GDNF Antibody

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. NS0 expressed mature human GDNF lacks 31 residues from the amino-terminus of the predicted sequence. This glycosylated recombinant mature human GDNF still contains the seven conserved Cys residues found in all members of the TGF-beta superfamily and is biologically active. The GDNF sequence contains two potential glycosylation sites and insect cell-expressed recombinant rat GDNF proteins are glycosylated. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Chicken, N/A, Primate - Callitrix jacchus (Common Marmoset), Primate - Macaca mulatta (Rhesus Macaque), Primate - Rhesus macaque.

We have publications tested in 9 applications: Affinity Assay, ELISA Development (Detection), Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Neutralization, Western Blot.

Showing Publications 1 - 10 of 46. Show All 46 Publications. Collapse Publications.

Publications using AF-212-NA	Applications	Species
Ford, MM;George, BE;Van Laar, VS;Holleran, KM;Naidoo, J;Hadaczek, P;Vanderhooft, LE;Peck, EG;Dawes, MH;Ohno, K;Bringas, J;McBride, JL;Samaranch, L;Forsayeth, JR;Jones, SR;Grant, KA;Bankiewicz, KS; GDNF gene therapy for alcohol use disorder in male non-human primates Nature medicine 2023-08-01 [PMID: 37580533] (IHC, Primate - Rhesus macaque)	IHC	Primate - Rhesus macaque
Yuki Saito, Maki Miyajima, Sena Yamamoto, Norihiro Miura, Tsukasa Sato, Arisa Kita et al. Intrathecal Injection of Mesenchymal Stromal Cell Cultured on 3D Fiber Ameliorates Multiple Organ Damage in Murine Lupus Stem Cells Translational Medicine 6/22/2022 [PMID: 35466994]
N Sultan, BA Scheven Exploring the potential application of dental pulp stem cells in neuroregenerative medicine Neural regeneration research, 2022-04-01;17(4):775-776. 2022-04-01 [PMID: 34472466] (Neutralization, Rat)	Neutralization	Rat
N Sultan, LE Amin, AR Zaher, ME Grawish, BA Scheven Dental pulp stem cells stimulate neuronal differentiation of PC12 cells Neural regeneration research, 2021-09-01;16(9):1821-1828. 2021-09-01 [PMID: 33510089] (Neutralization, Rat)	Neutralization	Rat
J Yin, HM Chang, Y Yi, Y Yao, PCK Leung TGF-beta1 Increases GDNF Production by Upregulating the Expression of GDNF and Furin in Human Granulosa-Lutein Cells Cells, 2020-01-10;9(1):. 2020-01-10 [PMID: 31936902] (Western Blot, Human)	Western Blot	Human
James W. H. Sonne, Jason S. Groshong, Corey Seavey, Don M. Gash Spatial and temporal immunoreactivity in the rat brain using an affinity purified polyclonal antibody to DNSP-11 Journal of Chemical Neuroanatomy 10/1/2019 [PMID: 31394198]
Dwaine F. Emerich, Jeffrey H. Kordower, Yaping Chu, Chris Thanos, Briannan Bintz, Giovanna Paolone et al. Widespread Striatal Delivery of GDNF from Encapsulated Cells Prevents the Anatomical and Functional Consequences of Excitotoxicity Neural Plasticity 3/11/2019 [PMID: 30984255]
Masao Nakasatomi, Shunsuke Takahashi, Toru Sakairi, Hidekazu Ikeuchi, Yoriaki Kaneko, Keiju Hiromura et al. Enhancement of HGF-induced tubulogenesis by endothelial cell-derived GDNF PLOS ONE 3/7/2019 [PMID: 30845150]
