gamma Tubulin Antibody [Cy3] Summary
Immunogen |
Synthetic peptide: EEFATEGTDRKDVFFY-K, corresponding to amino acids 38-53 of Human gamma Tubulin. |
Localization |
Centrosomal |
Marker |
Centrosome Marker |
Specificity |
Does not react with alpha, beta, omega and eta Tubulin. The IF testing of this product is performed using chicken fibroblasts. We have not performed IF testing using human cells. This antibody is tested in western blotting using the following cell lysates ; U87, HeLa, Jurkat, C2C12, Rat2, MDCK, and A431. |
Isotype |
IgG |
Clonality |
Polyclonal |
Host |
Rabbit |
Gene |
TUBG1 |
Purity |
Immunogen affinity purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- Immunocytochemistry/ Immunofluorescence 1:10-1:2000
|
Application Notes |
IF: Use at a dilution of 1/200. This concentration was determined by staining of methanol/acetonefixed chicken fibroblast cells.Not tested in other applications.Optimal dilutions/concentrations should be determined by the end user. |
Theoretical MW |
48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
Reactivity Notes
The antigen sequence is identical in mouse and rat Gamma-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast Gamma-tubulin).
Packaging, Storage & Formulations
Storage |
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
Buffer |
10 mM PBS (pH 7.4) and 1.0% BSA |
Preservative |
0.09% Sodium Azide |
Purity |
Immunogen affinity purified |
Alternate Names for gamma Tubulin Antibody [Cy3]
Background
Gamma tubulin, a member of the tubulin superfamily, is a ubiquitous and highly conserved protein within the microtubule organizing centre (MTOC). Gamma tubulin is not a component of microtubules, rather it functions as the microtubule nucleator at the MOTC, is responsible for binding microtubule minus ends and mediating the link between microtubles and the centrosome. By binding to the beta tubulin subunit of the tubulin molecule, it establishes the polarity of a microtubule leaving the alpha tubulin subunit exposed at the positive end. The abundance of Gamma tubulin is less than 1% of the level of either alpha or beta tubulin. It shares approximately 28-32% identity with alpha tubulin from various organisms and 32-36% identity with beta tubulins. The detection, localization and characterization of proteins involved in microtubule function is fundamental to the understanding of mitosis, meiosis and the microtubule cytoskeleton.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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