Mouse GM-CSF ELISA Kit (Colorimetric) Summary
Description |
The mouse GM-CSF ELISA is an enzyme-linked immunosorbent assay for the quantitative detection of mouse GM-CSF. |
Specificity |
The interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a mouse GM-CSF positive serum. There was no crossreactivity detected. |
Standard Curve Range |
3.9 - 250 pg/mL |
Sensitivity |
2.0 pg/mL |
Assay Type |
Sandwich ELISA |
Inter-Assay |
CV% < 10% |
Intra-Assay |
CV% < 5.0% |
Spike Recovery |
The overall mean recovery was 85%. Please see protocol for more specific Spike Recovery details. |
Sample Volume |
50 uL |
Kit Type |
ELISA Kit (Colorimetric) |
Gene |
CSF2 |
Applications/Dilutions
Packaging, Storage & Formulations
Storage |
Storage of components varies. See protocol for specific instructions. |
Kit Components
Components
|
- Adhesive Films
- Aluminium pouch(es) with a Microwell Plate coated with monoclonal antibody to mouse GM-CSF
- Assay Buffer Concentrate 20x (PBS with 1% Tween 20 and 10% BSA)
- Biotin-Conjugate anti-mouse GM-CSF monoclonal antibody
- Mouse GM-CSF Standard lyophilized, 500 pg/ml upon reconstitution
- Sample Diluent
- Stop Solution (1M Phosphoric acid)
- Streptavidin-HRP
- Substrate Solution (tetramethyl-benzidine)
- Wash Buffer Concentrate 20x (PBS with 1% Tween 20)
|
Alternate Names for Mouse GM-CSF ELISA Kit (Colorimetric)
Background
Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a cytokine that has been characterized as a molecule capable of stimulating proliferation and differentiation of granulocyte/macrophage progenitor cells as well as modulating the functional activity of mature granulocytes and macrophages. In combination with erythropoietin, it supports the development of erythroid progenitors in vitro. The biological activities of GM-CSF substantially overlap with the effects of Interleukin 3. The reason for the redundancy of these pleiotropic cytokines may at least in part be explained by their competition for binding to common receptors. GM-CSF is produced by a number of different cell types such as activated T-cells, B-cells, macrophages, mast cells, endothelial cells, fibroblasts, synoviocytes and various tumor cells in response to various stimuli. Mouse GM-CSF as well as its receptors have been cloned. The encoded glycoprotein consists of 141 amino acid residues and has a molecular weight of about 23 kDa. The murine protein binds to a high affinity receptor complex consisting of two membrane glycoproteins, the GM-CSF receptor alpha subunit with notable homology to the human receptor and the beta subunit which also forms part of the receptors for IL-3 and IL-5. Elevated levels of GM-CSF are found in mice after endotoxin injection.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are
guaranteed for 6 months from date of receipt.
Customers Who Viewed This Item Also Viewed...
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut, WB
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA(Cap), ELISA(Det), ELISA(Sta), Neut, WB
Species: Hu
Applications: BA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Mu
Applications: ELISA
Species: Hu
Applications: BA
Species: Mu
Applications: ELISA
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu, Mu
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, WB
Species: Hu
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, IHC, IHC-P, PA, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, PEP-ELISA, WB
Species: Mu
Applications: ELISA
Publications for GM-CSF ELISA Kit (NBP1-92674) (0)
There are no publications for GM-CSF ELISA Kit (NBP1-92674).
By submitting your publication information earn gift cards and discounts for future purchases.
Reviews for GM-CSF ELISA Kit (NBP1-92674) (0)
There are no reviews for GM-CSF ELISA Kit (NBP1-92674).
By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
- Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
- Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
Product General Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
FAQs for GM-CSF ELISA Kit (NBP1-92674) (0)
Additional GM-CSF Products
Research Areas for GM-CSF ELISA Kit (NBP1-92674)
Find related products by research area.
|
Blogs on GM-CSF