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GSK-3 beta Antibody (3D10) [Allophycocyanin/Cy7]

 
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Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, Simple Western, ELISA, Flow, ICC/IF, IHC
Clone
3D10
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin/Cy7

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-47470AF350
Alexa Fluor 405
NBP1-47470AF405
Alexa Fluor 488
NBP1-47470AF488
Alexa Fluor 532
NBP1-47470AF532
Alexa Fluor 594
NBP1-47470AF594
Alexa Fluor 647
NBP1-47470AF647
Alexa Fluor 700
NBP1-47470AF700
Alexa Fluor 750
NBP1-47470AF750
Allophycocyanin
NBP1-47470APC
Biotin
NBP1-47470B
CoraFluor 1
NBP1-47470CL1
DyLight 350
NBP1-47470UV
DyLight 405
NBP1-47470V
DyLight 488
NBP1-47470G
DyLight 550
NBP1-47470R
DyLight 594
NBP1-47470DL594
DyLight 650
NBP1-47470C
DyLight 680
NBP1-47470FR
DyLight 755
NBP1-47470IR
FITC
NBP1-47470F
HRP
NBP1-47470H
Janelia Fluor 549
NBP1-47470JF549
Janelia Fluor 646
NBP1-47470JF646
PE
NBP1-47470PE
PE/Atto594
NBP1-47470PEATT594
PE/Cy5.5
NBP1-47470PECY55
PE/Cy7
NBP1-47470PECY7
PerCP
NBP1-47470PCP
Unconjugated
NBP1-47470
mFluor Violet 450 SE
NBP1-47470MFV450
mFluor Violet 500 SE
NBP1-47470MFV500
mFluor Violet 610 SE
NBP1-47470MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
NBP1-47470
BSA Free
NBP1-47470
BSA Free
NBP1-47470SS
Azide and BSA Free
NBP2-80757
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GSK-3 beta Antibody (3D10) [Allophycocyanin/Cy7] Summary

Immunogen
Purified recombinant fragment of human GSK3 beta expressed in E. coli. [UniProt# P49841]
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
GSK3B
Purity
Ammonium sulfate precipitation
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Applications/Dilutions

Dilutions
  • ELISA
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Simple Western
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Ammonium sulfate precipitation

Alternate Names for GSK-3 beta Antibody (3D10) [Allophycocyanin/Cy7]

  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 beta
  • glycogen synthase kinase-3 beta
  • GSK3 beta
  • GSK-3 beta
  • GSK3B
  • GSK3beta isoform

Background

Glycogen synthase kinase 3beta (GSK-3 beta) is a Serine/Threonine protein kinas involved in the insulin and wingless pathways (1-2). Originally identified as a regulator of glycogen synthase, it has been shown to regulate a diverse array of cellular functions. GSK-3 beta activity is down regulated by phosphorylation on Serine 9 by PKB, or activated by phosphorylation on Tyrosine 216. Once activated, the protein can phosphorylate p53, c-jun, heat shock factor-1 and cyclin D1 (3-4). It is also known to phosphorylate Tau in Allzheimer's disease. Additionally, increased GSK-3 beta protein levels are found in Alzheimer's disease brains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GSK3B