![Knockout Validated: FAT4 Antibody [FITC] [NBP1-78381F] - Representative images show IHC staining with anti-FAT4 antibody (NBP1-78381F) of lung sections from BGC-823-FAT4-KO and BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FAT4-Antibody-[FITC]-Knockout-Validated-NBP1-78381F-img0001.jpg "Knockout Validated: FAT4 Antibody [FITC] [NBP1-78381F] - Representative images show IHC staining with anti-FAT4 antibody (NBP1-78381F) of lung sections from BGC-823-FAT4-KO and BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 um. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ICC/IF, IHC, KO |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | FITC |
| Immunogen | A synthetic peptide made to a C-terminal portion of the human FAT4 protein (between residues 4000-4500) [UniProt Q6V0I7] |
| Localization | Membrane; Single-pass type I membrane protein. Note: In the kidney, localizes to primary cilia. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | FAT4 |
| Purity | Immunogen affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
| Dilutions |
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| Application Notes | Optimal dilution of this antibody should be experimentally determined. |
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| Publications |
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| Storage | Store at 4C in the dark. |
| Buffer | PBS |
| Preservative | 0.05% Sodium Azide |
| Purity | Immunogen affinity purified |
![FAT4 Antibody [FITC]](/sites/all/modules/enterprise-tech/et_datasheets/images/novus_guarantee.png "FAT4 Antibody [FITC]")
| Publication using NBP1-78381F | Applications | Species |
|---|---|---|
| Jiang X, Liu Z, Xia Y et al. Low FAT4 expression is associated with a poor prognosis in gastric cancer patients. Oncotarget 2018-01-12 [PMID: 29435168] (Mouse) | Mouse |
Secondary Antibodies |
Isotype Controls |
Research Areas for FAT4 Antibody (NBP1-78381F)Find related products by research area.
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| Gene Symbol | FAT4 |
![Knockout Validated: FAT4 Antibody [FITC] [NBP1-78381F] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model. Representative images show IHC staining of BGC-823-KO-NC and BGC-823-FAT4-KO derived xenograft tumors with antibody against FAT4 (NBP1-78381F). Scale bar, 100 um. Image collected and cropped by Citeab from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/FAT4-Antibody-[FITC]-Knockout-Validated-NBP1-78381F-img0002.jpg "Knockout Validated: FAT4 Antibody [FITC] [NBP1-78381F] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model. Representative images show IHC staining of BGC-823-KO-NC and BGC-823-FAT4-KO derived xenograft tumors with antibody against FAT4 (NBP1-78381F). Scale bar, 100 um. Image collected and cropped by Citeab from the following publication (//pubmed.ncbi.nlm.nih.gov/29435168/) licensed under a CC-BY license.")
![Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] - H&E & IHC staining of metastatic lung nodules in the nude mice model(A) Representative images (40X, 100X & 400X) of H&E stained lung sections from nude mice injected via the tail vein with BGC-823-FAT4-KO & BGC-823-KO-NC cells. (B) Representative images show IHC staining with anti-FAT4 antibody of lung sections from BGC-823-FAT4-KO & BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 μm. (C) Histogram shows Ki-67 expression & index (%) in lungs of the BGC-823-FAT4-KO & BGC-823-KO-NC group mice. As shown, the BGC-823-FAT4-KO group shows higher Ki-67 index (%) than BGC-823-KO-NC group. Data represent mean ± SD from 3 independent experiments. Scale bar, 100 μm. * denotes P < 0.05 compared to control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78381f_rabbit-polyclonal-fat4-antibody-fitc-310202416154611.jpg "Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] - H&E & IHC staining of metastatic lung nodules in the nude mice model(A) Representative images (40X, 100X & 400X) of H&E stained lung sections from nude mice injected via the tail vein with BGC-823-FAT4-KO & BGC-823-KO-NC cells. (B) Representative images show IHC staining with anti-FAT4 antibody of lung sections from BGC-823-FAT4-KO & BGC-823-KO-NC group mice. FAT4 is positive staining in the lung metastatic tumor nodules of BGC-823-KO-NC group, whereas it negative expresses in BGC-823-FAT4-KO group. Scale bar, 100 μm. (C) Histogram shows Ki-67 expression & index (%) in lungs of the BGC-823-FAT4-KO & BGC-823-KO-NC group mice. As shown, the BGC-823-FAT4-KO group shows higher Ki-67 index (%) than BGC-823-KO-NC group. Data represent mean ± SD from 3 independent experiments. Scale bar, 100 μm. * denotes P < 0.05 compared to control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
![Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model(A) Nude mice xenografted (n=5) with BGC-823-FAT4-KO cells (left side) show increased tumor growth (size) than tumors in mice xenografted with control BGC-803-NC cells (right side). (B) BGC-823-FAT4-KO cell derived xenograft tumors show increased tumor volume than control BGC-823-KO-NC cells (*P<0.05). The data points represent mean tumor volume ± SD measured on the indicated days. (C) Representative images show IHC staining of BGC-823-KO-NC & BGC-823-FAT4-KO derived xenograft tumors with antibodies against FAT4 & Ki-67. Scale bar, 100 μm. (D) Histogram plots show percent Ki-67 positive cells in BGC-823-KO-NC & BGC-823-FAT4-KO derived xenograft tumors. As shown, the Ki-67 index is higher in the BGC-823-FAT4-KO derived tumors than BGC-823-KO-NC derived tumors. Data represent mean ± SD from 3 independent experiments. * denotes P < 0.05 compared to control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.](http://images.novusbio.com/fullsize/nbp1-78381f_rabbit-polyclonal-fat4-antibody-fitc-310202416163734.jpg "Immunohistochemistry: FAT4 Antibody [FITC] [NBP1-78381F] - Effect of FAT4 knockout on GC tumorigenesis in the nude mice xenograft model(A) Nude mice xenografted (n=5) with BGC-823-FAT4-KO cells (left side) show increased tumor growth (size) than tumors in mice xenografted with control BGC-803-NC cells (right side). (B) BGC-823-FAT4-KO cell derived xenograft tumors show increased tumor volume than control BGC-823-KO-NC cells (*P<0.05). The data points represent mean tumor volume ± SD measured on the indicated days. (C) Representative images show IHC staining of BGC-823-KO-NC & BGC-823-FAT4-KO derived xenograft tumors with antibodies against FAT4 & Ki-67. Scale bar, 100 μm. (D) Histogram plots show percent Ki-67 positive cells in BGC-823-KO-NC & BGC-823-FAT4-KO derived xenograft tumors. As shown, the Ki-67 index is higher in the BGC-823-FAT4-KO derived tumors than BGC-823-KO-NC derived tumors. Data represent mean ± SD from 3 independent experiments. * denotes P < 0.05 compared to control. Image collected & cropped by CiteAb from the following publication (//www.oncotarget.com/lookup/doi/10.18632/oncotarget.23702), licensed under a CC-BY license. Not internally tested by Novus Biologicals.")
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![Flow Cytometry: Rabbit IgG Isotype Control [FITC] [NBP2-24892] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 antibody.](https://images.novusbio.com/images/Rabbit-IgG-Isotype-Control-[FITC]-Flow-Cytometry-NBP2-24892-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [FITC] [NBP2-24892] - Analysis of TLR7 in Ramos cells using 2 ug of NBP2-24892. Shaded histogram represents FITC-conjugated rabbit IgG isotype control ; red represents anti-TLR7 antibody.")