F4/80 Antibody (BM8)

F4/80 antigen is a cell surface glycoprotein that is the murine homolog of human EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1) (1,2). Specifically, F4/80 (EMR1) is one of five members belonging to the adhesion G-coupled receptor (ADGRE) subfamily, also known as the epidermal growth factor seven transmembrane (EGF-TM7) receptors (1,2). F4/80 is expressed by the myeloid lineage including in eosinophils, monocytes, macrophages, and myeloid dendritic cells (1,2). The molecular structure of F4/80 contains an EGF-like extracellular domain and a seven transmembrane motif, with a theoretical molecular weight of 160 kDa (1).

Depending on factors such as differentiation, activation, and location, the level of F4/80 expression can vary between cell-types (1,2). For example, peritoneal macrophages are subdivided based on morphology and expression with large peritoneal macrophages having high levels of F4/80 and small peritoneal macrophages having low F4/80 expression (1). Furthermore, splenic macrophages are classified based on location (red pulp, marginal zone, and white pulp) and F4/80 expression level varies within these populations as well; the red pulp macrophages have high levels of F4/80 and CD68 by comparison (1). Originally, the F4/80 monoclonal antibody was generated for a macrophage-specific surface antigen, however F4/80 staining is also observed in other hematopoietic cell populations, thus other immune cell markers should be used in conjunction with F4/80 to distinguish between different populations (1). Though the full extent of F4/80 ligands and biological functions remains elusive, studies have suggested that F4/80 is critical for macrophage-natural killer cell interaction and cytokine release (1,2). Additionally, a study of anterior chamber-associated immune deviation (ACAID) indicated a possible role for F4/80 in the generation of regulatory T cells (1,2).

References

1. Dos Anjos Cassado A. (2017). F4/80 as a Major Macrophage Marker: The Case of the Peritoneum and Spleen. Results and Problems in Cell Differentiation. https://doi.org/10.1007/978-3-319-54090-0_7

2. Hamann, J., Hsiao, C. C., Lee, C. S., Ravichandran, K. S., & Lin, H. H. (2016). Adhesion GPCRs as Modulators of Immune Cell Function. Handbook of Experimental Pharmacology. https://doi.org/10.1007/978-3-319-41523-9_15

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 3 applications: ICC/IF, IF/IHC, IHC.

Showing Publications 1 - 10 of 11. Show All 11 Publications. Collapse Publications.

Publications using NBP1-60140	Applications	Species
Regan, DP;Coy, JW;Chahal, KK;Chow, L;Kurihara, JN;Guth, AM;Kufareva, I;Dow, SW; The Angiotensin Receptor Blocker Losartan Suppresses Growth of Pulmonary Metastases via AT1R-Independent Inhibition of CCR2 Signaling and Monocyte Recruitment J. Immunol. 2019-04-10 [PMID: 30971441] (ICC/IF, Mouse)	ICC/IF	Mouse
Liu X, Zhou H, Huang X et al. A Broad Blockade of Signaling from the IL-20 Family of Cytokines Potently Attenuates Collagen-Induced Arthritis J. Immunol. 2016-09-12 [PMID: 27619991] (IF/IHC, Mouse) Details: F4/80 antibody was used as a macrophage marker in tissue sections from CIA model mice in IHC analysis	IF/IHC	Mouse
Poeckel D, Zemski Berry KA, Murphy RC et al. Dual 12/15- and 5-lipoxygenase deficiency in macrophages alters arachidonic acid metabolism and attenuates peritonitis and atherosclerosis in ApoE knock-out mice J Biol Chem [PMID: 19509298] Details: Used the FITC form of this antibody.
Kobayashi M, Yoshida T, Takeuchi D et al. Gr-1(+)CD11b(+) cells as an accelerator of sepsis stemming from Pseudomonas aeruginosa wound infection in thermally injured mice J Leukoc Biol 2008-06-01 [PMID: 18372338] Details: This citation used the FITC version of this antibody.
Schaller E, Macfarlane AJ, Rupec RA et al. Inactivation of the F4/80 glycoprotein in the mouse germ line. Mol Cell Biol. 2002-11-01 [PMID: 12391169]
Stevceva L, Pavli P, HusbAJ et al. The inflammatory infiltrate in the acute stage of the dextran sulphate sodium induced colitis: B cell response differs depending on the percentage of DSS used to induce it BMC Clin Pathol 1905-06-23 [PMID: 11580872] Details: Used the FITC form of this antibody.
Mackler AM, Iezza G, Akin MR et al. Macrophage trafficking in the uterus and cervix precedes parturition in the mouse. Biol Reprod. 1999-10-01 [PMID: 10491619]
Leenen PJ, de Bruijn MF, Voerman JS et al. Markers of mouse macrophage development detected by monoclonal antibodies. J Immunol Methods. 1994-09-01 [PMID: 8083537]
Malorny U, Michels E, Sorg C et al. A monoclonal antibody against an antigen present on mouse macrophages and absent from monocytes. Cell Tissue Res. 1986-01-01 [PMID: 3948241]
Madi A, Sheinin R, Salomon K et al. interFLOW: maximum flow framework for the identification of factors mediating the signaling convergence of multiple receptors Research Square 2023-10-31 (IHC, Mouse)	IHC	Mouse
de Paula Oliveira Santos B, Trentini MM, Machado RB et al. Advax4 delta inulin combination adjuvant together with ECMX, a fusion construct of three protective mTB antigens, induces a potent Th1 immune response and protects mice against Mycobacterium tuberculosis infection Hum Vaccin Immunother 2017-09-22 [PMID: 28937879] (ICC/IF, Mouse)	ICC/IF	Mouse
Show All 11 Publications. Collapse Publications.

Array NBP1-60140

• F4/80 Antibodies