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Endothelin-1 Antibody - Azide and BSA Free

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Knockout Validated: Endothelin-1 Antibody [NBP3-03533] - Analysis of extracts from normal (control) and EDN1 knockout (KO) HeLa cells, using Endothelin-1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Genetic Strategies: Knockout Validated: Endothelin-1 Antibody [NBP3-03533] - Analysis of extracts from normal (control) and EDN1 knockout (KO) HeLa cells, using Endothelin-1 antibody at 1:1000 dilution. Secondary ...read more

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP3-03533
    • Availability
      Product Discontinued

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Endothelin-1 Antibody - Azide and BSA Free Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 18-212 of human Endothelin-1 (NP_001946.3). APETAVLGAELSAVGENGGEKPTPSPPWRLRRSKRCSCSSLMDKECVYFCHLDIIWVNTPEHVVPYGLGSPRSKRALENLLPTKATDRENRCQCASQKDKKCWNFCQAGKELRAEDIMEKDWNNHKKGKDCSKLGKKCIYQQLVRGRKIRRSSEEHLRQTRSETMRNSVKSSFHDPKLKGKPSRERYVTHNRAHW
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
EDN1
Purity
Affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 1:50-1:100
  • Knockout Validated
  • Western Blot 1:500-1:2000
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.01% Thimerosal
Purity
Affinity purified

Alternate Names for Endothelin-1 Antibody - Azide and BSA Free

  • ARCND3
  • EDN1
  • endothelin 1
  • Endothelin-1
  • ET1
  • HDLCQ7
  • PPET1
  • preproendothelin-1
  • QME

Background

Endothelins (ET) show potent constrictor activity in vascular and non-vascular smooth muscle. This family of 21-amino acid peptides exists in at least three isoforms - ET-1, ET-2, and ET-3, and is produced in endothelial and epithelial cells. ET's can mediate biological effects in cells and tissues, and have been shown to bind to an ET receptor in the lung, kidney, heart, and liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Endothelin-1 Antibody - Azide and BSA Free
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Product General Protocols

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Video Protocols

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Bioinformatics

Gene Symbol EDN1