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Endothelial Lipase Antibody

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Western Blot: Endothelial Lipase Antibody [NBP1-05031] - Analysis of Endothelial Lipase in Human Liver lysate using NBP1-05031 at 0.3ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Endothelial Lipase Antibody [NBP1-05031] - Analysis of Endothelial Lipase in Human Liver lysate using NBP1-05031 at 0.3ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

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Endothelial Lipase Antibody Summary

Immunogen
Peptide with sequence C-RFNLRTSKDPEHEG corresponding to internal region according to NP_006024.1.
Epitope
internal region
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
LIPG
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
Application Notes
WB: Approx. 70 kDa band observed in human liver lysates is reported in scientific literature (PMID: 17356047). An additional band of unknown identity was also consistently observed at 30 kDa. This band was successfully blocked by incubation with the immunizing peptide.
Control
Endothelial Lipase Overexpression Lysate
Publications
Read Publications using NBP1-05031.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Endothelial Lipase Antibody

  • EC 3.1.1
  • EC 3.1.1.3
  • EDLendothelial lipase
  • ELlipoprotein lipase H
  • Endothelial cell-derived lipase
  • lipase, endothelial
  • PRO719

Background

The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endothelial Lipase Antibody (NBP1-05031)(2)


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Product General Protocols

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Video Protocols

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Control Lysate(s)

Endothelial Lipase Overexpression Lysate

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol LIPG
Entrez
Uniprot