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FE65 Antibody

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Western Blot: FE65 Antibody [NB100-41392] - (0.1ug/ml) staining of NIH/3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: FE65 Antibody [NB100-41392] - (0.1ug/ml) staining of NIH/3T3 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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FE65 Antibody Summary

Immunogen
Peptide with sequence C-GSLKPKRLGAHTP corresponding to C-Terminus according to NP_001155.1, NP_663722.1.
Specificity
This antibody is expected to recognise both reported isoforms (NP_001155.1 and NP_663722.1).
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
APBB1
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:128000
  • Western Blot 0.1 - 0.3 ug/ml
Application Notes
WB: Approx. 100 kDa band observed in lysates of NIH/3T3 and HeLa (PMID: 9685356).
Control
NIH 3T3 Whole Cell Lysate
FE65 Overexpression Lysate
Publications
Read Publication using NB100-41392.

Reactivity Notes

Expected from sequence similarity: Human.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FE65 Antibody

  • adaptor protein FE65a2
  • amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
  • amyloid beta A4 precursor protein-binding, family B, member 1
  • Fe65
  • FE65amyloid beta A4 precursor protein-binding family B member 1
  • MGC:9072
  • Protein Fe65
  • RIR
  • stat-like protein

Background

The protein encoded by this gene is a member of the Fe65 protein family. It is an adaptor protein localized in the nucleus. It interacts with the Alzheimer's disease amyloid precursor protein (APP), transcription factor CP2/LSF/LBP1 and the low-density lipoprotein receptor-related protein. APP functions as a cytosolic anchoring site that can prevent the gene product's nuclear translocation. This encoded protein could play an important role in the pathogenesis of Alzheimer's disease. It is thought to regulate transcription. Also it is observed to block cell cycle progression by downregulating thymidylate synthase expression. Multiple alternatively spliced transcript variants have been described for this gene but some of their full length sequence is not known.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FE65 Antibody (NB100-41392)(1)


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Control Lysate(s)

NIH 3T3 Whole Cell Lysate
FE65 Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol APBB1
Entrez
Uniprot