![Western Blot: Endothelin-1 Antibody (3D6) [H00001906-M01] - Analysis of EDN1 expression in transfected 293T cell line by EDN1 monoclonal antibody (M01), clone 3D6.Lane 1: EDN1 transfected lysate(24 KDa).Lane 2: Non-transfected lysate.](http://images.novusbio.com/fullsize/Endothelin-1-Antibody-3D6-Western-Blot-H00001906-M01-img0005.jpg "Western Blot: Endothelin-1 Antibody (3D6) [H00001906-M01] - Analysis of EDN1 expression in transfected 293T cell line by EDN1 monoclonal antibody (M01), clone 3D6.Lane 1: EDN1 transfected lysate(24 KDa).Lane 2: Non-transfected lysate.")
| Reactivity | Hu, MuSpecies Glossary |
| Applications | WB, ELISA, ICC/IF, IP |
| Clone | 3D6 |
| Clonality | Monoclonal |
| Host | Mouse |
| Conjugate | Unconjugated |
| Description | Quality control test: Antibody Reactive Against Recombinant Protein. |
| Immunogen | EDN1 (AAH09720, 113 a.a. ~ 212 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. SQKDKKCWNFCQAGKELRAEDIMEKDWNNHKKGKDCSKLGKKCIYQQLVRGRKIRRSSEEHLRQTRSETMRNSVKSSFHDPKLKGNPSRERYVTHNRAHW |
| Specificity | EDN1 - endothelin 1 |
| Isotype | IgG2a Kappa |
| Clonality | Monoclonal |
| Host | Mouse |
| Gene | EDN1 |
| Purity | IgG purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Application Notes | Antibody reactivity against transfected lysate and recombinant protein for WB. It has been used for ELISA. |
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| Publications |
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| Storage | Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles. |
| Buffer | In 1x PBS, pH 7.4 |
| Preservative | No Preservative |
| Purity | IgG purified |
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Secondary Antibodies |
Isotype Controls |
Research Areas for Endothelin-1 Antibody (H00001906-M01)Find related products by research area.
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![Immunoprecipitation: Endothelin-1 Antibody (3D6) [H00001906-M01] - Analysis of EDN1 transfected lysate using anti-EDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDN1 MaxPab rabbit polyclonal antibody.](http://images.novusbio.com/fullsize/Endothelin-1-Antibody-3D6-Immunoprecipitation-H00001906-M01-img0003.jpg "Immunoprecipitation: Endothelin-1 Antibody (3D6) [H00001906-M01] - Analysis of EDN1 transfected lysate using anti-EDN1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with EDN1 MaxPab rabbit polyclonal antibody.")
![Sandwich ELISA: Endothelin-1 Antibody (3D6) [H00001906-M01] - Detection limit for recombinant GST tagged EDN1 is approximately 0.3ng/ml as a capture antibody.](http://images.novusbio.com/fullsize/Endothelin-1-Antibody-3D6-Sandwich-ELISA-H00001906-M01-img0004.jpg "Sandwich ELISA: Endothelin-1 Antibody (3D6) [H00001906-M01] - Detection limit for recombinant GST tagged EDN1 is approximately 0.3ng/ml as a capture antibody.")
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