L Quintino, A Namislo, M Davidsson, LS Breger, P Kavanagh, M Avallone, E Elgstrand-, C Isaksson, C Lundberg Destabilizing Domains Enable Long-Term and Inert Regulation of GDNF Expression in the Brain Mol Ther Methods Clin Dev, 2018-09-04;11(0):29-39. 2018-09-04 [PMID: 30324128] (IHC, Rat)	IHC	Rat
Anna-Maija Penttinen, Ilmari Parkkinen, Merja H. Voutilainen, Maryna Koskela, Susanne Bäck, Anna Their et al. Pre-alpha -pro-GDNF and Pre-beta -pro-GDNF Isoforms Are Neuroprotective in the 6-hydroxydopamine Rat Model of Parkinson's Disease Frontiers in Neurology 6/20/2018 [PMID: 29973907]
R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies Proc. Natl. Acad. Sci. U.S.A., 2018-01-16;0(0):. 2018-01-16 [PMID: 29339495] (Affinity Assay)	Affinity Assay
Richard Mills, Hermes Taylor-Weiner, Jorge C. Correia, Leandro Z. Agudelo, Ilary Allodi, Christina Kolonelou et al. Neurturin is a PGC-1 alpha 1-controlled myokine that promotes motor neuron recruitment and neuromuscular junction formation Molecular Metabolism 1/1/2018 [PMID: 29157948]
K Nagashima, T Miwa, H Soumiya, D Ushiro, T Takeda-Kaw, N Tamaoki, S Ishiguro, Y Sato, K Miyamoto, T Ohno, M Osawa, T Kunisada, T Shibata, KI Tezuka, S Furukawa, H Fukumitsu Priming with FGF2 stimulates human dental pulp cells to promote axonal regeneration and locomotor function recovery after spinal cord injury Sci Rep, 2017-10-18;7(1):13500. 2017-10-18 [PMID: 29044129] (ICC, Human)	ICC	Human
J Lavaur, D Le Nogue, M Lemaire, J Pype, G Farjot, EC Hirsch, PP Michel The Noble Gas Xenon Provides Protection and Trophic Stimulation to Midbrain Dopamine Neurons J. Neurochem., 2017-05-16;0(0):. 2017-05-16 [PMID: 28398653] (Neutralization, Rat)	Neutralization	Rat
Y Dong, A Kanda, K Noda, W Saito, S Ishida Pathologic Roles of Receptor-Associated Prorenin System in Idiopathic Epiretinal Membrane Sci Rep, 2017-03-09;7(0):44266. 2017-03-09 [PMID: 28276504] (IHC-P, Human)	IHC-P	Human
GM Thomsen, M Alkaslasi, JP Vit, G Lawless, M Godoy, G Gowing, O Shelest, CN Svendsen Systemic injection of AAV9-GDNF provides modest functional improvements in the SOD1(G93A) ALS rat but has adverse side effects Gene Ther, 2017-03-09;0(0):. 2017-03-09 [PMID: 28276446] (IHC, Rat)	IHC	Rat
SR Stott, S Hayat, T Carnwath, S Garas, JP Sleeman, RA Barker CD24 expression does not affect dopamine neuronal survival in a mouse model of Parkinson's disease PLoS ONE, 2017-02-09;12(2):e0171748. 2017-02-09 [PMID: 28182766] (IHC, Mouse)	IHC	Mouse
Christopher R. Hansebout, Caixin Su, Kiran Reddy, Donald Zhang, Cai Jiang, Michel P. Rathbone et al. Enteric glia mediate neuronal outgrowth through release of neurotrophic factors Neural Regen Res 10/5/2012 [PMID: 25538736]
Wang T, Bruggeman K, Sheean R, Turner B, Nisbet D, Parish C Characterization of the stability and bio-functionality of tethered proteins on bioengineered scaffolds: implications for stem cell biology and tissue repair. J Biol Chem, 2014-04-03;289(21):15044-51. 2014-04-03 [PMID: 24700461] (ELISA Development (Detection), N/A)	ELISA Development (Detection)	N/A
