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Galectin-10 Antibody (561603) [Unconjugated]

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Human peripheral blood lymphocytes were stained with Human Galectin‑10 Monoclonal Antibody (Catalog # MAB5447) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B) and Human IL‑2 R alpha APC-conjugated Monoclonal Antibody (Catalog # FAB1020A). Quadrant markers were set based on control antibody staining (Catalog # IC0041F and IC003A, respectively).
Human peripheral blood lymphocytes were stained with Human Galectin‑10 Monoclonal Antibody (Catalog # MAB5447) followed by Fluorescein-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0103B) and Human IL‑2 R ...read more
Western blot shows lysates of human eosinophils (enriched, approximately 60%). PVDF Membrane was probed with 1 µg/mL of Human Galectin-10 Monoclonal Antibody (Catalog # MAB5447) followed by HRP-conjugated Anti-Mouse ...read more
Galectin-10 was detected in immersion fixed HL-60 human acute promyelocytic leukemia cell line using Mouse Anti-Human Galectin-10 Monoclonal Antibody (Catalog # MAB5447) at 8 µg/mL for 3 hours at room temperature. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
561603
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB5447U-100UG
Alexa Fluor 405
FAB5447V-100UG
Alexa Fluor 488
FAB5447G-100UG
Alexa Fluor 532
FAB5447X
Alexa Fluor 594
FAB5447T-100UG
Alexa Fluor 647
FAB5447R-100UG
Alexa Fluor 700
FAB5447N-100UG
Alexa Fluor 750
FAB5447S-100UG
Allophycocyanin
FAB5447A
Allophycocyanin/Cy7
FAB5447APCCY7
Biotin
FAB5447B
CoraFluor 1
FAB5447CL1
DyLight 350
FAB5447D
DyLight 405
FAB5447E
DyLight 488
FAB5447K
DyLight 550
FAB5447L
DyLight 594
FAB5447M
DyLight 650
FAB5447W
DyLight 680
FAB5447Y
DyLight 755
FAB5447Z
FITC
FAB5447F
HRP
FAB5447H
Janelia Fluor 525
FAB5447JF525
Janelia Fluor 549
FAB5447I
Janelia Fluor 585
FAB5447JF585
Janelia Fluor 635
FAB5447JF635
Janelia Fluor 646
FAB5447J
Janelia Fluor 669
FAB5447JF669
PE
FAB5447P
PE/Atto594
FAB5447PEATT594
PE/Cy5.5
FAB5447PECY55
PE/Cy7
FAB5447PECY7
PerCP
FAB5447C
mFluor Violet 450 SE
FAB5447MFV450
mFluor Violet 500 SE
FAB5447MFV500
mFluor Violet 610 SE
FAB5447MFV610
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB5447-SP
Carrier Free
FAB5447V-100UG
Carrier Free
FAB5447U-100UG
Carrier Free
FAB5447T-100UG
Carrier Free
FAB5447S-100UG
Carrier Free
FAB5447R-100UG
Carrier Free
FAB5447N-100UG
Carrier Free
FAB5447G-100UG
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Galectin-10 Antibody (561603) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human Galectin-10
Met1-Arg142
Accession # Q05315
Specificity
Detects human Galectin-10 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Galectin-1, -2,
-3, -4, -7, -8, or -9/Ecalectin is observed.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CLC
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Galectin-10 Antibody (561603) [Unconjugated]

  • Charcot-Leyden crystal proteinLGALS10A
  • CLC
  • EC 3.1.1.5
  • eosinophil lysophospholipase
  • GAL10
  • Gal-10
  • galectin 10
  • Galectin10
  • Galectin-10
  • LGALS10
  • LPPL_HUMAN
  • Lysolecithin acylhydrolase
  • MGC149659

Background

Galectin-10 (also eosinophil lysophospholipase and Charcot-Leyden Crystal protein) is a 16 kDa member of the lectin family of proteins. It is expressed intracellularly by eosinophils, basophils and CD25+ Treg cells. Although originally believed to possess lysophospholipase activity, this has been shown to be incorrect. It is known to bind lysophospholipase and its inhibitors, and to bind mannose in a very unusual manner. Human Galectin-10 is 142 amino acids (aa) in length. There is one galectin domain (aa 6-138) that contains two dimerization motifs (aa 6-10 and 131-135). Two molecular weight isoforms of 15 and 14 kDa have been described. Human Galectin-10 has no known structural counterpart in rodents.    

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Galectin-10
Antibody (561603) [Un ...
MAB5447
Galectin-10 Antibody (561603) [Unconjugated]
Species: Hu
Applications: WB, Flow, CyTOF-ready, ICC/IF

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Bioinformatics

Gene Symbol CLC
Uniprot