Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Expression of the mitochondrial biogenesis proteins: SIRT1, PGC1alpha, TFAM and SIRT3 in H1299, H1299r, P31 and P31r cell lysates as determined by immunoblot ...read more
Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Analysis of gamma-Tubulin in porcine brain lysate using NBP1-90616.
Western Blot: gamma Tubulin Antibody (TU-32) [NB110-90616] - Differential reactivity of monoclonal antibodies to gamma-tubulin with human gamma-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y ...read more
Western Blot: gamma Tubulin Antibody (TU-32) - BSA Free [NB110-90616] - Comparative mitochondrial abundance as measured by citrate synthase activity, abundance of VDAC1 & cytochrome oxidase subunit 4(A) Citrate synthase ...read more
human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
Epitope
The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Marker
Centrosome Marker
Specificity
The TU-32 recognizes an epitope (amino acids 434-449 in human) within C-terminus of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBG1
Purity
Protein A purified
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48 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using NB110-90616 in the following applications:
Human reactivity reported in scientific literature (PMID: 29212260).
Packaging, Storage & Formulations
Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
0.9% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein A purified
Alternate Names for gamma Tubulin Antibody (TU-32) - BSA Free
gamma Tubulin
Gamma-1-tubulin
Gamma-tubulin complex component 1
GCP-1
TUBG
TUBG1
TUBGCP1
tubulin gamma-1 chain
tubulin, gamma 1
tubulin, gamma polypeptide
Background
The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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