GSK-3 alpha/beta Primary Antibodies
We offer GSK-3 alpha/beta Antibodies for use in common research applications: ELISA, Flow (Intracellular), Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot. Each GSK-3 alpha/beta Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when you need it. Our GSK-3 alpha/beta Antibodies can be used in a variety of model species: Bovine, Canine, Human, Mouse, Porcine, Primate, Rabbit, Rat, Sheep. Use the list below to choose the GSK-3 alpha/beta Antibody which is most appropriate for your research; you can click on each one to view full technical details, images, references, reviews and related products. Choose from our GSK-3 alpha/beta polyclonal antibodies and browse our GSK-3 alpha/beta monoclonal antibody catalog.
Alternate Names for GSK-3 alpha/beta Primary Antibodies
anti-GSK-3 alpha/beta antibody, anti-GSK3A antibody, anti-DKFZp686D0638 antibody, anti-EC 2.7.11 antibody, anti-EC 2.7.11.26 antibody, anti-glycogen synthase kinase 3 alpha antibody, anti-glycogen synthase kinase-3 alpha antibody, anti-GSK-3 alpha antibody
11 Results for "gsk-3-alpha-beta" in Primary Antibodies
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0013.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of GSK-3 alpha/beta [p Tyr216, p Tyr279] on mouse brain tissue lysates with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279] antibody at 1:500 dilution. Lysates/proteins at 20 ug/Lane. Predicted band size: 46/51 kDa Observed band size: 46/51 kDa Exposure time: 2 minutes; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1:500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:300,000 dilution was used for 1 hour at room temperature.")
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0003.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human thyroid carcinoma tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279) antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/800 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0017.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on mouse cerebellum tissue lysates. Lane 1: mouse cerebellum tissue, whole cell lysate, 20ug/laneLane 2: mouse cerebellum tissue treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 20ug/lane All lanes : Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0010.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on 293T cell lysates.Lane 1: 293T cells, whole cell lysate, 10ug/lane Lane 2: 293T cells treated with 2.8ug/ul lambda-PP for 30 minutes, whole cell lysates, 10ug/lane All lanes :Anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody at 1:500 dilution. Anti-GAPDH antibody at 1:10,000 dilution. Goat Anti-Rabbit IgG H&L (HRP) at 1/200,000 dilution. Predicted band size: 47/51 kDaObserved band size: 47/51 kDaBlocking and diluting buffer: 5% BSA.Exposure time: 2 minutes](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0011.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of Phospho-GSK3(alpha+beta)(Y216+Y279) on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: A431 cell lysate Lane 2: Hela cell lysate](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Western-Blot-NBP2-67441-img0012.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0001.jpg)
![Immunocytochemistry/Immunofluorescence: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Staining Phospho-GSK3(alpha+beta)(Y216+Y279) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunocytochemistry-Immunofluorescence-NBP2-67441-img0002.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0004.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0005.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue using anti-Phospho-GSK3(alpha+beta)(Y216+Y279) antibody. Counter stained with hematoxylin.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0006.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-Phospho-GSK-3 alpha/beta (p Tyr216+Y279) antibody000 dilution. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0014.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded rat hippocampus tissue with Rabbit anti-GSK-3 alpha/beta [p Tyr216, p Tyr279) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0015.jpg)
![Immunohistochemistry-Paraffin: GSK-3 alpha/beta [p Tyr216, p Tyr279] Antibody (SY26-05) [NBP2-67441] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-Phospho-GSK3 alpha/beta [p Tyr216, p Tyr279] antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-p-Tyr216-p-Tyr279-Antibody-SY26-05-Immunohistochemistry-Paraffin-NBP2-67441-img0016.jpg)
![Western Blot: GSK-3 alpha/beta Antibody (1H8) [NB110-85523] - Analysis: Lane 1: MW marker, Lane 2: 20ug HeLa lysate (heat shocked), Lane 3: 20ug mouse brain lysate, Lane 4: 20ug rat brain lysate, Lane 5: 20ug RK-13 lysate.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-Antibody-1H8-Western-Blot-NB110-85523-img0003.jpg "Western Blot: GSK-3 alpha/beta Antibody (1H8) [NB110-85523] - Analysis: Lane 1: MW marker, Lane 2: 20ug HeLa lysate (heat shocked), Lane 3: 20ug mouse brain lysate, Lane 4: 20ug rat brain lysate, Lane 5: 20ug RK-13 lysate.")
