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GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

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Western Blot: GFR alpha-1/GDNF R alpha-1 Antibody [NBP1-77043] - Analysis of GFR alpha 1 in crude membrane fractions of human brain (B), liver (L), kidney (K), and spleen (S), respectively, with GFR alpha 1 antibody at ...read more
Immunocytochemistry/ Immunofluorescence: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] - Immunofluorescence of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 20 u/mL.Red: GFR alpha 1 ...read more
Immunohistochemistry: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] - Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 1 ug/mL.
Immunohistochemistry: GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free [NBP1-77043] - Immunohistochemistry of GFR alpha 1 in human brain tissue with GFR alpha 1 antibody at 2.5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ELISA, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

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GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free Summary

Immunogen
Antibody was raised against a 14 amino acid peptide near the center of human GFR alpha 1. The immunogen is located within amino acids 360 - 410 of GFR alpha 1. Amino Acid Squence: KNKPLGPAGSENEI
Specificity
GFR alpha 1 antibody is predicted to not cross-react with other members of the GFR alpha family of proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GFRA1
Purity
Peptide affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 20 ug/ml
  • Immunohistochemistry 1 ug/ml
  • Immunohistochemistry-Paraffin 1 ug/ml
  • Western Blot 1 ug/ml
Theoretical MW
51 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
GFR alpha-1/GDNF R alpha-1 Peptide (NBP1-77043PEP)
Publications
Read Publications using NBP1-77043.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Peptide affinity purified

Alternate Names for GFR alpha-1/GDNF R alpha-1 Antibody - BSA Free

  • DKFZp313E1012
  • DKFZp686J0156
  • GDNF family receptor alpha 1
  • GDNF family receptor alpha-1
  • GDNF R alpha-1
  • GDNF receptor alpha-1
  • GDNFRAGFR-ALPHA-1
  • GDNFR-alpha-1
  • GDNFRMGC23045
  • GFR alpha1
  • GFR alpha-1
  • GFRA1
  • GFRa-1
  • GFR-alpha-1
  • Glial cell line-derived neurotrophic factor receptor alpha
  • GPI-linked anchor protein
  • PI-linked cell-surface accessory protein
  • RET ligand 1
  • RET1L
  • RETL1FLJ10561
  • TGF-beta related neurotrophic factor receptor 1
  • TGF-beta-related neurotrophic factor receptor 1
  • TRNR1FLJ31546

Background

Glial cell line-derived neurotrophic factor (GDNF) is a potent survival factor for central and peripheral neurons and is essential for the development of kidneys and the enteric nerves system. Physiological responses to GDNF require the presence of a novel glycosylphosphadidylinositol linked protein GDNFRalpha, which is a cell surface receptor for GDNF. The cDNAs encoding GDNFRalpha from human, rat, chicken and mouse have been cloned recently. GDNFRalpha was also termed Ret ligand 1 (RETL1) or TGF-beta-related neurotrophic factor receptor 1 (TrnR1) and nominated as GFRalpha-1 recently. GFRalpha-1 binds GDNF specifically and mediates activation of the Ret protein tyrosine kinase (PTK). Thus, GDNF, GFRalpha and the Ret PTK form a complex to transduce GDNF signal and to mediate GDNF function.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GFR alpha-1/GDNF R alpha-1 Antibody (NBP1-77043)(3)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GFR alpha-1/GDNF R alpha-1 Antibody (NBP1-77043). (Showing 1 - 1 of 1 FAQs).

  1. Could you please let me know if the following antibodies recognize an internal epitope : - NBP1-77043 (eptiope is between aa 369-382 : are they internal aa ?) - NB100-91781 (epitope is arround lysine 90 : is it an internal epitope ?)
    • GFRA1 is 405 amino acidsd when full processed http://www.uniprot.org/uniprot/P56159). This processed form corresponds to amino acids 25-429, so these epitopdes correspond to being in the internal portion of GFRA1. This receptor is GPI-linked, so there is no transmembrane domain and therefore no cytoplasmic domain of this protein.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GFRA1
Uniprot