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GADD34 Antibody (OTI3D12) [Janelia Fluor® 549]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Ca, PmSpecies Glossary
Applications WB, Flow, ICC/IF, IHC
Clone
OTI3D12
Clonality
Monoclonal
Host
Mouse
Conjugate
Janelia Fluor 549

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GADD34 Antibody (OTI3D12) [Janelia Fluor® 549] Summary

Immunogen
Full length recombinant protein of human GADD34 (NP_055145) produced in HEK293T cells.
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
PPP1R15A
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for GADD34 Antibody (OTI3D12) [Janelia Fluor® 549]

  • GADD34
  • GADD34Growth arrest and DNA damage-inducible protein GADD34
  • growth arrest and DNA-damage-inducible 34
  • Myeloid differentiation primary response protein MyD116 homolog
  • PPP1R15A
  • protein phosphatase 1 regulatory subunit 15A
  • protein phosphatase 1, regulatory (inhibitor) subunit 15A

Background

GADD34 is a phosphatase whose expression is increased by exposure of cells to DNA damage or stress induced growth arrest. Induction is correlated with apoptosis following exposure to ionizing radiation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for GADD34 Antibody (NBP2-71765JF549) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPP1R15A