Elastase Antibody (rCELA3B/1811) [CoraFluor™ 1] Summary
Description |
CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays. |
Additional Information |
Recombinant Monoclonal Antibody |
Immunogen |
Recombinant human Elastase protein fragment (around aa 82-238) (exact sequence is proprietary) (Uniprot: P08861) |
Localization |
Cytoplasmic |
Marker |
Pancreatic Function Marker |
Isotype |
IgG1 Kappa |
Clonality |
Monoclonal |
Host |
Mouse |
Gene |
CELA3B |
Purity |
Protein A or G purified |
Innovator's Reward |
Test in a species/application not listed above to receive a full credit towards a future purchase. |
Applications/Dilutions
Dilutions |
- ELISA
- Immunohistochemistry-Paraffin
- Western Blot
|
Application Notes |
Optimal dilution of this antibody should be experimentally determined. |
Packaging, Storage & Formulations
Storage |
Store at 4C in the dark. Do not freeze. |
Buffer |
PBS |
Preservative |
No Preservative |
Purity |
Protein A or G purified |
Notes
CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254
Alternate Names for Elastase Antibody (rCELA3B/1811) [CoraFluor™ 1]
Background
Elastases form a subfamily of serine proteases that hydrolyze many proteins in addition to elastin. Humans have sixelastase genes which encode the structurally similar proteins elastase 1, 2, 2A, 2B, 3A, and 3B. Unlike otherelastases, elastase 3B has little elastolytic activity. Like most of the human elastases, elastase 3B is secreted fromthe pancreas as a zymogen and, like other serine proteases such as trypsin, chymotrypsin and kallikrein, it has adigestive function in the intestine. Elastase 3B preferentially cleaves proteins after alanine residues. Elastase 3Bmay also function in the intestinal transport and metabolism of cholesterol. Both elastase 3A and elastase 3B havebeen referred to as protease E and as elastase 1, and excretion of this protein in fecal material is frequently usedas a measure of pancreatic function in clinical assays. (provided by RefSeq)
Limitations
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are
guaranteed for 1 year from date of receipt.
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