![Western Blot: GLP/EHMT1 Antibody [NBP3-18679] - Figure 2. Western blot analysis using the Diagenode antibody directed against EHMT1Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against EHMT1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.](http://images.novusbio.com/fullsize/GLP-EHMT1-Antibody-Western-Blot-NBP3-18679-img0001.jpg "Western Blot: GLP/EHMT1 Antibody [NBP3-18679] - Figure 2. Western blot analysis using the Diagenode antibody directed against EHMT1Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using the antibody against EHMT1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest (expected size: 120 kDa) is indicated on the right; the marker (in kDa) is shown on the left. The smaller fragment of approximately 92 kDa may represent a splicing variant.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Immunogen | Polyclonal antibody raised in rabbit against mouse GLP/EHMT1 (euchromatic histone-lysine N-methyltransferase 1), using three KLH-conjugated synthetic peptides containing a sequence from the central part of the protein (1). |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | EHMT1 |
| Purity | Whole Antiserum |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
|
| Storage | Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles. |
| Buffer | Whole antiserum |
| Preservative | 0.05% Sodium Azide |
| Purity | Whole Antiserum |
Secondary Antibodies |
Isotype Controls |
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![ELISA: GLP/EHMT1 Antibody [NBP3-18679] - Figure 1. Determination of the titerTo determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human EHMT1. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.](http://images.novusbio.com/fullsize/GLP-EHMT1 Antibody-ELISA-NBP3-18679-img0002.jpg "ELISA: GLP/EHMT1 Antibody [NBP3-18679] - Figure 1. Determination of the titerTo determine the titer, an ELISA was performed using a serial dilution of the antibody directed against human EHMT1. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:2,000.")
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