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GRK5 Antibody [Unconjugated]

Images

 
Western blot shows lysates of Ramos human Burkitt's lymphoma cell line, Rat-2 rat embryonic fibroblast cell line, and BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Human/Mouse/Rat GRK5 Antigen ...read more
Genetic Strategies: Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and GRK5 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH
Validated by:
     

Genetic Strategies

   

Order Details

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Catalog# & Formulation Size Price
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GRK5 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human GRK5
Pro463-Ser590
Accession # P34947
Specificity
Detects endogenous human, mouse, and rat GRK5 in Western blots.
Details of Functionality
None<br /><br />None<br /><br />
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GRK5
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Knockout Validated
  • Western Blot 0.5 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF4539 in the following applications:

Publications
Read Publications using
AF4539 in the following applications:

Packaging, Storage & Formulations

Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GRK5 Antibody [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.16
  • FLJ39780
  • G protein-coupled receptor kinase 5
  • G protein-coupled receptor kinase GRK5
  • GPRK5
  • GPRK5FP2025
  • GRK5

Background

G protein-coupled receptor kinases (GRKs) are important modulators of G protein-coupled receptor (GPCR) signaling. Receptor phosphorylation by specific GRKs plays a key role in triggering rapid desensitization. The GRK family consists of 7 isoforms that share a central catalytic domain with homology to other serine/threonine kinases. The catalytic domain is flanked by an amino-terminal RGS domain of 183‑188 amino acids and a carboxyl-terminus of variable length. GRK5 is a member of the GRK4 subfamily, and is expressed in a wide variety of tissues.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GRK5 Antibody (AF4539)(4)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC, Western Blot.


Filter By Application
ICC
(1)
Western Blot
(3)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
All Species

Review for GRK5 Antibody (AF4539) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF4539:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
WB Human 01/08/2015
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Summary

ApplicationWestern Blot
Sample TestedSee PMID 23139825
SpeciesHuman

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for GRK5 Antibody (AF4539) (0)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

 
Verified Customer
01/08/2015
Application: WB
Species: Human

Bioinformatics

Gene Symbol GRK5
Uniprot