Glut1 Antibody

Glucose transporter 1 (GLUT1) or solute carrier family 2 (SLC2A1) is a member of the GLUT family of monosaccharides and polyols transporters. GLUT proteins transport glucose across cellular membranes through facilitative mechanisms and play a key role in glucose homeostasis (1). Fourteen GLUT proteins have been identified in the human, which are encoded by SLC2A genes 1-14 and are broadly expressed in many cell types and tissues. GLUT family members differ in sequence homology, substrate specificity and expression patterns. Based on sequence homology, GLUT family members are classified into Class I (GLUT1, 2, 3, 4, and GLUT14), Class II (GLUT5, 7, 9, and 11), and Class III (GLUT6, 8, 10, 12 and 13) (1). Structurally, GLUT transporters are integral membrane glycoproteins consisting of 12 membrane spanning helical domains, a single N-linked glycosylation site, and having cytoplasmic facing carboxy and amino terminal domains (2).

GLUT1 (Human glycosylated form theoretical molecular weight 55kDa) functions primarily as a glucose transporter but can transport other substrates including mannose, galactose and glucosamine across the membrane (3). Like other GLUT family members, GLUT1 is broadly expressed, nevertheless it is the predominant glucose transporter expressed in red blood cells and brain endothelial cells (1). SLC2A1 mutations underscore the autosomal dominant disorder GLUT1 deficiency syndrome (GLUTI-DS) which is characterized by low glucose levels in the brain or hypoglycorrhachia due to insufficient glucose transport across the blood brain barrier (2, 4, 5). Phenotypically, GLUT1-DS is characterized by early onset seizures, neurologic developmental delay, microcephaly, and ataxia (4). GLUT1 is highly expressed in the endothelium of cutaneous vascular lesions and serves as a marker for the diagnosis of juvenile or infantile hemangiomas (6).

References

1. Augustin, R. (2010). The protein family of glucose transport facilitators: It's not only about glucose after all. IUBMB Life. https://doi.org/10.1002/iub.315

2. Mueckler, M., & Thorens, B. (2013). The SLC2 (GLUT) family of membrane transporters. Molecular Aspects of Medicine. https://doi.org/10.1016/j.mam.2012.07.001

3. Stein, W. D., & Litman, T. (2015). Carrier-Mediated Transport. In Channels, Carriers, and Pumps. https://doi.org/10.1016/b978-0-12-416579-3.00004-6

4. Pearson, T. S., Akman, C., Hinton, V. J., Engelstad, K., & De Vivo, D. C. (2013). Phenotypic spectrum of glucose transporter type 1 deficiency syndrome (Glut1 DS). Current Neurology and Neuroscience Reports. https://doi.org/10.1007/s11910-013-0342-7

5. Messana, T., Russo, A., Vergaro, R., Boni, A., Santucci, M., & Pini, A. (2018). Glucose transporter type 1 deficiency syndrome: Developmental delay and early-onset ataxia in a novel mutation of the SLC2A1 gene. Journal of Pediatric Neurosciences. https://doi.org/10.4103/JPN.JPN_169_17

6. van Vugt, L. J., van der Vleuten, C. J. M., Flucke, U., & Blokx, W. A. M. (2017). The utility of GLUT1 as a diagnostic marker in cutaneous vascular anomalies: A review of literature and recommendations for daily practice. Pathology Research and Practice. https://doi.org/10.1016/j.prp.2017.04.023

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Fish, Zebrafish.

We have publications tested in 5 applications: ICC/IF, IF/IHC, IHC, IHC-P, WB.

Showing Publications 1 - 10 of 29. Show All 29 Publications. Collapse Publications.

