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MAPRE1 Antibody

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Western Blot: MAPRE1 Antibody [NBP1-84928] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunocytochemistry/ Immunofluorescence: MAPRE1 Antibody [NBP1-84928] - Staining of human cell line U-251 MG shows localization to cytosol and microtubule organizing center. Antibody staining is shown in green.
Orthogonal Strategies: Immunohistochemistry-Paraffin: MAPRE1 Antibody [NBP1-84928] - Staining in human testis and skeletal muscle tissues using anti-MAPRE1 antibody. Corresponding MAPRE1 RNA-seq data are ...read more
Orthogonal Strategies: Western Blot: MAPRE1 Antibody [NBP1-84928] - Analysis in human cell lines Caco-2 and HeLa. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-PPIB.
Immunohistochemistry-Paraffin: MAPRE1 Antibody [NBP1-84928] - Staining of human skeletal muscle shows low expression as expected.
Immunohistochemistry-Paraffin: MAPRE1 Antibody [NBP1-84928] - Staining of human testis shows high expression.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Validated by:
       

Orthogonal Strategies

 

Order Details

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MAPRE1 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: VAPSLVAPALNKPKKPLTSSSAAPQRPISTQRTAAAPKAGPGVVRKNPGVGNGDDEAAELMQQVNVLKLTVEDLEKERDFYFGKLRNIELICQENEGENDPVLQRIVDILYATDEGFVIPDE
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MAPRE1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
MAPRE1 Protein (NBP1-84928PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for MAPRE1 Antibody

  • adenomatous polyposis coli-binding protein EB1
  • APC-binding protein EB1
  • EB1MGC129946
  • End-binding protein 1
  • MGC117374
  • microtubule-associated protein RP/EB family member 1
  • microtubule-associated protein, RP/EB family, member 1

Background

End -binding protein 1 (EB1) is a member of the RP/EB family and was first identified by its binding to the APC protein which is often mutated in familial and sporadic forms of colorectal cancer. This protein localizes to microtubules, especially the growing ends, in interphase cells. During mitosis, the protein is associated with the centrosomes and spindle microtubules. EB1 also associates with components of the dynactin complex and the intermediate chain of cytoplasmic dynein. Because of these associations, it is thought that EB1 is involved in the regulation of microtubule structures and chromosome stability. [rom NCBI GeneID:22919]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MAPRE1