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GSK-3 alpha/beta Antibody (833916) [Unconjugated]

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Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for GSK-3a at approximately 46 and GSK-3 beta at approximately 51 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Western blot shows lysates of U2OS human osteosarcoma cell line and HeLa human cervical epithelial carcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody ...read more
GSK-3a/ beta was detected in immersion fixed HT-29 human colon adenocarcinoma cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells ...read more
GSK-3a/ beta was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using Mouse Anti-Human GSK-3a/ beta Monoclonal Antibody (Catalog # MAB25063) at 3 µg/mL for 3 hours at room temperature. Cells ...read more

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF
Clone
833916
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB25063-SP
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GSK-3 alpha/beta Antibody (833916) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GSK-3 beta
Asp200-Phe305
Accession # P49841
Specificity
Detects human GSK-3 beta in direct ELISAs and human GSK-3 alpha and GSK-3 beta in Western blots. Detects human and mouse GSK-3 alpha / beta in immunocytochemistry.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
GSK3A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunocytochemistry 3-25 ug/mL
  • Western Blot 2 ug/mL
Publications
Read Publications using
MAB25063 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GSK-3 alpha/beta Antibody (833916) [Unconjugated]

  • DKFZp686D0638
  • EC 2.7.11
  • EC 2.7.11.26
  • glycogen synthase kinase 3 alpha
  • glycogen synthase kinase-3 alpha
  • GSK-3 alpha
  • GSK-3 alpha/beta

Background

Glycogen Synthase Kinase-3 (GSK-3) is a serine/threonine kinase initially identified as an inhibitor of glycogen synthase. Two isoforms (GSK-3 alpha and GSK-3 beta ) share 85% amino acid identity. GSK-3 beta , inhibited by phosphorylation at S9 by Akt, is involved in energy metabolism, body pattern formation, and neuronal cell development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GSK-3 alpha/beta
Antibody (833916) [Un ...
MAB25063
GSK-3 alpha/beta Antibody (833916) [Unconjugated]
Species: Hu, Mu
Applications: WB, ICC/IF

Publications for GSK-3 alpha/beta Antibody (MAB25063)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Western Blot.


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Bioinformatics

Gene Symbol GSK3A
Uniprot