![Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GBP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Western-Blot-NBP1-47768-img0014.jpg "Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GBP2 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).(1:200)")
| Reactivity | HuSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC |
| Clone | OTI5C8 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Concentration | 1.5 mg/ml |
| Immunogen | Full length human recombinant protein of human GBP2 (NP_004111) produced in HEK293T cell. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | GBP2 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 67 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | PBS (pH 7.3), 1.0% BSA and 50% Glycerol |
| Preservative | 0.02% Sodium Azide |
| Concentration | 1.5 mg/ml |
| Purity | Immunogen affinity purified |
![Intracellular Staining by Flow Cytometry Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin-1_AF1152_Flow_Cytometry_11328.jpg)
![Simple Western Galectin-1 Antibody [Unconjugated]](https://images.novusbio.com/images2/Galectin1_AF1152_Simple_Western_16673.jpg)
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![Western Blot STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/STAT1_AF2894_Western_Blot_5226.jpg)
![Simple Western STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/16351.jpg)
![Intracellular Staining by Flow Cytometry STAT1 [p Tyr701] Antibody [Unconjugated]](https://images.novusbio.com/images2/STAT1_AF2894_Flow_Cytometry_8189.jpg)
Secondary Antibodies |
Isotype Controls |
The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
![Immunocytochemistry/Immunofluorescence: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP2.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunocytochemistry-Immunofluorescence-NBP1-47768-img0013.jpg "Immunocytochemistry/Immunofluorescence: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GBP2.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min)](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0017.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human colon tissue within the normal limits using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min)")
![Flow Cytometry: GBP2 Antibody (OTI5C8) [NBP1-47768] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBP2 antibody, and then analyzed by flow cytometry.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Flow-Cytometry-NBP1-47768-img0001.jpg "Flow Cytometry: GBP2 Antibody (OTI5C8) [NBP1-47768] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GBP2 antibody, and then analyzed by flow cytometry.")
![Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP2.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Western-Blot-NBP1-47768-img0002.jpg "Western Blot: GBP2 Antibody (OTI5C8) [NBP1-47768] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GBP2 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GBP2.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GBP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0003.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human breast tissue using anti-GBP2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GBP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0004.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GBP2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GBP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0005.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-GBP2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GBP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0007.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GBP2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human colon tissue using anti-GBP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0008.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human colon tissue using anti-GBP2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human Kidney tissue using anti-GBP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0009.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human Kidney tissue using anti-GBP2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human Ovary tissue using anti-GBP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0010.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human Ovary tissue using anti-GBP2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human prostate tissue using anti-GBP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0011.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human prostate tissue using anti-GBP2 mouse monoclonal antibody.")
![Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human thyroid tissue using anti-GBP2 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-Paraffin-NBP1-47768-img0012.jpg "Immunohistochemistry-Paraffin: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Human thyroid tissue using anti-GBP2 mouse monoclonal antibody.")
![Immunohistochemistry: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min](http://images.novusbio.com/fullsize/GBP2-Antibody-5C8-Immunohistochemistry-NBP1-47768-img0015.jpg "Immunohistochemistry: GBP2 Antibody (OTI5C8) [NBP1-47768] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GBP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min")
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