GPT Activity Assay Kit (Fluorometric) Summary
Description |
Detection instrument: Fluorescence microplate reader (Ex/Em = 535 nm/587 nm) Assay time: 75 min |
Standard Curve Range |
0.01 - 0.83 U/L |
Sensitivity |
0.01 U/L |
Inter-Assay |
%CV < 9.8 |
Intra-Assay |
%CV < 2.3 |
Kit Type |
Activity Assay Kit (Fluorometric) |
Gene |
GPT |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Black Microplate
- Plate Sealer
- Reagent 1: Buffer Solution
- Reagent 2: Probe Solution
- Reagent 3: Enzyme Reagent
- Reagent 4: Substrate Solution
- Reagent 5: 100 mmol/L Pyruvate standard
|
Alternate Names for GPT Activity Assay Kit (Fluorometric)
Background
GPT, also known as Alanine aminotransferase 1, is a 496 amino acid that is 55 kDa, cytoplasm located, most commonly found in liver, kidney, heart, and skeletal muscles; acts as a catalyzer of the reversible transamination between alanine and 2-oxoglutarate to compose pyruvate and glutamate, is involved in cellular nitrogen metabolism, and also in liver gluconeogenesis starting with precursors transported from skeletal muscles. Current research is being performed on several diseases and disorders including vitelliform macular dystrophy, liver disease, epidemic typhus, pyomyositis, kidney cortex necrosis, scrub typhus, hepatitis e, hepatitis c, iron overload hepatitis b, glucose intolerance, biliary atresia, alcohol abuse, cholangitis, cholecystitis, hellp syndrome, viral hepatitis, siderosis, choledocholithiasis, hepatitis a, kawasaki disease, wilson disease, and hepatic encephalopathy. This protein has shown to have interactions with CAPN1, CAPN3, HSPD1, THNSL2, GPT2, AND PSAT1 in pathways such as alanine, aspartate and glutamate metabolism, amino acid synthesis and interconversion (transamination), and metabolism of amino acids and derivatives.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are
guaranteed for 6 months from date of receipt.
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