![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0013.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
| Reactivity | Hu, Mu, RtSpecies Glossary |
| Applications | WB, ICC/IF, IHC, IP |
| Clone | SY26-05 |
| Clonality | Monoclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Concentration | 1 mg/ml |
| Additional Information | Recombinant Monoclonal Antibody. |
| Immunogen | Synthetic phospho-peptide corresponding to residues surrounding Tyr216 and 279 of human GSK-3 alpha/beta (SwissProt: P49840 Human; SwissProt: P49841 Human; SwissProt: Q2NL51 Mouse; SwissProt: Q9WV60 Mouse; SwissProt: P18265 Rat; SwissProt: P18266 Rat) |
| Modification | p Tyr216, p Tyr279 |
| Localization | Cytoplasm, Nucleus, Cell membrane. |
| Isotype | IgG |
| Clonality | Monoclonal |
| Host | Rabbit |
| Gene | GSK3A |
| Purity | Protein A purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | TBS (pH7.4), 0.05% BSA, 40% Glycerol |
| Preservative | 0.05% Sodium Azide |
| Concentration | 1 mg/ml |
| Purity | Protein A purified |
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![Western Blot beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/beta-Catenin_AF1329_Western_Blot_5100.jpg)
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![Simple Western beta-Catenin Antibody [Unconjugated]](https://images.novusbio.com/images2/betaCatenin_AF1329_Simple_Western_17134.jpg)
![GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05)](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05)")
| Publication using NBP2-67441 | Applications | Species |
|---|---|---|
| Shandilya S, Kesari KK,Ruokolainen J Vitamin K2 Modulates Organelle Damage and Tauopathy Induced by Streptozotocin and Menadione in SH-SY5Y Cells Antioxidants (Basel, Switzerland) 2021-06-20 [PMID: 34202933] (ICC/IF, Human) | ICC/IF | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for GSK-3 alpha/beta Antibody (NBP2-67441)Find related products by research area.
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| Gene Symbol | GSK3A |
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0003.jpg "Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0017.jpg "Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on mouse cerebellum tissue lysates. Lane 1: mouse cerebellum tissue, whole cell lysate, 20ug/laneLane 2: mouse cerebellum tissue treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 20ug/lane All lanes : Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0010.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on mouse cerebellum tissue lysates. Lane 1: mouse cerebellum tissue, whole cell lysate, 20ug/laneLane 2: mouse cerebellum tissue treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 20ug/lane All lanes : Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes")
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on 293T cell lysates.Lane 1: 293T cells, whole cell lysate, 10ug/lane Lane 2: 293T cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes :Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0011.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on 293T cell lysates.Lane 1: 293T cells, whole cell lysate, 10ug/lane Lane 2: 293T cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes :Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes")
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: A431 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0012.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: A431 cell lysate Lane 2: Hela cell lysate")
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0001.jpg "Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0002.jpg "Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.")
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0004.jpg "Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0005.jpg "Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0006.jpg "Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.")
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-GSK-3 alpha/beta (p Tyr216+Y279) antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0014.jpg "Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-GSK-3 alpha/beta (p Tyr216+Y279) antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0015.jpg "Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279] antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0016.jpg "Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279] antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.")
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![Immunocytochemistry Insulin Antibody (182410) [Unconjugated]](https://images.novusbio.com/images2/Insulin_MAB1417_Immunocytochemistry_9376.jpg)
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![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")