Novus Biologicals products are now on bio-techne.com

GEMIN2 Antibody (OTI4C11) - Azide and BSA Free

Images

 
Western Blot: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GEMIN2 .
Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374] - Analysis of Human lymphoma tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374] - Analysis of Human lymph node tissue. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)
Immunohistochemistry: GEMIN2 Antibody (OTI4C11) - Azide and BSA Free [NBP2-71374] - Analysis of Human tonsil tissue.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120C for 3 min)(1:150)

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI4C11
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
LYOPH

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

GEMIN2 Antibody (OTI4C11) - Azide and BSA Free Summary

Immunogen
Full length human recombinant protein of human GEMIN2(NP_003607) produced in E.coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
GEMIN2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot 1:2000
Theoretical MW
31.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Lyophilized from PBS (pH 7.3) with 8% Trehalose
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Alternate Names for GEMIN2 Antibody (OTI4C11) - Azide and BSA Free

  • Component of gems 2
  • Gemin-2
  • GEMIN2survival of motor neuron protein-interacting protein 1
  • SIP1-delta
  • SMN interacting protein 1-delta
  • SMN-interacting protein 1
  • survival of motor neuron protein interacting protein 1

Background

SIP1 encodes one of the proteins found in the SMN complex, which consists of the suvival of motor neuron protein and several gemin proteins. The SMN complex is localized to a subnuclear compartment called gems (gemini of coiled bodies) and is required for assembly of spliceosomal snRNPs and for pre-mRNA splicing. This protein interacts directly with the survival of motor neuron protein and it is required for formation of the SMN complex. A knockout mouse targeting the mouse homolog of this gene exhibited disrupted snRNP assembly and motor neuron degeneration.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-82991
Species: Hu, Mu
Applications: Flow, ICC/IF, IHC, IHC-P, KD, WB
H00006607-M01
Species: Hu
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
NB100-1936
Species: Hu, Mu, Pm, Rt, Xp, Ze
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, IP, WB
NBP2-81851
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, WB
AF748
Species: Hu, Mu
Applications: CyTOF-ready, Dual ISH-IHC, Flow, ICC, IHC, Simple Western, WB
NBP1-76798
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IHC, IHC-P, KO, WB
NBP1-05987
Species: Hu, Mu, Rt
Applications: ChIP, GS, ICC/IF, IHC, IHC-P, IP, KD, MiAr, Simple Western, Single-Cell Western, WB
NBP1-83976
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP3-25538
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-52454
Species: Hu, Rt
Applications: CyTOF-ready, ELISA, Flow, ICC/IF, WB
NBP2-03886
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
H00079833-B01P
Species: Hu
Applications: ICC/IF, WB
NBP2-37364
Species: Hu, Mu
Applications: CyTOF-ready, ELISA, Flow-IC, Flow, ICC/IF, IHC, IHC-P, KD, WB
NB300-223
Species: Bv, Ca, Ch, Eq, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, WB
NB110-40591
Species: Hu
Applications: ICC/IF, IHC, IHC-P, IP, WB
AF3639
Species: Hu
Applications: ChIP, ICC, ICFlow, WB
NBP1-48309
Species: Hu, Mu, Rt
Applications: Flow-IC, Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NB100-61049
Species: Ba, SyHa, Hu, Po
Applications: IP, WB
NLS54
Species: Ca, Hu, Mu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-33923
Species: Hu
Applications: ICC/IF, WB

Publications for GEMIN2 Antibody (NBP2-71374) (0)

There are no publications for GEMIN2 Antibody (NBP2-71374).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for GEMIN2 Antibody (NBP2-71374) (0)

There are no reviews for GEMIN2 Antibody (NBP2-71374). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for GEMIN2 Antibody (NBP2-71374) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional GEMIN2 Products

Research Areas for GEMIN2 Antibody (NBP2-71374)

Find related products by research area.

Blogs on GEMIN2

There are no specific blogs for GEMIN2, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our GEMIN2 Antibody (OTI4C11) - Azide and BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol GEMIN2