Sarin S, Boivin F, Li A, Lim J, Svajger B, Rosenblum N, Bridgewater D beta-Catenin overexpression in the metanephric mesenchyme leads to renal dysplasia genesis via cell-autonomous and non-cell-autonomous mechanisms. Am J Pathol, 2014-03-15;184(5):1395-410. 2014-03-15 [PMID: 24637293] (IHC-P, Mouse)	IHC-P	Mouse
Quintino L, Manfre G, Wettergren E, Namislo A, Isaksson C, Lundberg C Functional neuroprotection and efficient regulation of GDNF using destabilizing domains in a rodent model of Parkinson's disease. Mol Ther, 2013-07-24;21(12):2169-80. 2013-07-24 [PMID: 23881415] (IHC-Fr, Western Blot, Human, Rat)	IHC-Fr, Western Blot	Human, Rat
Susanne Bäck, Johan Peränen, Emilia Galli, Päivi Pulkkila, Liina Lonka-Nevalaita, Tuulia Tamminen et al. Gene therapy with AAV2-CDNF provides functional benefits in a rat model of Parkinson's disease Brain and Behavior 3/1/2013 [PMID: 23532969]
Taylor H, Barua N, Bienemann A, Wyatt M, Castrique E, Foster R, Luz M, Fibiger C, Mohr E, Gill S Clearance and toxicity of recombinant methionyl human glial cell line-derived neurotrophic factor (r-metHu GDNF) following acute convection-enhanced delivery into the striatum. PLoS ONE, 2013-03-20;8(3):e56186. 2013-03-20 [PMID: 23526931] (IHC, Rat)	IHC	Rat
Somayeh Ahmadiantehrani, Dorit Ron Dopamine D2 receptor activation leads to an up-regulation of glial cell line-derived neurotrophic factor via G beta gamma -Erk1/2-dependent induction of Zif268 Journal of Neurochemistry 4/1/2013 [PMID: 23373701]
Ohshima-Hosoyama S, Simmons HA, Goecks N A Monoclonal Antibody-GDNF Fusion Protein Is Not Neuroprotective and Is Associated with Proliferative Pancreatic Lesions in Parkinsonian Monkeys. PLoS ONE, 2012-06-20;7(6):e39036. 2012-06-20 [PMID: 22745701] (IHC-Fr, Primate - Macaca mulatta (Rhesus Macaque))	IHC-Fr	Primate - Macaca mulatta (Rhesus Macaque)
Bonanomi D, Chivatakarn O, Bai G, Abdesselem H, Lettieri K, Marquardt T, Pierchala BA, Pfaff SL. Ret Is a Multifunctional Coreceptor that Integrates Diffusible- and Contact-Axon Guidance Signals. Cell;148(3):568-82. 2/3/2012 [PMID: 22304922]
Valentina Di Liberto, Giuseppa Mudò, Natale Belluardo mGluR2/3 agonist LY379268, by enhancing the production of GDNF, induces a time-related phosphorylation of RET receptor and intracellular signaling Erk1/2 in mouse striatum Neuropharmacology 9/1/2011 [PMID: 21619889]
Richardson RM, Kells AP, Rosenbluth KH, Salegio EA, Fiandaca MS, Larson PS, Starr PA, Martin AJ, Lonser RR, Federoff HJ, Forsayeth JR, Bankiewicz KS Interventional MRI-guided putaminal delivery of AAV2-GDNF for a planned clinical trial in Parkinson's disease. Mol. Ther., 2011-02-22;19(6):1048-57. 2011-02-22 [PMID: 21343917] (IHC-Fr, Primate - Macaca mulatta (Rhesus Macaque))	IHC-Fr	Primate - Macaca mulatta (Rhesus Macaque)
Ann D. Cohen, Michael J. Zigmond, Amanda D. Smith Effects of intrastriatal GDNF on the response of dopamine neurons to 6-hydroxydopamine: Time course of protection and neurorestoration Brain Research 1/25/2011 [PMID: 21062624]
