GITR Ligand/TNFSF18 Antibody (109114) [Alexa Fluor® 647] Summary
Immunogen |
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human GITR Ligand/TNFSF18 Glu52-Ser177 Accession # Q9UNG2 |
Specificity |
Detects human GITR Ligand/TNFSF18 in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant human (rh) APRIL, rhLIGHT, rhTNF-alpha , or rhVEGI is observed.
|
Isotype |
IgG1 |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
TNFSF18 |
Purity Statement |
Protein A or G purified from hybridoma culture supernatant |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Flow Cytometry 0.25-1 ug/10^6 cells
|
Application Notes |
Flow Cytometry: Please use 0.25-1 ug of conjugated antibody per 10e6 cells. |
Packaging, Storage & Formulations
Storage |
Store the unopened product at 2 - 8 °C. Do not use past expiration date. |
Buffer |
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide. |
Preservative |
0.09% Sodium Azide |
Concentration |
Please see the vial label for concentration. If unlisted please contact technical services. |
Notes
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for GITR Ligand/TNFSF18 Antibody (109114) [Alexa Fluor® 647]
Background
GITR (glucocorticoid-induced TNF receptor superfamily-related protein, also named AITR, activation-inducible TNF receptor superfamily-related protein) and GITR Ligand (GITRL) are novel members of the TNF receptor (TNFR) and TNF superfamilies (SF) that have been designated TNFRSF18 and TNFSF18, respectively. Human GITR Ligand cDNA encodes a 177 amino acid type II membrane protein. The carboxy-terminal extracellular domain shows sequence identity to TNF/TNFSF2 (21%), Fas ligand/TNFSF6 (21%), TRAIL/TNFSF10 (18%), and lymphotoxin alpha /TNFSF1 (18%). GITR Ligand is constitutively expressed in human umbilical vein endothelial cells but is not expressed in resting or stimulated T cell lines, B cell lines or peripheral blood mononuclear cells. GITR, the receptor for GITR Ligand, is expressed at low levels in peripheral blood T cells, bone marrow, thymus, spleen and lymph nodes. In contrast to mouse GITR, expression of human GITR is not induced by treatment with dexamethasone, but is up-regulated by antigen-receptor stimulation or by treatment with soluble anti-CD3 plus anti-CD28 or PMA plus ionomycin. Ligation of GITR has been found to induce nuclear factor (NF)-kappa B activation via TNF receptor-associated factor 2 and protect cells from TCR activation-induced cell death. It has been proposed that GITR Ligand and GITR may modulate T lymphocyte functions in peripheral tissues.
- Nocentini, G. et al. (1997) Proc. Natl. Acad. Sci. USA 94:6216.
- Kwon, B. et al. (1999) J. Biol. Chem. 274:6056.
- Gurney, A.L. et al. (1999) Curr. Biol. 9:215.
- Kwon, B. et al. (1999) Curr. Opin. Immunol. 11:340.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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