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GDNF Antibody [Biotin]

 (9 Publications)
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Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA(Det)
Clonality
Polyclonal
Host
Goat
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Unconjugated
AF-212-NA
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
AF-212-NA
Carrier Free
AF-212-NA
Carrier Free
AF-212-SP
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GDNF Antibody [Biotin] Summary

Immunogen
E. coli-derived and Mouse myeloma cell line NS0-derived recombinant human GDNF
Arg109-Ile211
Accession # P39905
Specificity
Detects human GDNF in ELISAs and Western blots. In sandwich immunoassays, less than 30% cross-reactivity with recombinant rat GDNF is observed and less than 0.1% cross-reativity with recombinant human (rh) BDNF, rhCNTF, rhNeurturin, rh beta -NGF, rhNT-3, and rhNT-4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
GDNF
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
Application Notes
ELISA Capture: Human GDNF Antibody (Catalog # MAB212)
ELISA Detection: Human GDNF Biotinylated Antibody (Catalog # BAF212)
Standard:Recombinant Human GDNF (Catalog # 212-GD)
Readout System
Publications
Read Publications using
BAF212 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for GDNF Antibody [Biotin]

  • Astrocyte-derived trophic factor
  • ATF
  • ATF1
  • ATF2
  • GDNF
  • glial cell derived neurotrophic factor
  • glial cell line derived neurotrophic factor
  • glial cell line-derived neurotrophic factor
  • HFB1-GDNF
  • HGDNF
  • HSCR3

Background

Glial Cell Line-derived Neurotrophic Factor (GDNF) is a neurotrophic factor that has been shown to promote the survival of various neuronal subpopulations in both the central as well as the peripheral nervous systems at different stages of their development. Neuronal subpopulations that have been shown to be affected by GDNF include motoneurons, midbrain dopaminergic neurons, Purkinje cells and sympathetic neurons.

Native GDNF, a disulfide-linked homodimeric glycoprotein, is a novel member of the TGF-beta  superfamily. Human GDNF cDNA encodes a 211 amino acid residue prepropeptide that is processed to yield a dimeric protein. Mature human GDNF was predicted to contain two 134 amino acid residue subunits. Mature rat and human GDNF exhibit approximately 93% amino acid sequence identity and show considerable species cross-reactivity. Cells known to express GDNF include Sertoli cells, type 1 astrocytes, Schwann cells, neurons, pinealocytes and skeletal muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for GDNF Antibody (BAF212)(9)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate - Chlorocebus sabaeus (Green Monkey).

We have publications tested in 4 applications: ICC, IHC, IHC-Fr, IHC-P.


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ICC
(1)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
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Human
(1)
Mouse
(1)
Rat
(1)
Primate - Chlorocebus sabaeus (Green Monkey)
(1)
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Showing Publications 1 - 9 of 9.
Publications using BAF212 Applications Species
Marcelo Duarte Azevedo, Naika Prince, Marie Humbert-Claude, Virginia Mesa-Infante, Cheryl Jeanneret, Valentine Golzne et al. Oxidative stress induced by sustained supraphysiological intrastriatal GDNF delivery is prevented by dose regulation Mol Ther Methods Clin Dev 12/14/2023 [PMID: 37766790]
Laura Stahn, Justyna Rasińska, Tilo Dehne, Stefanie Schreyer, Aileen Hakus, Manfred Gossen et al. Sleeping Beauty transposon system for GDNF overexpression of entrapped stem cells in fibrin hydrogel in a rat model of Parkinson’s disease Drug Delivery and Translational Research 6/1/2023 [PMID: 36853436]
RH Baloh, JP Johnson, P Avalos, P Allred, S Svendsen, G Gowing, K Roxas, A Wu, B Donahue, S Osborne, G Lawless, B Shelley, K Wheeler, C Prina, D Fine, T Kendra-Rom, H Stokes, V Manoukian, A Muthukumar, L Garcia, MG Bañuelos, M Godoy, C Bresee, H Yu, D Drazin, L Ross, R Naruse, H Babu, EA Macklin, A Vo, A Elsayegh, W Tourtellot, M Maya, M Burford, F Diaz, CG Patil, RA Lewis, CN Svendsen Transplantation of human neural progenitor cells secreting GDNF into the spinal cord of patients with ALS: a phase 1/2a trial Nature Medicine, 2022-09-05;28(9):1813-1822. 2022-09-05 [PMID: 36064599] (IHC, Rat) IHC Rat
S Dulz, M Bassal, K Flachsbart, K Riecken, B Fehse, S Schlichtin, S Bartsch, U Bartsch Intravitreal Co-Administration of GDNF and CNTF Confers Synergistic and Long-Lasting Protection against Injury-Induced Cell Death of Retinal Ganglion Cells in Mice Cells, 2020-09-11;9(9):. 2020-09-11 [PMID: 32932933] (ICC, Mouse) ICC Mouse
Matthias Luz, Philip C. Allen, John Bringas, Chris Boiko, Diane E. Stockinger, Kristen J. Nikula et al. Intermittent convection-enhanced delivery of GDNF into rhesus monkey putamen: absence of local or cerebellar toxicity Archives of Toxicology 7/1/2018 [PMID: 29785638]
Abdelwahed Chtarto, Marie Humbert-Claude, Olivier Bockstael, Atze T Das, Sébastien Boutry, Ludivine S Breger et al. A regulatable AAV vector mediating GDNF biological effects at clinically-approved sub-antimicrobial doxycycline doses Molecular Therapy - Methods & Clinical Development 3/30/2016 [PMID: 27069954]
Geneviève Gowing, Brandon Shelley, Kevin Staggenborg, Amanda Hurley, Pablo Avalos, Jesse Victoroff et al. Glial cell line-derived neurotrophic factor-secreting human neural progenitors show long-term survival, maturation into astrocytes, and no tumor formation following transplantation into the spinal cord of immunocompromised rats NeuroReport 4/16/2014 [PMID: 24284956]
Elsworth JD, Redmond DE, Leranth C, Bjugstad KB, Sladek JR, Collier TJ, Foti SB, Samulski RJ, Vives KP, Roth RH AAV2-mediated gene transfer of GDNF to the striatum of MPTP monkeys enhances the survival and outgrowth of co-implanted fetal dopamine neurons. Exp. Neurol., 2008-02-15;211(1):252-8. 2008-02-15 [PMID: 18346734] (IHC-Fr, Primate - Chlorocebus sabaeus (Green Monkey)) IHC-Fr Primate - Chlorocebus sabaeus (Green Monkey)
Sajadi A, Bensadoun JC, Schneider BL, Lo Bianco C, Aebischer P Transient striatal delivery of GDNF via encapsulated cells leads to sustained behavioral improvement in a bilateral model of Parkinson disease. Neurobiol. Dis., 2005-11-21;22(1):119-29. 2005-11-21 [PMID: 16300956] (IHC-P, Human) IHC-P Human

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Check out the latest blog posts on GDNF.
The identification of dopaminergic neurons using Tyrosine Hydroxylase in Parkinson's research and LRRK2
Tyrosine hydroxylase (TH) is a crucial enzyme involved in the biosynthesis of dopamine, norepinephrine and epinephrine in the brain.  Specifically, TH catalyzes the conversion of l-tyrosine to l-dihydroxyphenylalanine (l-dopa).  The importance of t...  Read full blog post.
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Bioinformatics

Gene Symbol GDNF
Uniprot