Hadaczek P, Johnston L, Forsayeth J, Bankiewicz KS Pharmacokinetics and bioactivity of glial cell line-derived factor (GDNF) and neurturin (NTN) infused into the rat brain. Neuropharmacology, 2010-02-11;58(7):1114-21. 2010-02-11 [PMID: 20153340] (IHC-Fr, Rat)	IHC-Fr	Rat
Hongtao Wang, Inna Hughes, William Planer, Alexander Parsadanian, John R. Grider, Bhupinder P. S. Vohra et al. The Timing and Location of Glial Cell Line-Derived Neurotrophic Factor Expression Determine Enteric Nervous System Structure and Function The Journal of Neuroscience 1/27/2010 [PMID: 20107080]
Emborg ME, Moirano J, Raschke J, Bondarenko V, Zufferey R, Peng S, Ebert AD, Joers V, Roitberg B, Holden JE, Koprich J, Lipton J, Kordower JH, Aebischer P Response of aged parkinsonian monkeys to in vivo gene transfer of GDNF. Neurobiol. Dis., 2009-08-04;36(2):303-11. 2009-08-04 [PMID: 19660547] (IHC-Fr, Primate - Macaca mulatta (Rhesus Macaque))	IHC-Fr	Primate - Macaca mulatta (Rhesus Macaque)
Kells AP, Hadaczek P, Yin D, Bringas J, Varenika V, Forsayeth J, Bankiewicz KS Efficient gene therapy-based method for the delivery of therapeutics to primate cortex. Proc. Natl. Acad. Sci. U.S.A., 2009-02-04;106(7):2407-11. 2009-02-04 [PMID: 19193857] (IHC, Primate - Macaca mulatta (Rhesus Macaque))	IHC	Primate - Macaca mulatta (Rhesus Macaque)
Suzuki M, McHugh J, Tork C, Shelley B, Hayes A, Bellantuono I, Aebischer P, Svendsen CN Direct muscle delivery of GDNF with human mesenchymal stem cells improves motor neuron survival and function in a rat model of familial ALS. Mol. Ther., 2008-09-16;16(12):2002-10. 2008-09-16 [PMID: 18797452] (IHC-Fr, Human)	IHC-Fr	Human
Ai X, Kitazawa T, Do AT, Kusche-Gullberg M, Labosky PA, Emerson CP SULF1 and SULF2 regulate heparan sulfate-mediated GDNF signaling for esophageal innervation. Development, 2007-09-01;134(18):3327-38. 2007-09-01 [PMID: 17720696] (IHC-Fr, Mouse)	IHC-Fr	Mouse
Hamra FK, Chapman KM, Nguyen D, Garbers DL Identification of neuregulin as a factor required for formation of aligned spermatogonia. J. Biol. Chem., 2006-11-10;282(1):721-30. 2006-11-10 [PMID: 17098736] (Neutralization, Rat)	Neutralization	Rat
Xu L, Yan J, Chen D, Welsh AM, Hazel T, Johe K, Hatfield G, Koliatsos VE Human neural stem cell grafts ameliorate motor neuron disease in SOD-1 transgenic rats. Transplantation, 2006-10-15;82(7):865-75. 2006-10-15 [PMID: 17038899] (IHC, Rat)	IHC	Rat
Kobayashi T, Ahlenius H, Thored P, Kobayashi R, Kokaia Z, Lindvall O Intracerebral infusion of glial cell line-derived neurotrophic factor promotes striatal neurogenesis after stroke in adult rats. Stroke, 2006-07-27;37(9):2361-7. 2006-07-27 [PMID: 16873711] (IHC-Fr, Rat)	IHC-Fr	Rat
Fouchecourt S, Godet M, Sabido O, Durand P Glial cell-line-derived neurotropic factor and its receptors are expressed by germinal and somatic cells of the rat testis. J. Endocrinol., 2006-07-01;190(1):59-71. 2006-07-01 [PMID: 16837611] (Flow Cytometry, ICC, Western Blot, Rat)	Flow Cytometry, ICC, Western Blot	Rat
