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FSHR Antibody (FSHR/1400) - Azide and BSA Free

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Immunohistochemistry-Paraffin: FSH R Antibody (FSHR/1400) [NBP2-54329] - Formalin-fixed, paraffin-embedded human Uterine Carcinoma stained with FSH Receptor Monoclonal Antibody (FSHR/1400).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
FSHR/1400
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
Azide and BSA Free
Concentration
1 mg/ml

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FSHR Antibody (FSHR/1400) - Azide and BSA Free Summary

Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-53199).

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Immunogen
Recombinant human FSHR protein (Uniprot: P23945)
Localization
Cytoplasmic & Cell Surface
Marker
Ovarian Marker
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
FSHR
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot
Application Notes
Immunohistochemistry (Formalin-fixed): 0.1-0.2ug/ml for 30 min at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Use in Western Blot reported in scientific literature (PMID:31832114).
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
10 mM PBS
Preservative
No Preservative
Concentration
1 mg/ml
Purity
Protein A or G purified

Alternate Names for FSHR Antibody (FSHR/1400) - Azide and BSA Free

  • follicle stimulating hormone receptor
  • follicle-stimulating hormone receptor
  • Follitropin receptor
  • FSH R
  • FSH receptor
  • FSHR
  • FSH-R
  • LGR1
  • LGR1FSHRO
  • MGC141667
  • MGC141668
  • ODG1

Background

Follicle-stimulating hormone (FSH), also called follitropin, belongs to the family of glycoprotein hormones that also includes luteininizing hormone and thyroid-stimulating hormone. These hormones are secreted by the pituitary and exist as heterodimers, consisting of a common alpha subunit and a homologous but distinct beta subunit. While the alpha subunit of FSH is involved in the binding of FSH to the receptor, follicle-stimulating hormone receptor (FSHR), the beta subunit stabilizes this interaction. This heterodimer regulates a variety of processes, including secretion, post-translational modification and signal transduction. Both FSH and FSHR are localized to Sertoli cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Video Protocols

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol FSHR