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Recombinant Human GPR177/WLS His Protein

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SDS-Page: Recombinant Human GPR177/WLS Protein [NBP2-22947]
SDS-Page: Recombinant Human GPR177/WLS Protein [NBP2-22947]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
1 mg/ml

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Recombinant Human GPR177/WLS His Protein Summary

Description
A denatured recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 101-232 of Human GPR177/WLS

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSMEMSPWF QFMLFILQLD IAFKLNNQIR ENAEVSMDVS LAYRDDAFAE WTEMAHERVP RKLKCTFTSP KTPEHEGRYY ECDVLPFMEI GSVAHKFYLL NIRLPVNEKK KINVGIGEIK DIRLVGIHQN GGFTK
Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
WLS
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Dilutions
  • SDS-Page
Application Notes
Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.
Theoretical MW
17.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0), 0.4M UREA, 10% glycerol
Preservative
No Preservative
Concentration
1 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human GPR177/WLS His Protein

  • C1orf139
  • DKFZp686I0788
  • Evi
  • EVIchromosome 1 open reading frame 139
  • FLJ23091
  • G protein-coupled receptor 177
  • GPR177
  • GPR177wls
  • Integral membrane protein GPR177
  • MGC131760
  • MGC14878
  • MRP
  • Protein evenness interrupted homolog
  • protein wntless homolog
  • Putative NF-kappa-B-activating protein 373
  • putative NFkB activating protein 373
  • Sprinter
  • WLS
  • wntless homolog (Drosophila)
  • Wntless

Background

WLS, a Wnt trafficking regulator, is a multipass transmembrane protein that regulates the sorting and secretion of Wnt protein. It is required for organogenesis during embryogenesis and for the establishment of the anterior-posterior axis. WLS promotes various cancers such as glioma tumourigenesis. The gene encoding WLS maps to human chromosome 1 and is expressed as multiple alternatively spliced isoforms. Recombinant human WLS protein, fused to His-tag at N-terminus, was expressed in E.coli.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Recombinant Human
GPR177/WLS His Protei ...
NBP2-22947
Recombinant Human GPR177/WLS His Protein
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Bioinformatics

Gene Symbol WLS