Novus Biologicals products are now on bio-techne.com

Human ERp72 - Ready-To-Use ELISA Kit (Colorimetric)

Images

 
ELISA: Human ERp72 - Ready-To-Use ELISA Kit (Colorimetric) [NBP3-39032] - Standard Curve Reference

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Suitable Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Standard Curve Range
1.56 - 100 ng/mL (example only; lot dependent)
Sensitivity
0.66 ng/mL (example only; lot dependent)

Order Details

Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Human ERp72 - Ready-To-Use ELISA Kit (Colorimetric) Summary

Description
The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
Assay Length: 3 hours
Standard Curve Range
1.56 - 100 ng/mL (example only; lot dependent)
Sensitivity
0.66 ng/mL (example only; lot dependent)
Assay Type
Sandwich ELISA
Inter-Assay
%CV < 12 (example only; lot dependent)
Intra-Assay
%CV < 10 (example only; lot dependent)
Sample Volume
100 uL
Kit Type
ELISA Kit (Colorimetric)
Gene
PDIA4

Applications/Dilutions

Dilutions
  • ELISA

Packaging, Storage & Formulations

Storage
Storage of components varies. See protocol for specific instructions.

Kit Components

Components
  1. Detection Solution A
  2. Detection Solution B
  3. Instruction manual
  4. Plate sealer for 96 wells
  5. Pre-coated 96T strip plate
  6. Standard Diluent
  7. Standard
  8. Stop Solution
  9. TMB Substrate
  10. Wash Buffer (30 x concentrate)

Alternate Names for Human ERp72 - Ready-To-Use ELISA Kit (Colorimetric)

  • Endoplasmic reticulum resident protein 70
  • Endoplasmic reticulum resident protein 72
  • ER protein 70
  • ERp70
  • ERP70ER protein 72
  • ERp72
  • ERp-72
  • ERP72EC 5.3.4.1
  • intestinal-related)
  • protein disulfide isomerase family A, member 4
  • protein disulfide isomerase related protein (calcium-binding protein
  • protein disulfide isomerase-associated 4
  • protein disulfide-isomerase A4

Background

ERp72 is an endoplasmic reticulum luminal protein that is both a stress protein and a member of the protein disulfide isomerase family of proteins. Sequence analysis of ERp72 shows that it shares sequence identity with PDI (protein disulphide isomerase) at three discrete regions, as well as containing the ER retention signal KEEL at its C-terminus. ERp72 is shown to have three copies of sequence believed to be CGHC-containing active sites, which are also found in PDI. These CGHC sites are critical sites that are involved in the protein disulphide isomerization process. These results suggest that ERp72 is a newly described member of the family of CGHC-containing proteins that functions as a molecular chaperone. This antibody was raised by a genetic immunization technique. Genetic immunization can be used to generate antibodies by directly delivering antigen-coding DNA into the animal, rather than injecting a protein or peptide (Tang et al. PubMed: 1545867; Chambers and Johnston PubMed 12910245; Barry and Johnston PubMed: 9234514). The animal's cells produce the protein, which stimulates the animal's immune system to produce antibodies against that particular protein. A vector coding for a partial fusion protein was used for genetic immunisation of a mouse and the resulting serum was tested in Western blot against an E.coli lysate containing that partial fusion protein. Genetic immunization offers enormous advantages over the traditional protein-based immunization method. DNA is faster, cheaper and easier to produce and can be produced by standard techniques readily amenable to automation. Furthermore, the antibodies generated by genetic immunization are usually of superior quality with regard to specificity, affinity and recognizing the native protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-06274
Species: Ce, Ch, Dr, Hu, Mu, Rt, Sh, Ze
Applications: Flow, ICC/IF, IHC-FrFl, IHC, IHC-P, Simple Western, WB
NBP1-84796
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, KD, WB
NBP1-89394
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB300-517
Species: Bv, Ha, Hu, Pm, Mu, Po, Pm, Rb, Rt
Applications: B/N, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IM, IP, KD, WB
NB600-101
Species: Bv, Ha, Hu, Mu, Pm, Rt
Applications: B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB
NBP2-49086
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB300-619
Species: Bv, Ch, Ha, Hu, Mu, Rt
Applications: ICC, ICC/IF, IHC, IHC-P, IP, WB
NBP2-37761
Species: Hu
Applications: ICC/IF, IHC, IHC-P
AF1543
Species: Hu
Applications: IHC, WB
NBP2-34294
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P
NB100-1965
Species: Av, Bv, Ch, Dr, Gp, Hu, Mu, Po, Rb, Rt, Sh, Xp, Ze
Applications: Flow, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP1-84798
Species: Hu, Mu, Rt
Applications: IHC, IHC-P, WB
NBP1-71702
Species: Ch, Hu, Mu
Applications: ChIP, ChIP, ICC/IF, IHC, IHC-P, IP, Simple Western, WB
NBP1-85965
Species: Hu
Applications: IHC, IHC-P
NB600-1335
Species: Hu, Mu, Pm, Rt
Applications: ChIP, ELISA, Flow, GS, ICC/IF, IHC, IHC-P, IP, KD, Simple Western, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP1-32140
Species: Hu, Mu, Pl, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-07040
Species: Hu
Applications: WB
NBP1-33076
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP3-39032
Species: Hu
Applications: ELISA

Publications for ERp72 ELISA Kit (NBP3-39032) (0)

There are no publications for ERp72 ELISA Kit (NBP3-39032).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for ERp72 ELISA Kit (NBP3-39032) (0)

There are no reviews for ERp72 ELISA Kit (NBP3-39032). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ERp72 ELISA Kit (NBP3-39032) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional ERp72 Products

Research Areas for ERp72 ELISA Kit (NBP3-39032)

Find related products by research area.

Blogs on ERp72

There are no specific blogs for ERp72, but you can read our latest blog posts.
Read our latest blog and use the new citation tool on bio-techne.com

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Review this Product

Be the first to review our Human ERp72 - Ready-To-Use ELISA Kit (Colorimetric) and receive a gift card or discount.

Bioinformatics

Gene Symbol PDIA4