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Glutathione Peroxidase 2/GPX2 Antibody

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Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NB100-1888] - Staining (0.5ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.
Western Blot: Glutathione Peroxidase 2/GPX2 Antibody [NB100-1888] - Staining (0.5ug/ml) of Human Liver lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, CaSpecies Glossary
Applications WB, ELISA, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Glutathione Peroxidase 2/GPX2 Antibody Summary

Immunogen
Peptide with sequence C-SLDGEKVDFN corresponding to internal region according to NP_002074.2.
Predicted Species
Porcine (100%), Bovine (100%), Canine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GPX2
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
  • Immunohistochemistry-Paraffin
  • Peptide ELISA Detection limit 1:32000
  • Western Blot 0.5 - 2 ug/ml
Application Notes
WB: Approx. 24 kDa band observed in human liver lysates (calculated MW of 21.9 kDa band according to NP_002074.2). Use in IHC-P reactivity reported in (PMID: 24562575).
Control
Human Liver Whole Tissue Lysate (Adult Whole Normal)
Human Prostate Whole Tissue Lysate (Adult Whole Normal)
Glutathione Peroxidase 2/GPX2 Overexpression Lysate
Publications
Read Publications using
NB100-1888 in the following applications:

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 19151108); Rat reactivity reported in scientific literature (PMID: 18056462).

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Glutathione Peroxidase 2/GPX2 Antibody

  • EC 1.11.1
  • EC 1.11.1.9
  • gastrointestinal glutathione peroxidase 2
  • Gastrointestinal glutathione peroxidase
  • GIGPX
  • GI-GPx
  • glutathione peroxidase 2 (gastrointestinal)
  • Glutathione Peroxidase 2
  • Glutathione peroxidase-gastrointestinal
  • Glutathione peroxidase-related protein 2
  • GPRP
  • GPRP-2
  • GPX2
  • GPx-2
  • GPx-GI
  • GSHPx-2
  • GSHPX-GI

Background

This gene is a member of the glutathione peroxidase family and encodes a selenium-dependent glutathione peroxidase that is one of two isoenzymes responsible for the majority of the glutathione-dependent hydrogen peroxide-reducing activity in the epithelium of the gastrointestinal tract. Studies in knockout mice indicate that mRNA expression levels respond to luminal microflora, suggesting a role of the ileal glutathione peroxidases in preventing inflammation in the GI tract.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Glutathione Peroxidase 2/GPX2 Antibody (NB100-1888)(6)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: IF/IHC, IHC-P, WB.


Filter By Application
IF/IHC
(2)
IHC-P
(2)
WB
(3)
All Applications
Filter By Species
Human
(2)
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 6 of 6.
Publications using NB100-1888 Applications Species
Doi K, Fujioka M, Sokuza Y et al. Chemopreventive Action by Ethanol-extracted Brazilian Green Propolis on Post-initiation Phase of Inflammation-associated Rat Colon Tumorigenesis. In Vivo. 2017-03-30 [PMID: 28358699] (IF/IHC, Mouse) IF/IHC Mouse
Naiki T, Naiki-Ito A, Asamoto M et al. GPX2 overexpression is involved in cell proliferation and prognosis of castration resistant prostate cancer. Carcinogenesis. 2014-02-22 [PMID: 24562575] (IHC-P, WB, Rat, Human)

Details:
Rat castration-resistant prostate cancer model: Fig 1A [IHC(P), Fig 1B (WB); LnCAP and PC3 human prostate carcinoma cells: Fig 1B (WB); Human prostatectomy normal and cancerous specimens: Figs 4 & 5 [IHC (P)]. sections of the tissues were incubated with 1 		IHC-P, WB Rat, Human
Roland-Zejly L, Moisan V, St-Pierre I, Bilodeau JF. Altered placental glutathione peroxidase mRNA expression in preeclampsia according to the presence or absence of labor Placenta 2011-02-01 [PMID: 21145108] (IF/IHC, Human) IF/IHC Human
McGrath-Morrow S, Lauer T, Yee M et al. Nrf2 increases survival and attenuates alveolar growth inhibition in neonatal mice exposed to hyperoxia. Am J Physiol Lung Cell Mol Physiol. 2009-04-01 [PMID: 19151108] (WB, Mouse)

Details:
Newborn mouse lung tissue: WB (Fig 4B, C); IHC (paraffin): Fig 4D. 		WB Mouse
Naiki-Ito A, Asamoto M, Hokaiwado N et al. Gpx2 is an overexpressed gene in rat breast cancers induced by three different chemical carcinogens. Cancer Res. 2007-12-01 [PMID: 18056462] (WB, IHC-P, Rat)

Details:
WB: rat normal mammary and carcinogen-induced mammary cancer (Fig 2B) IHC (paraffin): rat normal mammary tissue and carcinogen-induced mammary cancer (Fig 2C) and human normal breast and breast cancer (Fig 3). 		WB, IHC-P Rat
Esworthy RS, Yang L, Frankel PH, Chu FF. Epithelium-specific glutathione peroxidase, Gpx2, is involved in the prevention of intestinal inflammation in selenium-deficient mice. J Nutr 2005-04-01 [PMID: 15795427]

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Control Lysate(s)

Human Liver Whole Tissue Lysate (Adult Whole Normal)
Human Prostate Whole Tissue Lysate (Adult Whole Normal)
Glutathione Peroxidase 2/GPX2 Overexpression Lysate

Secondary Antibodies

 

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Bioinformatics

Gene Symbol GPX2
Entrez
Uniprot