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ERK4/MAPK4 Antibody (355810) [Unconjugated]

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Western blot shows lysates of LNCaP human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for ERK4 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.
Western blot shows lysates of LNCaP human prostate cancer cell line, Raji human Burkitt's lymphoma cell line, and TS1 mouse helper T cell line. PVDF membrane was probed with 2 µg/mL of Rat Anti-Human/Mouse/Rat ERK4 ...read more
ERK4 was detected in immersion fixed Raji human Burkitt's lymphoma cell line using Rat Anti-Human/Mouse/Rat ERK4 Monoclonal Antibody (Catalog # MAB3914) at 3 µg/mL for 3 hours at room temperature. Cells were stained ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF
Clone
355810
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB3914-SP
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ERK4/MAPK4 Antibody (355810) [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human ERK4
Met1-Ala126
Accession # P31152
Specificity
Detects human, mouse, and rat ERK4.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
MAPK4
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ERK4/MAPK4 Antibody (355810) [Unconjugated]

  • EC 2.7.11
  • EC 2.7.11.24
  • ERK3
  • Erk3-related
  • ERK4
  • ERK-4
  • Extracellular signal-regulated kinase 4
  • MAP kinase 4
  • MAP kinase isoform p63
  • MAPK 4
  • MAPK4
  • mitogen-activated protein kinase 4
  • p63Mapk
  • PRKM4
  • PRKM4p63-MAPK

Background

Extracellular signal-regulated kinase 4 (ERK4) is a serine/threonine protein kinase also known as mitogen-activated protein kinase 4 (MAPK4), p63MAPK, and ERK3‑related kinase. ERK4 and the structurally similar ERK3 (MAPK6) lack the phosphoacceptor sequence T-X-Y present in other MAPKs. ERK4 complexes with ERK3 to activate MAPK-activated protein kinase 5 (MK5), also known as PRAK.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MAPK4
Uniprot