500 SE
FAB25062MFV500
mFluor Violet 610 SE
FAB25062MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB25062-SP
Carrier Free
IC25062V-100UG
Carrier Free
IC25062U-100UG
Carrier Free
IC25062T-100UG
Carrier Free
IC25062S-100UG
Carrier Free
IC25062R-100UG
Carrier Free
IC25062N-100UG
Carrier Free
IC25062G-100UG
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GSK-3 beta [p Ser9] Antibody (609739) Summary

Immunogen
Phosphopeptide containing the human GSK-3 beta S9 site
Modification
p Ser9
Specificity
Detects human GSK-3 beta when phosphorylated at S9.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GSK3B
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSK-3 beta [p Ser9] Antibody (609739)

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified asan inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta , inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol GSK3B