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GKAP/DLGAP1 Antibody (727425)

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GKAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human GKAP Monoclonal Antibody (Catalog # MAB7296) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
GKAP was detected in immersion fixed paraffin-embedded sections of human brain (cortex) using Mouse Anti-Human GKAP Monoclonal Antibody (Catalog # MAB7296) at 15 µg/mL overnight at 4 °C. Before incubation with the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC
Clone
727425
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB7296-SP
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GKAP/DLGAP1 Antibody (727425) Summary

Immunogen
E. coli-derived recombinant human GKAP
Lys368-Glu472
Accession # O14490
Specificity
Detects human GKAP in direct ELISAs.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
DLGAP1
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GKAP/DLGAP1 Antibody (727425)

  • DAP1
  • DAP-1-ALPHA
  • DAP-1DAP-1-BETA
  • discs, large (Drosophila) homolog-associated protein 1
  • disks large-associated protein 1
  • DLGAP1
  • GKAP
  • GKAPMGC88156
  • hGKAP
  • PSD-95/SAP90 binding protein 1
  • PSD-95/SAP90-binding protein 1
  • SAP90/PSD-95-associated protein 1
  • SAPAP1
  • SAPAP1Guanylate kinase-associated protein

Background

GKAP, also known as DAP1, DLGAP, and SAPAP1, is an approximately 45 kDa cytoplasmic membrane associated protein. GKAP is a component of a neuronal postsynaptic complex that also includes PSD-95 and SHANK. This complex links neurotransmitter channels with the cytoskeleton and can promote NMDA R channel opening. Alternate splice forms of human GKAP have N-terminal and/or C-terminal truncations and are differentially expressed during brain development. Within aa 368-472 human, mouse, and rat GKAP share 100% aa sequence identity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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GKAP/DLGAP1
Antibody (727425)
MAB7296
GKAP/DLGAP1 Antibody (727425)
Species: Hu
Applications: IHC

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Bioinformatics

Gene Symbol DLGAP1
Uniprot