Home » Gr-1/Ly-6G » Gr-1/Ly-6G Antibodies » Gr-1/Ly-6G Antibody (RB6-8C5) [Biotin]

Gr-1/Ly-6G Antibody (RB6-8C5) [Biotin]

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Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Monoclonal Antibody (Catalog # BAM1037) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Ly-6G/Ly-6C (Gr-1) was detected in immersion fixed frozen sections of mouse spleen using Rat Anti-Mouse Ly-6G/Ly-6C (Gr-1) Biotinylated Monoclonal Antibody (Catalog # BAM1037) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications Flow, IHC, IP
Clone
RB6-8C5
Clonality
Monoclonal
Host
Rat
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB1037U-100UG
Alexa Fluor 405
FAB1037V-025
Alexa Fluor 488
FAB1037G-100UG
Alexa Fluor 532
FAB1037X
Alexa Fluor 594
FAB1037T-100UG
Alexa Fluor 647
FAB1037R-100UG
Alexa Fluor 750
FAB1037S-100UG
Allophycocyanin
FAB1037A-100
Allophycocyanin
FAB1037A-025
Allophycocyanin/Cy7
FAB1037APCCY7
CoraFluor 1
FAB1037CL1
DyLight 350
FAB1037D
DyLight 405
FAB1037E
DyLight 488
FAB1037K
DyLight 550
FAB1037L
DyLight 594
FAB1037M
DyLight 650
FAB1037W
DyLight 680
FAB1037Y
DyLight 755
FAB1037Z
Fluorescein
FAB1037F-100
Fluorescein
FAB1037F-025
HRP
FAB1037H
Janelia Fluor 525
FAB1037JF525
Janelia Fluor 549
FAB1037I
Janelia Fluor 646
FAB1037J
Janelia Fluor 669
FAB1037JF669
PE/Atto594
FAB1037PEATT594
PE/Cy5.5
FAB1037PECY55
PE/Cy7
FAB1037PECY7
Phycoerythrin
FAB1037P-025
Phycoerythrin
FAB1037P-100
Unconjugated
FAB1037F-100
mFluor Violet 450 SE
FAB1037MFV450
mFluor Violet 500 SE
FAB1037MFV500
mFluor Violet 610 SE
FAB1037MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
FAB1037F-100
Carrier Free
FAB1037V-025
Carrier Free
FAB1037P-100
Carrier Free
FAB1037P-025
Carrier Free
FAB1037F-100
Carrier Free
FAB1037F-025
Carrier Free
FAB1037A-100
Carrier Free
FAB1037A-025
Carrier Free
FAB1037U-100UG
Carrier Free
FAB1037T-100UG
Carrier Free
FAB1037S-100UG
Carrier Free
FAB1037R-100UG
Carrier Free
FAB1037G-100UG
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Gr-1/Ly-6G Antibody (RB6-8C5) [Biotin] Summary

Immunogen
The immunogen for this antibody is Ly-6G (Gr-1, Gr1).
Specificity
Detects mouse Ly-6G/Ly-6C (Gr-1). Weak cross-reactivity with Ly-6C is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Rat
Gene
Ly6g
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-25 ug/mL
  • Immunoprecipitation
Readout System
Publications
Read Publication using
BAM1037 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Gr-1/Ly-6G Antibody (RB6-8C5) [Biotin]

  • Gr1
  • Gr-1
  • Ly-6C
  • Ly6G
  • Ly-6G
  • ly-6G.1
  • Ly-6G/Ly-6C (Gr-1)
  • lymphocyte antigen 6 complex, locus G
  • lymphocyte antigen 6G

Background

Gr-1, also called Ly-6G, is a myeloid differentiation antigen that is a member of the Ly-6 family. The RB6‑8C5 antibody recognizes Gr-1/Ly-6G, and also reacts weakly with Ly-6C-transfected EL4 cells (1, 2). In the periphery, this antibody specifically recognized granulocytes (3, 4).
  1. Spangrude, G.J. et al. (1988) Science 241:58
  2. Fleming, T.J. et al. (1993) J. Immunol. 151:2399
  3. Lewinsohn, D.M. et al. (1987) J. Immunol. 147:22
  4. Lagasse, E. and I. L. Weissman (1996) J. Immunol. Methods 197:139

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Gr-1/Ly-6G Antibody (BAM1037)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC-P.


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Bioinformatics

Gene Symbol Ly6g