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Fc gamma RIII (CD16) Antibody (245536) [Allophycocyanin]

 (2 Publications)
 (1 Review)
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Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Fc gamma RIII (CD16) APC-conjugated Monoclonal Antibody (Catalog # FAB2546A) and Mouse Anti-Human CD14 PE-conjugated Monoclonal Antibody (Catalog # FAB3832P). Quadrant markers were set based on control antibody staining (Catalog # IC003A). View our protocol for Staining Membrane-associated Proteins.
Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Fc gamma RIII (CD16) APC-conjugated Monoclonal Antibody (Catalog # FAB2546A) and Mouse Anti-Human CD14 PE-conjugated Monoclonal ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
245536
Clonality
Monoclonal
Host
Mouse
Conjugate
Allophycocyanin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB2546U-100UG
Alexa Fluor 405
FAB2546V-100UG
Alexa Fluor 488
FAB2546G-100UG
Alexa Fluor 532
FAB2546X
Alexa Fluor 594
FAB2546T-100UG
Alexa Fluor 647
FAB2546R-100UG
Alexa Fluor 750
FAB2546S-100UG
Allophycocyanin
FAB2546A-100
Allophycocyanin/Cy7
FAB2546APCCY7
Biotin
FAB2546B-025
CoraFluor 1
FAB2546CL1
DyLight 350
FAB2546D
DyLight 405
FAB2546E
DyLight 488
FAB2546K
DyLight 550
FAB2546L
DyLight 594
FAB2546M
DyLight 650
FAB2546W
DyLight 680
FAB2546Y
DyLight 755
FAB2546Z
Fluorescein
FAB2546F-100
Fluorescein
FAB2546F-025
HRP
FAB2546H
Janelia Fluor 525
FAB2546JF525
Janelia Fluor 549
FAB2546I
Janelia Fluor 585
FAB2546JF585
Janelia Fluor 635
FAB2546JF635
Janelia Fluor 646
FAB2546J
Janelia Fluor 669
FAB2546JF669
PE/Atto594
FAB2546PEATT594
PE/Cy5.5
FAB2546PECY55
PE/Cy7
FAB2546PECY7
PerCP (Peridinin-chlorophyll Protein Complex)
FAB2546C-100
PerCP (Peridinin-chlorophyll Protein Complex)
FAB2546C-025
Phycoerythrin
FAB2546P-100
Phycoerythrin
FAB2546P-025
Unconjugated
MAB2546-100
mFluor Violet 450 SE
FAB2546MFV450
mFluor Violet 500 SE
FAB2546MFV500
mFluor Violet 610 SE
FAB2546MFV610
View Available Formulations
Catalog# & Formulation Size Price
Standard Formulation
MAB2546-100
Carrier Free
MAB2546-100
Carrier Free
FAB2546R-100UG
Carrier Free
FAB2546S-100UG
Carrier Free
FAB2546T-100UG
Carrier Free
FAB2546U-100UG
Carrier Free
FAB2546V-100UG
Carrier Free
FAB2546B-025
Carrier Free
MAB2546-500
Carrier Free
MAB2546-SP
Carrier Free
FAB2546C-025
Carrier Free
FAB2546A-100
Carrier Free
FAB2546P-100
Carrier Free
FAB2546P-025
Carrier Free
FAB2546F-100
Carrier Free
FAB2546F-025
Carrier Free
FAB2546C-100
Carrier Free
FAB2546G-100UG
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Fc gamma RIII (CD16) Antibody (245536) [Allophycocyanin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16)
Thr20-Gln208
Accession # O75015
Specificity
Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Mouse
Gene
FCGR3A
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
Learn about the Innovator's Reward

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
FAB2546A in the following application:
Publications
Read Publications using
FAB2546A in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Fc gamma RIII (CD16) Antibody (245536) [Allophycocyanin]

  • CD16
  • CD16A
  • Fc fragment of IgG receptor IIIa
  • Fc gamma RIII
  • FCG3
  • FCGR3
  • FcgRIII
  • FCR-10
  • FCRIII
  • FCRIIIA
  • IGFR3
  • IMD20

Background

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Fc gamma RIII (CD16) Antibody (FAB2546A)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: Flow Cytometry.


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Flow Cytometry
(1)
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Human
(1)
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Review for Fc gamma RIII (CD16) Antibody (FAB2546A) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using FAB2546A:
Filter by Applications
Flow
(1)
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Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry Fc gamma RIII (CD16) FAB2546A
Enlarge 		4
reviewed by:
Verified Customer
 Flow Human 03/01/2019
View

Summary

ApplicationFlow Cytometry
Sample TestedHuman PBMC
SpeciesHuman
LotAAIW0318101

Comments

CommentsHuman PBMCs were stained with anti-FcgRIII A/B APC.

Product General Protocols

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FAQs for Fc gamma RIII (CD16) Antibody (FAB2546A) (0)

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Secondary Antibodies

 

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Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human Fc gamma RIII (CD16) PE-conjugated Monoclonal Antibody (Catalog # FAB2546P) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (Catalog # FAB3832A). Quadrant markers were set based on control antibody staining (Catalog # IC003P). View our protocol for Staining Membrane-associated Proteins.
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Recent Reviews

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Flow Cytometry Fc gamma RIII (CD16) FAB2546A
Enlarge
 Verified Customer
03/01/2019
Application: Flow
Species: Human
read this review

Bioinformatics

Gene Symbol FCGR3A
Uniprot