Home » GFR alpha-2/GDNF R alpha-2 » GFR alpha-2/GDNF R alpha-2 Lysates » GFR alpha-2/GDNF R alpha-2 Overexpression Lysate

GFR alpha-2/GDNF R alpha-2 Overexpression Lysate

 Submit a Review

Images

 
(
Enlarge)
Western Blot: GDNF Receptor alpha 2 Overexpression Lysate (Adult Normal) [NBL1-11052] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GDNF Receptor alpha 2.
Western Blot: GDNF Receptor alpha 2 Overexpression Lysate (Adult Normal) [NBL1-11052] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for GDNF Receptor alpha 2.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Novus Biologicals offers 100% Guarantee

GFR alpha-2/GDNF R alpha-2 Overexpression Lysate Summary

Description

GFR alpha-2/GDNF R alpha-2 Transient Overexpression Lysate


Expression Host: HEK293T

Plasmid: RC208014

Accession#: NM_001495

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
Gene
GFRA2

Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.

Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW
49 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for GFR alpha-2/GDNF R alpha-2 Overexpression Lysate

  • GDNF family receptor alpha 2
  • GDNF family receptor alpha-2
  • GDNF R alpha-2
  • GDNF receptor alpha-2
  • GDNF receptor beta
  • GDNFR-alpha-2
  • GDNFR-beta
  • GDNFRBRET ligand 2
  • GFR alpha2
  • GFR alpha-2
  • GFRA2
  • GFRa-2
  • GFR-alpha 2
  • GFR-alpha-2
  • glial cell line derived neurotrophic factor receptor, beta
  • Neurturin receptor alpha
  • NRTNR-alpha
  • NTNRA
  • NTNR-alpha
  • PI-linked cell-surface accessory protein
  • RETL2TGF-beta-related neurotrophic factor receptor 2
  • TGF-beta related neurotrophic factor receptor 2
  • TRN receptor, GPI-anchored
  • TRNR2NRTNR-ALPHA

Background

Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Neurturin
1297-NE/CF
Neurturin
(9 Publications)
Species: Hu
Applications: Bind, BA
GDNF [Unconjugated]
212-GD
Bioactivity GDNF [Unconjugated]
SDS-PAGE GDNF [Unconjugated]
SEC-MALS GDNF [Unconjugated]
(223 Publications)
Species: Hu
Applications: Bind, BA
Ret Antibody
[Unconjugated]
AF482
Immunohistochemistry Ret Antibody [Unconjugated]
Western Blot Ret Antibody [Unconjugated]
Immunohistochemistry Ret Antibody [Unconjugated]
(40 Publications)
Species: Mu
Applications: IHC, WB
GFR alpha-1/GDNF R
alpha-1 Antibody [Unc ...
AF560
Immunohistochemistry GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]
Western Blot GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]
Immunocytochemistry/ Immunofluorescence GFR alpha-1/GDNF R alpha-1 Antibody [Unconjugated]
(8 Reviews)
(72 Publications)
Species: Rt
Applications: Block, IHC, WB
CX3CL1/Fractalkine
Antibody [Unconjugate ...
AF365
Neutralization CX3CL1/Fractalkine Antibody [Unconjugated]
Western Blot CX3CL1/Fractalkine Antibody [Unconjugated]
(2 Reviews)
(33 Publications)
Species: Hu
Applications: Neut, WB
Artemin
1085-AR
Artemin
(10 Publications)
Species: Mu
Applications: Bind, BA
GFR alpha-3/GDNF R
alpha-3 Antibody [Unc ...
AF2645
Immunohistochemistry GFR alpha-3/GDNF R alpha-3 Antibody [Unconjugated]
(17 Publications)
Species: Mu
Applications: IHC, WB
Recombinant Human
Persephin Protein
NBP2-35208
SDS-PAGE Recombinant Human Persephin Protein
Species: Hu
Applications: BA, PAGE
NT-3 [Unconjugated]
267-N3
NT-3 [Unconjugated]
(73 Publications)
Species: Hu
Applications: BA
BDNF [HRP]
DBD00
N/A BDNF [HRP]
N/A BDNF [HRP]
N/A BDNF [HRP]
(104 Publications)
Species: Hu
Applications: ELISA
RAPGEF5 Antibody -
BSA Free
NBP2-57362
Western Blot RAPGEF5 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence RAPGEF5 Antibody - BSA Free
Species: Hu
Applications: ICC/IF, WB
TrkA Antibody
[Unconjugated]
AF1056
Immunohistochemistry TrkA Antibody [Unconjugated]
Western Blot TrkA Antibody [Unconjugated]
Immunohistochemistry TrkA Antibody [Unconjugated]
(48 Publications)
Species: Rt
Applications: IHC, WB
EGR1 Antibody
[Unconjugated]
AF2818
Immunocytochemistry EGR1 Antibody [Unconjugated]
(13 Publications)
Species: Hu
Applications: ICC, WB
Tyrosine
Hydroxylase Antibody ...
NB300-109
Immunohistochemistry-Frozen Tyrosine Hydroxylase Antibody - Azide Free
Immunohistochemistry Tyrosine Hydroxylase Antibody - Azide Free
Immunohistochemistry Tyrosine Hydroxylase Antibody - Azide Free
(7 Reviews)
(240 Publications)
Species: Ch, Dr, Hu, I, Ma, Mu, Rt
Applications: Dual ISH-IHC, ICC/IF, IHC-FrFl, IHC-WhMt, IHC, IHC-Fr, IHC-P, KO, Simple Western, WB
ERK1 Antibody
(1E5) - BSA Free
NBP2-22203
Flow Cytometry ERK1 Antibody (1E5) - BSA Free
Immunocytochemistry/ Immunofluorescence ERK1 Antibody (1E5) - BSA Free
Immunohistochemistry-Paraffin ERK1 Antibody (1E5) - BSA Free
(1 Publication)
Species: Hu, Pm, Mu, Rt
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
TrkB Antibody
[Unconjugated]
AF1494
Immunohistochemistry TrkB Antibody [Unconjugated]
Western Blot TrkB Antibody [Unconjugated]
Western Blot TrkB Antibody [Unconjugated]
(12 Reviews)
(82 Publications)
Species: Hu, Mu, Rt
Applications: Block, IHC, Simple Western, WB

Publications for GFR alpha-2/GDNF R alpha-2 Lysate (NBL1-11052) (0)

There are no publications for GFR alpha-2/GDNF R alpha-2 Lysate (NBL1-11052).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GFR alpha-2/GDNF R alpha-2 Lysate (NBL1-11052) (0)

There are no reviews for GFR alpha-2/GDNF R alpha-2 Lysate (NBL1-11052). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GFR alpha-2/GDNF R alpha-2 Lysate (NBL1-11052) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional GFR alpha-2/GDNF R alpha-2 Products

Research Areas for GFR alpha-2/GDNF R alpha-2 Lysate (NBL1-11052)

Find related products by research area.

Blogs on GFR alpha-2/GDNF R alpha-2

There are no specific blogs for GFR alpha-2/GDNF R alpha-2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

  SDS
  PDF Datasheet
  Request CofA

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GFR alpha-2/GDNF R alpha-2 Overexpression Lysate and receive a gift card or discount.


Bioinformatics

Gene Symbol GFRA2