Novus Biologicals products are now on bio-techne.com

Epsilon 1 Tubulin Antibody (5F3B7) - BSA Free

Images

 
Western Blot: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis using TUBE1 mAb against human TUBE1 (AA: 314-472) recombinant protein. (Expected MW is 44.3 kDa)
Immunohistochemistry-Paraffin: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis of paraffin-embedded colon cancer tissues using Epsilon 1 Tubulin mouse mAb with DAB staining.
Flow Cytometry: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis of Hela cells using TUBE1 mouse mAb (green) and negative control (red).
Western Blot: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - 2: Western blot analysis using TUBE1 mAb against HEK293 (1) and TUBE1 (AA: 314-472)-hIgGFc transfected HEK293 (2) cell lysate.
Immunohistochemistry-Paraffin: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Analysis of paraffin-embedded liver cancer tissues using Epsilon 1 Tubulin mouse mAb with DAB staining.
ELISA: Epsilon 1 Tubulin Antibody (5F3B7) [NBP2-52578] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng).

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, IHC, CyTOF-ready
Clone
5F3B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Format
BSA Free
Concentration
1 mg/ml

Order Details

View Available Formulations
Catalog# & Formulation Size Price
Novus Biologicals is part of Bio-Techne

Shop this product on bio-techne.com

Epsilon 1 Tubulin Antibody (5F3B7) - BSA Free Summary

Immunogen
Purified recombinant fragment of human Epsilon 1 Tubulin (AA: 314-472) expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
TUBE1
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • ELISA 1:10000
  • Flow Cytometry 1:200-1:400
  • Immunohistochemistry 1:200-1:1000
  • Immunohistochemistry-Paraffin 1:200-1:1000
  • Western Blot 1:500-1:2000
Application Notes
This antibody is Cytof ready.
Theoretical MW
53 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Protein G purified

Alternate Names for Epsilon 1 Tubulin Antibody (5F3B7) - BSA Free

  • dJ142L7.2
  • Epsilon 1 Tubulin
  • Epsilon-tubulin
  • FLJ22589
  • FLJ44203
  • TUBE
  • TUBE1
  • TUBEepsilon-tubulin
  • tubulin epsilon chain
  • tubulin, epsilon 1

Background

Microtubules constitute one of the major components of eukaryotic cells cytoskeleton and are involved in many essential processes, including cell division, ciliary and flagellar motility and intracellular transport. Microtubules of the eukaryotic cytoskeleton are composed of a heterodimer of a 223 tubulin. In addition to a 223 tubulin, several other tubulins have been identified, bringing the number of distinct tubulin classes to seven. Most of these tubulins have distinct subcellular localization and an emerging, diverse set of functions. Out of the seven different tubulins, four new members of the tubulin family have been identified including epsilon tubulin. Epsilon tubulin was found to be located in the centriolar area, a localization that is dependent on cell cycle modulation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-77068
Species: Hu, Mu, Rt
Applications: ELISA, ICC/IF, IP, WB
NB100-56566
Species: Bv, Hu, Ma, Mu, Po, Rt
Applications: B/N, ChIP, CyTOF-ready, DB, Dual ISH-IHC, ELISA(Cap), ELISA, Flow-CS, Flow-IC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, KD, KO, PAGE, WB
NBP2-29328
Species: Bv, Hu, Mu, Rt, Xp, Ye, Ze
Applications: BindInhib, B/N, ELISA, Flow, Func-Inh, IHC, In vitro, In vivo
AF2699
Species: Hu, Mu
Applications: Simple Western, WB
NBP1-87390
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP2-59690
Species: Am, Av, Fi, Ma, Ze
Applications: ELISA, ICC/IF, IHC, IHC-Fr, IP, WB
NBP1-88502
Species: Hu
Applications: IHC, IHC-P
MAB8930
Species: Hu
Applications: ICC, Simple Western, WB
NBP1-84928
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB600-930
Species: Hu, Rt
Applications: ELISA, WB
NBP2-00764
Species: Hu, Mu, Rt
Applications: Flow, IHC, IHC-P, WB
NBP2-13657
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-88309
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-47659
Species: Hu
Applications: IHC, IHC-P, WB
NBP2-79775
Species: Hu
Applications: IHC, IHC-P, PA, WB
NBP3-17099
Species: Hu
Applications: IHC, IHC-P

Publications for Epsilon 1 Tubulin Antibody (NBP2-52578) (0)

There are no publications for Epsilon 1 Tubulin Antibody (NBP2-52578).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Epsilon 1 Tubulin Antibody (NBP2-52578) (0)

There are no reviews for Epsilon 1 Tubulin Antibody (NBP2-52578). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Epsilon 1 Tubulin Antibody (NBP2-52578) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Epsilon 1 Tubulin Products

Research Areas for Epsilon 1 Tubulin Antibody (NBP2-52578)

Find related products by research area.

Blogs on Epsilon 1 Tubulin

There are no specific blogs for Epsilon 1 Tubulin, but you can read our latest blog posts.
mFluor Violet Conjugated Antibodies

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Epsilon 1 Tubulin Antibody (5F3B7) - BSA Free and receive a gift card or discount.

Bioinformatics

Gene Symbol TUBE1