![Western Blot: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD34.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Western-Blot-NBP2-71765-img0003.jpg "Western Blot: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GADD34 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GADD34.")
| Reactivity | Hu, Mu, Rt, Ca, PmSpecies Glossary |
| Applications | WB, Flow, ICC/IF, IHC, CyTOF-ready |
| Clone | OTI3D12 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Format | Azide and BSA Free |
| Concentration | LYOPH |
| Immunogen | Full length human recombinant protein of human PPP1R15A(NP_055145) produced in HEK293T cell. |
| Isotype | IgG2b |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | PPP1R15A |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Theoretical MW | 73.3 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
| Storage | Store at -20C. Avoid freeze-thaw cycles. |
| Buffer | Lyophilized from PBS (pH 7.3) with 8% Trehalose |
| Preservative | No Preservative |
| Concentration | LYOPH |
| Purity | Immunogen affinity purified |
| Reconstitution Instructions | we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process. |
Secondary Antibodies |
Isotype Controls |
Research Areas for GADD34 Antibody (NBP2-71765)Find related products by research area.
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The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.
| Gene Symbol | PPP1R15A |
![Immunocytochemistry/Immunofluorescence: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD34.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Immunocytochemistry-Immunofluorescence-NBP2-71765-img0005.jpg "Immunocytochemistry/Immunofluorescence: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY GADD34.")
![Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Human prostate tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71765-img0010.jpg "Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Human prostate tissue using anti-GADD34 mouse monoclonal antibody.")
![Western Blot: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PPP1R15A monoclonal antibody (1:200).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Western-Blot-NBP2-71765-img0001.jpg "Western Blot: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-PPP1R15A monoclonal antibody (1:200).")
![Western Blot: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Western-Blot-NBP2-71765-img0002.jpg "Western Blot: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Analysis of extracts (35ug) from 9 different cell lines by using anti-GADD34 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).")
![Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71765-img0006.jpg "Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GADD34 mouse monoclonal antibody.")
![Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71765-img0007.jpg "Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-GADD34 mouse monoclonal antibody.")
![Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71765-img0008.jpg "Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-GADD34 mouse monoclonal antibody.")
![Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Human pancreas tissue using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71765-img0009.jpg "Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Human pancreas tissue using anti-GADD34 mouse monoclonal antibody.")
![Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Human tonsil using anti-GADD34 mouse monoclonal antibody.](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-Immunohistochemistry-NBP2-71765-img0011.jpg "Immunohistochemistry: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - Staining of paraffin-embedded Human tonsil using anti-GADD34 mouse monoclonal antibody.")
![CyTOF-ready: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue).](http://images.novusbio.com/fullsize/GADD34-Antibody-OTI3D12-Azide-and-BSA-Free-CyTOF-ready-NBP2-71765-img0004.jpg "CyTOF-ready: GADD34 Antibody (OTI3D12) - Azide and BSA Free [NBP2-71765] - HEK293T cells transfected with either RC200581 overexpress plasmid(Red) or empty vector control plasmid(Blue).")
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