Glyoxalase I Antibody (CPTC-GLO1-3) [CoraFluor™ 1]

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
CPTC-GLO1-3
Clonality
Monoclonal
Host
Mouse
Conjugate
CoraFluor 1

Order Details

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Glyoxalase I Antibody (CPTC-GLO1-3) [CoraFluor™ 1] Summary

Description
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.
Immunogen
Recombinant full-length human Glyoxalase I protein (Uniprot: Q04760)
Localization
Cytoplasm
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GLO1
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark. Do not freeze.
Buffer
PBS
Preservative
No Preservative
Purity
Protein A or G purified

Notes

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

Alternate Names for Glyoxalase I Antibody (CPTC-GLO1-3) [CoraFluor™ 1]

  • Aldoketomutase
  • EC 4.4.1.5
  • GLO1
  • GLOD1
  • Glx I
  • GLYI
  • glyoxalase domain containing 1
  • Glyoxalase I
  • glyoxalase Ialdoketomutase
  • Ketone-aldehyde mutase
  • lactoyl glutathione lyase
  • lactoylglutathione lyase
  • Methylglyoxalase
  • S-D-lactoylglutathione methylglyoxal lyase

Background

Lactoylglutathione lyase, also known as GLO1, is a member of the glyoxalase I family that is responsible for the catalysis and formation of S-lactoyl-glutathione from methylglyoxal condensation and reduced glutatione. It is believed that inactivation of GLO1 in endothelial cells contributes to vascular complications in diabetes. GLO1 has also been shown to extend lifespan in C. elegans, and has been shown to be upregulated in malignant cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GLO1