Publications using NB300-666	Applications	Species
Finicle BT Targeting endolysosomal trafficking with synthetic sphingolipid analogs to improve the delivery of oligonucleotide therapeutics Thesis 2023-01-01 (ICC/IF)	ICC/IF
Parab S, Card OA, Chen Q et al. Local angiogenic interplay of Vegfc/d and Vegfa controls brain region-specific emergence of fenestrated capillaries eLife 2023-05-16 [PMID: 37191285] (IHC-P, Fish) Details: 1:200 IHC-P dilution	IHC-P	Fish
Fetsko AR, Sebo DJ, Taylor MR Brain endothelial cells acquire blood-brain barrier properties in the absence of Vegf-dependent CNS angiogenesis Developmental biology 2022-12-09 [PMID: 36502932] (IHC, Zebrafish)	IHC	Zebrafish
Langbein LE, El Hajjar R, He S et al. BAP1 maintains HIF-dependent interferon beta induction to suppress tumor growth in clear cell renal cell carcinoma Cancer letters 2022-08-20 [PMID: 35995140] (WB, Human) Details: Dilutions: 1:2000	WB	Human
Harder I, MUnchhalfen M, Andrieux G et al. Dysregulated PI3K Signaling in B Cells of CVID Patients Cells 2022-01-28 [PMID: 35159274] (Human)		Human
Kanan Y, Hackett SF, Taneja K et al. Oxidative stress-induced alterations in retinal glucose metabolism in Retinitis Pigmentosa Free radical biology & medicine 2022-03-01 [PMID: 35134532] (WB, Mouse)	WB	Mouse
Laitakari A, Huttunen R, Kuvaja P et al. Systemic long-term inactivation of hypoxia-inducible factor prolyl 4-hydroxylase 2 ameliorates aging-induced changes in mice without affecting their life span FASEB J. 2020-02-25 [PMID: 32100354] (WB, Mouse)	WB	Mouse
Chowdhury A, Aich A, Jain G et al. Defective Mitochondrial Cardiolipin Remodeling Dampens HIF-1a Expression in Hypoxia. Cell Rep. 2018-10-16 [PMID: 30332638] (WB, Mouse)	WB	Mouse
Miyazawa H, Yamamoto M, Yamaguchi Y et al. Mammalian embryos show metabolic plasticity toward the surrounding environment during neural tube closure Genes Cells 2018-08-08 [PMID: 30088697] (Rat)		Rat
Finicle BT, Ramirez MU, Liu G et al. Sphingolipids inhibit endosomal recycling of nutrient transporters by inactivating ARF6. J Cell Sci 2018-06-25 [PMID: 29848659]
Kim SM, Roy SG, Chen B et al. Targeting cancer metabolism by simultaneously disrupting parallel nutrient access pathways J. Clin. Invest. 2016-09-26 [PMID: 27669461] (ICC/IF, Mouse)	ICC/IF	Mouse
Joyal JS, Sun Y, Gantner ML et al. Retinal lipid and glucose metabolism dictates angiogenesis through the lipid sensor Ffar1. Nat Med 2016-04-01 [PMID: 26974308]
Ulrich F, Carretero-Ortega J, MenEndez J et al. Reck enables cerebrovascular development by promoting canonical Wnt signaling Development. 2015-12-31 [PMID: 26657775] (IF/IHC, Zebrafish)	IF/IHC	Zebrafish
Lin AP, Abbas S, Kim SW et al. D2HGDH regulates alpha-ketoglutarate levels and dioxygenase function by modulating IDH2 Nat Commun. 2015-07-16 [PMID: 26178471] (WB, Human)	WB	Human
Vanhollebeke B, Stone OA, Bostaille N et al. Tip cell-specific requirement for an atypical Gpr124- and Reck-dependent Wnt/beta-catenin pathway during brain angiogenesis. Elife 2015-01-01 [PMID: 26051822]
Zhang T, Niu X, Liao L et al. The Contributions of HIF-Target Genes to Tumor Growth in RCC. PLoS One. 2013-11-18 [PMID: 24260413] (WB, Human)	WB	Human
Couch JA, Yu YJ, Zhang Y et al. Addressing Safety Liabilities of TfR Bispecific Antibodies That Cross the Blood-Brain Barrier. Sci Transl Med 2013-05-01 [PMID: 23636093]
Wang Y, Thompson JD, Chan WK. A cell-penetrating peptide suppresses the hypoxia inducible factor-1 function by binding to the helix-loop-helix domain of the aryl hydrocarbon receptor nuclear translocator. Chem Biol Interact 2013-02-27 [PMID: 23454269] (WB, Human)	WB	Human
Koivunen P, Lee S, Duncan CG et al. Transformation by the (R)-enantiomer of 2-hydroxyglutarate linked to EGLN activation. Nature 2012-03-22 [PMID: 22343896] (Human)		Human
Kuzmanov A, Wielockx B, Rezaei M et al. Overexpression of factor inhibiting HIF-1 enhances vessel maturation and tumor growth via platelet-derived growth factor-C. International Journal of Cancer 2011-11-18 [PMID: 22095574]
Zhou L, Yang H. The von Hippel-Lindau tumor suppressor protein promotes c-Cbl-independent poly-ubiquitylation and degradation of the activated EGFR. PLoS One 2011-01-01 [PMID: 21949687]
Pena-Llopis S, Vega-Rubin-de-Celis S, Schwartz JC, et al. Regulation of TFEB and V-ATPases by mTORC1 EMBO J 2011-07-01 [PMID: 21804531]
Niu X, Zhang T, Liao L et al. The von Hippel-Lindau tumor suppressor protein regulates gene expression and tumor growth through histone demethylase JARID1C. Oncogene 2012-02-09 [PMID: 21725364] (WB)	WB
Yu Y, Maguire TG, Alwine JC. Human Cytomegalovirus Activates Glucose Transporter 4 Expression to Increase Glucose Uptake during Infection. J Virol. 2010-12-08 [PMID: 21147915]
Hyvarinen J, Hassinen IE, Sormunen R et al. Hearts of hypoxia-inducible factor prolyl 4-hydroxylase-2 hypomorphic mice show protection against acute ischemia-reperfusion injury. J Biol Chem. 2010-04-30 [PMID: 20185832] (WB, Mouse)	WB	Mouse
Luo T, Zhang X, Nicholson WL et al. Molecular characterization of antibody epitopes of Ehrlichia chaffeensis ankyrin protein 200 and tandem repeat protein 47 and evaluation of synthetic immunodeterminants for serodiagnosis of human monocytotropic ehrlichiosis. Clin Vaccine Immunol 2010-01-01 [PMID: 19955322]
Minamishima YA, Moslehi J, Padera RF et al. A feedback loop involving the Phd3 prolyl hydroxylase tunes the mammalian hypoxic response in vivo. Mol Cell Biol 2009-11-01 [PMID: 19720742]
Minamishima YA, Moslehi J, Bardeesy N et al. Somatic inactivation of the PHD2 prolyl hydroxylase causes polycythemia and congestive heart failure. Blood;111(6):3236-44. 2008-03-15 [PMID: 18096761] (WB, Mouse)	WB	Mouse
Kariuki TM, Van Dam GJ, Deelder AM et al. Previous or ongoing schistosome infections do not compromise the efficacy of the attenuated cercaria vaccine. Infect Immun 2006-07-01 [PMID: 16790771]
Show All 29 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	3 reviewed by: Verified Customer	WB	Human	10/04/2015	View Summary Application Western Blot Sample Tested Human cancer cell whole cell lysate Species Human
	4 reviewed by: Verified Customer	WB	Human	12/23/2014	View Summary Application Western Blot Sample Tested See PMID 24260413 Species Human

Array NB300-666

• Glut1 Antibodies
• Glut1 ELISA Kits
• Glut1 Lysate
• Glut1 Proteins