Oo TF, Ries V, Cho J, Kholodilov N, Burke RE Anatomical basis of glial cell line-derived neurotrophic factor expression in the striatum and related basal ganglia during postnatal development of the rat. J. Comp. Neurol., 2005-03-28;484(1):57-67. 2005-03-28 [PMID: 15717300] (ICC, IHC-Fr, Rat)	ICC, IHC-Fr	Rat
Esper RM, Loeb JA Rapid axoglial signaling mediated by neuregulin and neurotrophic factors. J. Neurosci., 2004-07-07;24(27):6218-27. 2004-07-07 [PMID: 15240814] (Neutralization, Chicken)	Neutralization	Chicken
Benner EJ, Mosley RL, Destache CJ, Lewis TB, Jackson-Lewis V, Gorantla S, Nemachek C, Green SR, Przedborski S, Gendelman HE Therapeutic immunization protects dopaminergic neurons in a mouse model of Parkinson's disease. Proc. Natl. Acad. Sci. U.S.A., 2004-06-14;101(25):9435-40. 2004-06-14 [PMID: 15197276] (IHC, Mouse)	IHC	Mouse
Sun W, Oppenheim RW Response of motoneurons to neonatal sciatic nerve axotomy in Bax-knockout mice. Mol. Cell. Neurosci., 2003-12-01;24(4):875-86. 2003-12-01 [PMID: 14697655] (Neutralization, Mouse)	Neutralization	Mouse
Eslamboli A, Cummings RM, Ridley RM, Baker HF, Muzyczka N, Burger C, Mandel RJ, Kirik D, Annett LE Recombinant adeno-associated viral vector (rAAV) delivery of GDNF provides protection against 6-OHDA lesion in the common marmoset monkey (Callithrix jacchus). Exp. Neurol., 2003-11-01;184(1):536-48. 2003-11-01 [PMID: 14637123] (IHC, Primate - Callitrix jacchus (Common Marmoset))	IHC	Primate - Callitrix jacchus (Common Marmoset)
Honma M, Namikawa K, Mansur K, Iwata T, Mori N, Iizuka H, Kiyama H Developmental alteration of nerve injury induced glial cell line-derived neurotrophic factor (GDNF) receptor expression is crucial for the determination of injured motoneuron fate. J. Neurochem., 2002-08-01;82(4):961-75. 2002-08-01 [PMID: 12358802] (IHC, Rat)	IHC	Rat
Pina Serra M, 2019, Quartu M, Ambu R, Follesa P, Del Fiacco M Immunohistochemical localization of GDNF in the human hippocampal formation from prenatal life to adulthood. 6811, 2002-02-22;928(1):138-46. 2002-02-22 [PMID: 11844480] (IHC-Fr, Western Blot, Human, Rat)	IHC-Fr, Western Blot	Human, Rat
Show All 46 Publications. Collapse Publications.

Images	Ratings	Applications	Species	Date	Details
Enlarge	5 reviewed by: Verified Customer	IF	Human	12/18/2014	View Summary Application Immunofluorescence Sample Tested Human GDNF bound to the surface of HEK293 cells transfected with the GDNF receptor GFRa1 Species Human
	4 reviewed by: Verified Customer	IP	Human	12/18/2014	View Summary Application Immunoprecipitation Sample Tested N/A Species Human
	4 reviewed by: Verified Customer	WB	Human	12/18/2014	View Summary Application Western Blot Sample Tested Recombinant GDNF binding to 293 cells expressing GDNF receptors or primary hippocampal neurons or brain homogenates Species Human

Array AF-212-NA

• GDNF Antibodies
• GDNF ELISA Kits
• GDNF Lysates
• GDNF Proteins
• GDNF ELISAs
• GDNF Proteins and Enzymes