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ErbB3/Her3 Antibody (66223) [Phycoerythrin]

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MCF-7 human breast cancer cell line was stained with Mouse Anti-Human ErbB3/Her3 PE-conjugated Monoclonal Antibody (Catalog # FAB3481P, filled histogram) or isotype control antibody (Catalog # IC002P, open histogram). ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow
Clone
66223
Clonality
Monoclonal
Host
Mouse
Conjugate
Phycoerythrin

Order Details

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ErbB3/Her3 Antibody (66223) [Phycoerythrin] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human ErbB3/Her3
Ser20-Thr643
Accession # P21860
Specificity
Detects human ErbB3/Her3 in direct ELISAs. Does not cross-react with recombinant human EGF R.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ERBB3
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Flow Cytometry 10 uL/10^6 cells
Publications
Read Publications using
FAB3481P in the following applications:

Packaging, Storage & Formulations

Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
Buffer
Supplied in a saline solution containing BSA and Sodium Azide.
Preservative
Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ErbB3/Her3 Antibody (66223) [Phycoerythrin]

  • c-erbB3
  • EC 2.7.10
  • EC 2.7.10.1
  • ErbB3
  • ErbB-3
  • erbB3-S
  • HER3
  • HER3c-erbB-3
  • LCCS2
  • lethal congenital contracture syndrome 2
  • MDA-BF-1
  • MGC88033
  • p180-ErbB3
  • p45-sErbB3
  • p85-sErbB3
  • Proto-oncogene-like protein c-ErbB-3
  • receptor tyrosine-protein kinase erbB-3
  • Tyrosine kinase-type cell surface receptor HER3
  • v-erb-b2 erythroblastic leukemia viral oncogene homolog 3 (avian)

Background

ErbB3, also known as Her3 (Human Epidermal Growth Factor Receptor 3), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the Epidermal Growth Factor (EGF) family of growth factors. Among ErbB family members, ErbB3 is unique in that it contains a defective kinase domain. ErbB3 is expressed in keratinocytes, melanocytes, skeletal muscle cells, embryonic myoblasts and Schwann cells. Monomeric ErbB3 serves as a low affinity receptor for the Heregulins (HRG). ErbB3 heterodimerizes with ErbB2 to form a high affinity receptor complex. In contrast, ErbB3 homodimerization or heterodimerization with ErbB4 forms a low affinity heregulin-binding complex. Because ErbB3 contains a defective kinase domain, the kinase domain of ErbB2 is responsible for initiating the tyrosine phosphorylation signal through the heterodimeric receptor. It has been found that a discrete three amino acid signal in the ErbB3 cytoplasmic domain is critical for transactivation of ErbB2. The cytoplasmic domain of ErbB3 also contains six consensus binding motifs for the SH2 domain of the regulatory p85 subunit of Phosphoinositide 3-Kinase (PI 3-kinase, PI3K) as well as one proline-rich consensus binding motif for the SH3 domain of p85. Human ErbB3 consists of 1342 amino acids (aa) with a 19 aa signal sequence, a 624 aa extracellular domain, a 21 aa transmembrane region, and a 678 aa cytoplasmic domain. ErbB3 appears to play roles in development, cancer, communication at the neuromuscular junction, and regulation of cell growth and differentiation.

  1. Kraus, M.H. et. al. (1989) Proc. Natl. Acad. Sci. USA 86:9193.
  2. Plowman, G.D. et. al. (1990) Proc. Natl. Acad. Sci. USA 87:4905.
  3. Carraway, K.L. 3rd et. al. (1994) J. Biol. Chem. 269:14303.
  4. Emkey, R. and C.R. Kahn (1997) J. Biol. Chem. 272:31172.
  5. Sundaresan, S. et. al. (1998) Endocrinology 139:4756.
  6. Hellyer, N.J. et. al. (1998) Biochem. J. 333:757.
  7. Schaefer, G. et. al. (1999) J. Biol. Chem. 274:859.
  8. Hellyer, N.J. et. al. (2001) J. Biol. Chem. 276:42153.
  9. Schlessinger, J. (2000) Cell 103:211.
  10. Daly, R.J. (1999) Growth Factors 16:255.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ErbB3/Her3 Antibody (FAB3481P)(10)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: Cell Selection, Flow Cytometry.


