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GPT Activity Assay Kit (Fluorometric)

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GPT Activity Assay Kit (Fluorometric) [NBP3-24460] - Standard Reference Curve
GPT Activity Assay Kit (Fluorometric) [NBP3-24460] - Assay Example

Product Details

Summary
Reactivity All-NASpecies Glossary
Applications Func
Suitable Sample Type
Serum, plasma and animal tissue
Standard Curve Range
0.01 - 0.83 U/L
Sensitivity
0.01 U/L

Order Details

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GPT Activity Assay Kit (Fluorometric) Summary

Description
Detection instrument: Fluorescence microplate reader (Ex/Em = 535 nm/587 nm)
Assay time: 75 min
Standard Curve Range
0.01 - 0.83 U/L
Sensitivity
0.01 U/L
Inter-Assay
%CV < 9.8
Intra-Assay
%CV < 2.3
Kit Type
Activity Assay Kit (Fluorometric)
Gene
GPT

Applications/Dilutions

Dilutions
  • Functional

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Black Microplate
  2. Plate Sealer
  3. Reagent 1: Buffer Solution
  4. Reagent 2: Probe Solution
  5. Reagent 3: Enzyme Reagent
  6. Reagent 4: Substrate Solution
  7. Reagent 5: 100 mmol/L Pyruvate standard

Alternate Names for GPT Activity Assay Kit (Fluorometric)

  • AAT1alanine aminotransferase 1
  • ALT1
  • ALT1EC 2.6.1.2
  • Glutamate pyruvate transaminase 1
  • glutamic-alanine transaminase 1
  • Glutamic--alanine transaminase 1
  • glutamic-pyruvate transaminase (alanine aminotransferase)
  • Glutamic--pyruvic transaminase 1
  • GPT1GPT 1

Background

GPT, also known as Alanine aminotransferase 1, is a 496 amino acid that is 55 kDa, cytoplasm located, most commonly found in liver, kidney, heart, and skeletal muscles; acts as a catalyzer of the reversible transamination between alanine and 2-oxoglutarate to compose pyruvate and glutamate, is involved in cellular nitrogen metabolism, and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Current research is being performed on several diseases and disorders including vitelliform macular dystrophy, liver disease, epidemic typhus, pyomyositis, kidney cortex necrosis, scrub typhus, hepatitis e, hepatitis c, iron overload hepatitis b, glucose intolerance, biliary atresia, alcohol abuse, cholangitis, cholecystitis, hellp syndrome, viral hepatitis, siderosis, choledocholithiasis, hepatitis a, kawasaki disease, wilson disease, and hepatic encephalopathy. This protein has shown to have interactions with CAPN1, CAPN3, HSPD1, THNSL2, GPT2, AND PSAT1 in pathways such as alanine, aspartate and glutamate metabolism, amino acid synthesis and interconversion (transamination), and metabolism of amino acids and derivatives.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Bioinformatics

Gene Symbol GPT