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G Protein alpha z Antibody

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Orthogonal Strategies: Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Analysis in human cerebral cortex and liver tissues. Corresponding G Protein alpha z RNA-seq data are presented for ...read more
Western Blot: G Protein alpha z Antibody [NBP1-88111] - Analysis in control (vector only transfected HEK293T lysate) and GNAZ over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian ...read more
Immunocytochemistry/ Immunofluorescence: G Protein alpha z Antibody [NBP1-88111] - Staining of human cell line U-2 OS shows localization to vesicles. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Staining of human pancreas shows strong membranous positivity in islets of langerhans.
Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Staining of human cerebellum shows strong cytoplasmic positivity in molecular layer.
Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Staining of human cerebral cortex shows strong cytoplasmic positivity in neuropil.
Immunohistochemistry-Paraffin: G Protein alpha z Antibody [NBP1-88111] - Staining of human liver shows no positivity in hepatocytes as expected.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

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G Protein alpha z Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: YNAIDSLTRIIRALAALRIDFHNPDRAYDAVQLFALTGPAESKGEITPELLGVMRRLWADPGAQACFSRSSEYHLEDNAAYYLNDLERIAAADYIPTVEDILRSRDMTTGIVENKFTFKELTFKMVDVGGQRSERKKWIHCFEGVTAIIF
Predicted Species
Mouse (98%), Rat (98%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
GNAZ
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
G Protein alpha z Protein (NBP1-88111PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for G Protein alpha z Antibody

  • G(x) alpha chain
  • guanine nucleotide binding protein (G protein), alpha z polypeptide
  • guanine nucleotide-binding protein G(z) subunit alpha
  • gz-alpha
  • transducin alpha

Background

Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site. G protein z alpha, encoded by the GNAZ gene, is enriched in neural tissue and differs in sequence from other G alpha subunits. Alpha Subunits are encoded in 15 genes and several transcripts are alternatively spliced. Receptors may discriminate between splice variants; whether splice variants are functionally different in regulating effectors is not known. All alpha subunits appear to be palmitoylated near the N-terminus.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol GNAZ