Filter By Application
Cell Selection
(1)
Flow Cytometry
(8)
All Applications
Filter By Species
Human
(9)
All Species
Showing Publications 1 - 10 of 10.
Publications using FAB3481P Applications Species
JT de Kay, J Carver, B Shevenell, AM Kosta, S Tsibulniko, E Certo, DB Sawyer, S Ryzhov, MP Robich Decreased expression of ErbB2 on left ventricular epicardial cells in patients with diabetes mellitus Cellular Signalling, 2022-05-21;96(0):110360. 2022-05-21 [PMID: 35609807] (Flow Cytometry, Human) Flow Cytometry Human
I De Pauw, F Lardon, J Van den Bo, H Baysal, E Fransen, V Deschoolme, P Pauwels, M Peeters, JB Vermorken, A Wouters Simultaneous targeting of EGFR, HER2 and HER4 by afatinib overcomes intrinsic and acquired cetuximab resistance in head and neck squamous cell carcinoma cell lines Mol Oncol, 2018-05-01;0(0):. 2018-05-01 [PMID: 29603584] (Flow Cytometry, Human) Flow Cytometry Human
S Kéri, C Szabó, O Kelemen Uniting the neuro developmental and immunological hypotheses: Neuregulin 1 receptor ErbB and Toll-like receptor activation in first-episode schizophrenia Sci Rep, 2017-06-23;7(1):4147. 2017-06-23 [PMID: 28646138] (Flow Cytometry, Human) Flow Cytometry Human
Sebastian K. Wandinger, Idoya Lahortiga, Kris Jacobs, Martin Klammer, Nicole Jordan, Sarah Elschenbroich et al. Quantitative Phosphoproteomics Analysis of ERBB3/ERBB4 Signaling PLOS ONE 2016-01-08 [PMID: 26745281]
Cheng H, Terai M, Kageyama K, Ozaki S, McCue P, Sato T, Aplin A Paracrine Effect of NRG1 and HGF Drives Resistance to MEK Inhibitors in Metastatic Uveal Melanoma. Cancer Res, 2015-05-07;75(13):2737-48. 2015-05-07 [PMID: 25952648] (Flow Cytometry, Human) Flow Cytometry Human
Mirkina I, Hadzijusufovic E, Krepler C, Mikula M, Mechtcheriakova D, Strommer S, Stella A, Jensen-Jarolim E, Holler C, Wacheck V, Pehamberger H, Valent P Phenotyping of human melanoma cells reveals a unique composition of receptor targets and a subpopulation co-expressing ErbB4, EPO-R and NGF-R. PLoS ONE, 2014-01-29;9(1):e84417. 2014-01-29 [PMID: 24489649] (Flow Cytometry, Human) Flow Cytometry Human
Mimura , Kousaku, Shiraishi , Kensuke, Mueller , Anja, Izawa , Shinichi, Kua , Ley-Fang, So , Jimmy, Yong , Wei-Peng, Fujii , Hideki, Seliger , Barbara, Kiessling , Rolf, Kono , Koji The MAPK pathway is a predominant regulator of HLA-A expression in esophageal and gastric cancer. J Immunol, 2013-11-15;191(12):6261-72. 2013-11-15 [PMID: 24244023] (Flow Cytometry, Human) Flow Cytometry Human
MacLeod S, Elgadi M, Bossi G, Sankar U, Pisio A, Agopsowicz K, Sharon D, Graham F, Hitt M HER3 targeting of adenovirus by fiber modification increases infection of breast cancer cells in vitro, but not following intratumoral injection in mice. Cancer Gene Ther, 2012-10-26;19(12):888-98. 2012-10-26 [PMID: 23099884] (Cell Selection, Human) Cell Selection Human
Zhang Y, Gonzalez RM, Zangar RC Protein secretion in human mammary epithelial cells following HER1 receptor activation: influence of HER2 and HER3 expression. BMC Cancer, 2011-02-14;11(0):69. 2011-02-14 [PMID: 21320340] (Flow Cytometry, Human) Flow Cytometry Human
Richards KN, Zweidler-McKay PA, Van Roy N, Speleman F, Trevino J, Zage PE, Hughes DP Signaling of ERBB receptor tyrosine kinases promotes neuroblastoma growth in vitro and in vivo. Cancer, 2010-07-01;116(13):3233-43. 2010-07-01 [PMID: 20564646] (Flow Cytometry, Human) Flow Cytometry Human

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Bioinformatics

Gene Symbol ERBB3
Uniprot