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Frizzled-3 Antibody (169310) [Janelia Fluor® 646]

Name: Frizzled-3 Antibody (169310) [Janelia Fluor&#174; 646]
Brand: Novus Biologicals
SKU: FAB1001J
Price: 559 USD

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Product Details

Summary

Reactivity	Hu, MuSpecies Glossary
Applications	Flow, IHC, CyTOF-ready
Clone	169310
Clonality	Monoclonal
Host	Rat
Conjugate	Janelia Fluor 646

Order Details

View Available Conjugates

Catalog# & Conjugate	Size	Price
Alexa Fluor 532 FAB1001X

Allophycocyanin/Cy7 FAB1001APCCY7

Biotin BAM1001

CoraFluor 1 FAB1001CL1

DyLight 350 FAB1001D

DyLight 405 FAB1001E

DyLight 488 FAB1001K

DyLight 550 FAB1001L

DyLight 594 FAB1001M

DyLight 650 FAB1001W

DyLight 680 FAB1001Y

DyLight 755 FAB1001Z

FITC FAB1001F

HRP FAB1001H

Janelia Fluor 525 FAB1001JF525

Janelia Fluor 549 FAB1001I

Janelia Fluor 669 FAB1001JF669

PE/Atto594 FAB1001PEATT594

PE/Cy5.5 FAB1001PECY55

PE/Cy7 FAB1001PECY7

PerCP FAB1001C

Unconjugated MAB1001-050

mFluor Violet 450 SE FAB1001MFV450

mFluor Violet 500 SE FAB1001MFV500

mFluor Violet 610 SE FAB1001MFV610

View Available Formulations

Catalog# & Formulation	Size	Price
Standard Formulation MAB1001-050

Carrier Free MAB1001-050

Carrier Free MAB1001-100

Carrier Free MAB1001-SP

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Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 532	FAB1001X
Allophycocyanin/Cy7	FAB1001APCCY7
Biotin	BAM1001
CoraFluor 1	FAB1001CL1
DyLight 350	FAB1001D
DyLight 405	FAB1001E
DyLight 488	FAB1001K
DyLight 550	FAB1001L
DyLight 594	FAB1001M
DyLight 650	FAB1001W
DyLight 680	FAB1001Y
DyLight 755	FAB1001Z
FITC	FAB1001F
HRP	FAB1001H
Janelia Fluor 525	FAB1001JF525
Janelia Fluor 549	FAB1001I
Janelia Fluor 669	FAB1001JF669
PE/Atto594	FAB1001PEATT594
PE/Cy5.5	FAB1001PECY55
PE/Cy7	FAB1001PECY7
PerCP	FAB1001C
Unconjugated	MAB1001-050
mFluor Violet 450 SE	FAB1001MFV450
mFluor Violet 500 SE	FAB1001MFV500
mFluor Violet 610 SE	FAB1001MFV610

Frizzled-3 Antibody (169310) [Janelia Fluor® 646] Summary

Immunogen	Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3 Met1-Val157 Accession # Q61086
Specificity	Detects mouse Frizzled-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Frizzled-1, -4, -7, or -8 is observed. By immunohistochemistry, this antibody also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3. This antibody also detects Frizzled-3 on human HEK293 cells by immunocytochemistry.
Isotype	IgG2a
Clonality	Monoclonal
Host	Rat
Gene	FZD3
Purity	Protein A or G purified from hybridoma culture supernatant
Innovator's Reward	Test in a species/application not listed above to receive a full credit towards a future purchase. Learn about the Innovator's Reward

Applications/Dilutions

Dilutions	CyTOF-ready Flow Cytometry Immunohistochemistry
Application Notes	Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

Storage	Store at 4C in the dark.
Buffer	50mM Sodium Borate
Preservative	0.05% Sodium Azide
Purity	Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for Frizzled-3 Antibody (169310) [Janelia Fluor® 646]

frizzled (Drosophila) homolog 3
frizzled homolog 3 (Drosophila)
Frizzled3
Frizzled-3
Fz-3
FZD3
hFz3

Background

Frizzled-3 is a member of the frizzled gene family. Members of this family encode seven-transmembrane domain proteins that are receptors for the Wingless type MMTV integration site family of signaling proteins. Most frizzled receptors are coupled to the beta-catenin canonical signaling pathway. The function of this protein is unknown, although it may play a role in mammalian hair follicle development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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[Unconjugated]

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Species: Hu, Mu, Rt

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Frizzled-3
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FAB1001J

Species: Hu, Mu

Applications: Flow, IHC, CyTOF-ready

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Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, C6 rat glioma cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF membrane was probed with 1 µg/mL Goat Anti-Human/Mouse/Rat beta -Catenin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1329) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). For additional reference, recombinant human beta -catenin (1 ng) was included. A specific band for beta-Catenin was detected at approximately 95 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

beta-Catenin Antibody [Unconjugated]
AF1329

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Bioinformatics

Gene Symbol

FZD3

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Frizzled-3 Antibody (169310) [Janelia Fluor® 646]

Species Glossary

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Product Details

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Frizzled-3 Antibody (169310) [Janelia Fluor® 646] Summary

Applications/Dilutions

Packaging, Storage & Formulations

Notes

Alternate Names for Frizzled-3 Antibody (169310) [Janelia Fluor® 646]

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for Frizzled-3 Antibody (FAB1001J) (0)

Reviews for Frizzled-3 Antibody (FAB1001J) (0)

Product General Protocols

FAQs for Frizzled-3 Antibody (FAB1001J) (0)

Secondary Antibodies

Isotype Controls

Additional Frizzled-3 Products

Research Areas for Frizzled-3 Antibody (FAB1001J)

Blogs on Frizzled-3

Customers Who Bought This Also Bought

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Bioinformatics