![Immunohistochemistry-Paraffin: GSK3 alpha/beta Antibody (1H8) [NB110-85523] - Alzgeuner diseased neurons with granulovacuolar degeneration co-stained with GSK alpha/beta Monoclonal Antibody (1H8) (Brown Precipitate).](http://images.novusbio.com/fullsize/GSK3-alpha-beta-Antibody-1H8-Immunohistochemistry-Paraffin-NB110-85523-img0002.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr 216] Antibody (SR1908) [NBP3-21575] - Western blot analysis using NBP3-21575 in 293 cell lysate treated with AP.](http://images.novusbio.com/fullsize/nbp3-21575_rabbit-gsk-3-alpha-beta-p-tyr-216-mab-sr1908-57202316494258.jpg "Western Blot: GSK-3 alpha/beta [p Tyr 216] Antibody (SR1908) [NBP3-21575] - Western blot analysis using NBP3-21575 in 293 cell lysate treated with AP.")
![Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution or GSK3alpha antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Western-Blot-NBP3-05443-img0003.jpg "Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution or GSK3alpha antibody (A0645).293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.")
![Western Blot: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Western blot analysis of extracts of 293 and HeLa cells, using GSK-3 alpha/beta antibody (NBP3-05443) at 1:1000 dilution. 293 cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. HeLa cells were treated by Insulin (0.01U/mL) for 15 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Western-Blot-NBP3-05443-img0002.jpg)
![Immunoprecipitation: GSK-3 alpha/beta [p Ser21] Antibody [NBP3-05443] - Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug GSK-3 alpha/beta pAb (NBP3-05443). Western blot was performed from the immunoprecipitate using GSK-3 alpha/beta pAb (NBP3-05443) at a dilution of 1:1000. 293T cells were treated by Insulin (100 nM) at 37C for 10 minutes after serum-starvation overnight.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-[p-Ser21]-Antibody-Immunoprecipitation-NBP3-05443-img0001.jpg)
![Western Blot: GSK-3 alpha/beta [p Tyr216] Antibody (M132) [NBP3-23367] - Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3 beta (lanes 1 & 2) or anti-GSK-3 alpha/beta (Y279/Y216) (lanes 3 & 4).](http://images.novusbio.com/fullsize/nbp3-23367_mouse-gsk-3-alpha-beta-p-tyr216-mab-m132-19920231856920.jpg "Western Blot: GSK-3 alpha/beta [p Tyr216] Antibody (M132) [NBP3-23367] - Western blot analysis of rabbit spleen fibroblasts serum starved for 2 hrs (lanes 1 & 3) or treated with pervanadate (1 mM) for 30 minutes (lanes 2 & 4). The blot was probed with anti-GSK-3 beta (lanes 1 & 2) or anti-GSK-3 alpha/beta (Y279/Y216) (lanes 3 & 4).")
![Western Blot: GSK-3 alpha/beta Antibody (S08-8G7) [NBP3-19938] - Western blot detection of GSK-3 alpha/beta in Jurkat, Rat Brain, C6, CHO-K1, Hela cell lysates using NBP3-19938 (1:1000 diluted). Predicted band size: 51, 47 kDa. Observed band size: 51, 47kDa.](http://images.novusbio.com/fullsize/GSK-3-alpha-beta-Antibody-S08-8G7-Western-Blot-NBP3-19938-img0001.jpg "Western Blot: GSK-3 alpha/beta Antibody (S08-8G7) [NBP3-19938] - Western blot detection of GSK-3 alpha/beta in Jurkat, Rat Brain, C6, CHO-K1, Hela cell lysates using NBP3-19938 (1:1000 diluted). Predicted band size: 51, 47 kDa. Observed band size: 51, 47kDa.")
![Western Blot: GSK3 alpha/beta [p Ser9] Antibody [NB300-741] - Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3 beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).](http://images.novusbio.com/fullsize/GSK3-alpha-beta-[p-Ser9]-Antibody-Western-Blot-NB300-741-img0001.jpg "Western Blot: GSK3 alpha/beta [p Ser9] Antibody [NB300-741] - Western blot of rat cortex lysate showing specific immunolabeling of the ~46k GSK3 beta protein phosphorylated at Ser9 (control). Immunolabeling is blocked by the phospho-peptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).")
![Western Blot: GSK3 alpha/beta Antibody [NBP2-29873] - HEK293 cell lysate was probed with anti-GSK3alpha /Beta (1:5,000 dilution). Arrows indicate GSK3alpha (~51kDa) and GSK3Beta (~46kDa).](http://images.novusbio.com/fullsize/GSK3-alpha-beta-Antibody-Western-Blot-NBP2-29873-img0001.jpg "Western Blot: GSK3 alpha/beta Antibody [NBP2-29873] - HEK293 cell lysate was probed with anti-GSK3alpha /Beta (1:5,000 dilution). Arrows indicate GSK3alpha (~51kDa) and GSK3Beta (~46kDa).")