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FOXO3 Overexpression Lysate

Name: FOXO3 Overexpression Lysate
Brand: Novus Biologicals
SKU: NBP2-04597
Price: 679 USD

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Western Blot: FOXO3A Overexpression Lysate (Adult Normal) [NBP2-04597] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for FOXO3A.

Product Details

Summary

Reactivity	HuSpecies Glossary
Applications	WB

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FOXO3 Overexpression Lysate Summary

Description

FOXO3 Transient Overexpression Lysate

Expression Host: HEK293T

Plasmid: RC202894

Accession#: NM_201559

Protein Tag: C-MYC/DDK

You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.

Gene

FOXO3

Applications/Dilutions

Dilutions	Western Blot
Application Notes	This product is intended for use as a positive control in Western Blot. Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
Theoretical MW	71.1 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage	Store at -80C. Avoid freeze-thaw cycles.
Buffer	RIPA buffer

Lysate Details for Array

Type

Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for FOXO3 Overexpression Lysate

AF6q21 protein
AF6q21
DKFZp781A0677
FKHRL1
FKHRL1MGC31925
FKHRL1P2
forkhead box O3
forkhead box protein O3
forkhead homolog (rhabdomyosarcoma) like 1
Forkhead in rhabdomyosarcoma-like 1
forkhead, Drosophila, homolog of, in rhabdomyosarcoma-like 1
FOXO2
FoxO3
FOXO3A
FOXO3Aforkhead box O3A
MGC12739

Background

FOX3 probably functions as a trigger for apoptosis through expression of genes necessary for cell death. Translocation of the FOXO3 gene with the MLL gene is associated with secondary acute leukemia.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol

FOXO3

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

FOXO3 Overexpression Lysate

Species Glossary

Images

Product Details

Order Details

FOXO3 Overexpression Lysate Summary

FOXO3 Transient Overexpression Lysate

Applications/Dilutions

Packaging, Storage & Formulations

Lysate Details for Array

Notes

Alternate Names for FOXO3 Overexpression Lysate

Background

Limitations

Customers Who Viewed This Item Also Viewed...

Publications for FOXO3 Lysate (NBP2-04597) (0)

Reviews for FOXO3 Lysate (NBP2-04597) (0)

Product General Protocols

Video Protocols

FAQs for FOXO3 Lysate (NBP2-04597) (0)

Additional FOXO3 Products

Research Areas for FOXO3 Lysate (NBP2-04597)

Blogs on FOXO3

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Review this Product

Bioinformatics